Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 66–70
http://dx.doi.org/10.15407/animbiol18.03.066
DEVELOPMENT OF THE IMMUNOSENSOR SYSTEM FOR EXPRESS-DIAGNOSIS OF PSEUDOMONAS AERUGINOSA
O.Yu.Novgorodova, M. F. Starodub
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National University of Life and Environmental Science of Ukraine,
15Heroyiv Oborony Street, Kyiv 03041, Ukraine
The article presents the results of development of the immune biosensor test systems for the express diagnostics of bacteria Pseudomonas aeruginosa, their detection in biological material and in the environment.
Assessment of P. aeruginosa was carried out using an analytical device — immunosensor with immobilized specific antibodies on the transducer surface. The antibodies interact with cell antigens, and the resulting shift value resonance angle is recorded. Changing the angle depends on the amount of the immune complexes formed on the transducer surface. The antibody solution was applied on the prepared transducer surface, and after flushing saline the suspension cells with the appropriate concentration (10 cells in 1 ml and more orders) was applied. The interaction on the surface of immune complexes has been observed. This method can detect at least 10 cells in 1 ml.
In our research we used surface plasmon resonance method. As a transducer a thin film of gold (20 nm) was used, which was applied upon a glass plate by evaporation in vacuum. This surface allows detecting substances in the registration immune interactions with great sensitivity. Statistical significance of the analysis grows sharply with the increasing of concentrations. Linearity of the immunosensor is between 101–106 cells in 1 ml. Sensitivity analysis of the immune analyze can be significantly increased using highly specific affinity monoclonal antibodies.
Keywords: PSEUDOMONAS AERUGINOSA, BACTERIA, SURFACE PLASMON RESONANCE, IMMUNOSENSOR, TRANSDUCER, EXPRESS-DIAGNOSTICS, ANTIGENS, ANTIBODIES
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