Bìol. Tvarin, 2016, vol. 18, no. 1, pp. 126–130

PROPHYLAXIS OF OXIDATIVE STRESS AND CORRECTIONOF ANTIOXIDANT PROTECTION OF POULTRY POISONED BY NEOVERM

Yu. Svitlychna-Kulak

This email address is being protected from spambots. You need JavaScript enabled to view it.

Kharkiv state zooveterinary academy,
Mala Danylivka, Dergachy district, Kharkiv region, 62341, Ukraine

The data on the effect of a new antihelminthic preparation «Neoverm» (a derivative of avermectins) on hens have been presented in the article. The preparation was administered at a single toxic dose.

It has been found out that as a result of the production of a higher level of POL products in the blood of the fowl there was not enough potential of the fowl’s own resources of AOS to prevent the influence of AMO and the corresponding inclusion of protective antiradical mechanisms but the above process can be corrected with the help of the preparation «E-selen» at 5-fold dose.

The mechanism of proоxidant action of «Neoverm» at a toxic dose (0.4 cm3/kg body weight) was to form the surplus of toxic products of lipid peroxidation — DC and MDA (on average by 2,5 and 2,2 times (P≤0,05), respectively, as compared to the values of the above indices in the control hens) — when the fowl’s own antioxidant resources were lost because of the inhibition of catalase activity and the reduction of the level of structural antioxidants — vitamin E, selen and catalase activity (by 1.9, 0.23 (P≤0,05) and 2.3 times (P≤0,01), respectively) that demonstrates the formation of oxidative stress in the experimental hens.

One can assert the expressed antioxidant effect of the preparation «E-selen» by the results of the restoration of the pool of endogenic antioxidant resources (intensification of catalase activity by 1.9 times (P≤0,01) as compared to the fowl that received only «Neoverm», the normalization of the general AOA to physiological level, increase in the concentration of structural components of non-fermentative part of AOS — vitamin E and selen by 6.1 and 1.3 times (P≤0,001), respectively) in the blood of hens.

Keywords: NEOVERM, HENS, PEROXIDATION OF LIPIDS (POL), DIEN CONJUGATES (DC), MALONE DIALDEHYDE (MDA), ANTIOXIDANT SYSTEM (AOS), ANTIOXIDATION ACTYVITY (AOA), E-SELEN, VITAMIN E, ACTIVE FORMS OF OXYGEN (AFO)

1. Garçon G., Dagher Z., Zerimech F., Ledoux F., Courcot D., Aboukais A., Puskaric E., Shirali P. Dunkerque City air pollution particulate matter — induced cytotoxicity, oxidative stress and inflammation in human epithelial lung cells. Toxicol. in vitro, 2006, 20, no. 4, p. 519–529. https://doi.org/10.1016/j.tiv.2005.09.012
2. Ajayan, P. M., Zhou O. Z. Drug delivery and biomolecular transport. Carbon, 2005, vol. 43, p. 389–415.
3. Dubinina E. E. Role of active forms of oxygen as signal molecules in tissue metabolism at the state of oxidative stress. Problems of med. chemistry, 2001, vol. 47, no. 6, p. 561–581. (in Russian)
4. Zenkov N. A., Lankin V. Z., Menshikova E. B. Oxidative stress: biochemical and pathophysiological aspect. Moscow, Maik, 2001, 343 p. (in Russian)
5. Stegniy B. T., Kovalenko L. V., Romanko M. E. Methods of lipid peroxidation intensity evaluation and its regulation in biological objects: methodical recommendations. Kharkiv, 2007, 59 p. (in Ukrainian)
6. Gavrilova V. B., Mishkorudnaya M. I. Spectrophotometric determination of the content of lipid hydroperoxides in blood plasma. Labor. service, 1985, no. 3, p. 33–35.
7. Korolyuk M. A. Determination of catalase activity. Labor. service, 1988, no. 1, p. 16–18. (in Ukrainian)
8. Klebanov G. I., Babenkova M. V., Teselkin Yu. O. Evaluation of antioxidant activity of blood plasma with the use of bile lipoproreids. Labor. service, 1988, no. 5, p. 59–62. (in Russian)
9. Kutsan O. T., Orobchenko O. L. Control of provision and optimization of vitamin E and selen doses when growing laying hens: methodical recommendations. 2009, 47 p. (in Ukrainian)
10. Malinin O. O., Kutsan O. T., Shevtsova G. M. Determination of inorganic elements in biological substrates by X-ray-flourescent analysis: methodical recommendations. 2009, 40 p. (in Ukrainian)

Download full text in PDF format

Search