BIOTECHNOLOGY MODEL RECEIVING RABBITS EMBRYOS IN VITRO WITH USING NANOMATERIALS
O. Shcherbak, S. Kovtun, A. Zyuzyun, A. Osypchuk
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Institute of Animal Breeding and Genetics nd. a. M. V. Zubets of NAAS, Pogrebnjaka str., 1, v. Chubinsky, Boryspil District, Kyiv Region, 08321, Ukraine
Developed and applied biotechmodel receiving rabbit’s embryos in vitro with using nanobiomaterials for improvement of reproduction system in farm animals. Showed positive impact using nanomaterial on the basis of ultrafine silica (UFS, aero-sil) on the surface immobilized D- galactosamine on meiotic maturation of oocytes rabbits in vitro. Level of maturation oocytes in vitro under these conditions is 87.1 %. To investigate the usefulness in vitro maturation of oocytes insemination their rabbit epididymal spermatozoa (removed from the tail (epididymis) appendage of the testis). The level fragmentation embryos reached 52.1 %. After 120 hours cultivation to early morula stage evolved 17.0 % embryos. For further evaluation of in vitro biological activity of 0.001 % ing concentration UFS/D-galactosamin we used freshly rabbit epididymal spermatozoa. It is shown that after exposure of spermatozoa in the medium with the addition of 0.001 % concentration UFS/D-galactosamine over 15 minutes decreased activity gametes by 5.0% while preserving that level for three hours. The overall survival time of spermatozoa without adding nanomaterial reached nine hours, and with it — does not exceed eight hours. Addition UFS/D-galactosamine in medium to a concentration of 0.001 % caused a rapid saturation of cell receptors spermatozoa these nanomaterial, which in turn led to a decrease in their activity to 35 %. But this level of activity of sperm preserved longer compared to the control, which is important for improving fertilization in vitro. Established prospects for further biotechnological research with using nanomaterials based UFS and biomolecules in the system preservation and rational use genetic resources farm animals.
Keywords: RABBITS, OOCYTE-CUMULUS COMPLEXES, NANOMATERIALS, ULTRAFINE SILICA, D-GALACTOSAMINE, MORPHOLOGICAL ANALYSIS, CYTOGENETIC ANALYSIS, EPIDIDYMAL SPERMATOZOA, EMBRYOS, IN VITRO FERTILIZATION
