Bìol. Tvarin, 2016, volume 18, issue 4, pp. 14–21 http://dx.doi.org/10.15407/animbiol18.04.014
CHARACTERISTICS OF COAGULATION PROCESS IN COWS DURING THE PREGNANCY AND POSTPARTUM PERIODS AND IN COWS WITH POSTNATAL AND OBSTETRICAL PATHOLOGY
S. Vlasenko1, M. Rublenko1, T. Chernyshenko2, O. Gornitska2, T. Platonova2
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1Bila Tserkva National Agrarian University, 8/1 Soborna sq., Bila Tserkva 09111, Ukraine
2 O. V. Palladin Institute of biochemistry NAS of Ukraine, 9 Leontovich str., Kyiv 01011, Ukraine
The proper functioning and adaptation of the hemostatic system is important for the physiological course of pregnancy. Monitoring system for the assessment of blood clotting is not yet fully developed in veterinary medicine. That is why the main goal of the research was to adapt the coagulation methods of laboratory diagnostics and determine their information usefulness while assessing the state of the hemostasis system in pregnant cows at the 35th day, the 3, 5, 7, 8th month of pregnancy and 2–3 days before birth, after birth and during the course of сhronic postpartum metritis and hypotrophy of the ovaries. The screening tests — prothrombin time and activated partial thromboplastin time (APTT) which are designed to characterize the external and internal pathways of blood coagulation, as well as methods for the determination of fibrinogen, soluble fibrin monomer complex (SFMC), products of fibrinogen degradation (FDP), degradation products of fibrinogen (PRF), the activity of protein C have been validated. The use of these techniques has allowed determining the imbalance between pro- and anticoagulant functioning of blood clotting system in cows.
The analysis of the state of the blood clotting system of pregnant cows showed that prothrombin index, fibrinogen level, PRF and protein C activity at all stages of pregnancy and calving period was within the normal limits. The detected reduced clotting time of plasma in the APTT test at the third month of pregnancy and during the postpartum period were accompanied by the appearance in plasma small amount of SFMC (0.001 to 0.01 mkg/ml) which are a specific markers for the activation of blood coagulation system.
Coagulation processes in cows with сhronic postpartum metritis and hypotrophy of the ovaries were characterized by an increased number of markers of thrombophilia — SFMC (from 0.005 to 0.12 mkg/ml) and PRF, a significant reduction in activity of the protein C in 70 % of animals, and a tendency to a decreased fibrinolytic potential (up to 0.41 u/ml at a rate of 1–1.3 ml u/ml). It was found that the informative tests for detecting the activation of the blood clotting system in cows are the determination of the content of soluble fibrin monomer complexes, functionally inactive form of prothrombin and protein C activity. The algorithm for diagnosis of thrombophilia in cows may also include the tests for determining the concentration of fibrinogen, PRF and fibrinolytic potential.
Keywords: COWS PREGNANCY, AFTER PARTURITION, CHRONIC METRITIS, HYPOTROPHY OF THE OVARIES, OXIDE OF NITROGEN, ACUTE PHASE PROTEINS, FIBRINOLYSIS
1. Bondar T. P., Muratova A. Yu., Caturjan E. O. Dynamics of plasma hemostasis in women with thrombogenic pregnancy and delivery complications. Saratov Journal of Medical Scientific Research, 2012, vol. 8, no. 3, pp. 720–723. (in Russian)
2. Chistyakova G. N., Gazieva I. A., Remizova I. I. The content of acute phase proteins in the natural development of physiological pregnancy. Clinical Laboratory Diagnostics, 2006, vol. 4, pp. 20–22. (in Russian)
3. Conway E. M. Thrombomodulin and its role in inflammation. Proc. Semin. in Immunopathology. Vanccuver, 2011, pp. 107–125.
4. Dranik G. N., Ena Ya. M. Vareckaya T. V. Cleavage products of fibrin/fibrinogen in pathological processes. Kyiv, Zdorovya, 1987, 181 p. (in Ukrainian)
5. Fomina L. L. Effect of sex hormones on the functioning of the hemostatic system in cows. Cand. biological sci. diss. Yaroslavl, 2009. 140 p. (in Russian)
6. Franchini M. Haemostasis and pregnancy. J. of Thromb. and Haemost., 2006, 95 (2), pp. 401–413. https://doi.org/10.1160/TH05-11-0753
7. Hansen A. T., Schmidt M., Horvath-Puho E., Pedersen L., Rothman K. Y., Hvas A. M., Sorensen H. T. Preconception venous thromboembolism and placenta-mediated pregnancy complications. J. of Thromb. and Haemost., 2015, 13 (9), pp. 1635–1641. https://doi.org/10.1111/jth.13046
8. Kinzhalova S. V., Pestryaeva L. A., Makarov R. A., Borisova S. V. Hemostasis in pregnant with hypertensive disorders. Tromboz, Gemostaz and Reologia, 2014, vol. 1, no. 57, pp. 70–74. (in Russian)
9. Klycheva M. M., Nazarov S. B., Kuzmenko G. N., Sitnikova O. G., Popova I. G., Malyshkina A. I. Effect of nitric oxide donator and adrenaline on hemocoagulation and its peculiarities at women in late pregnancy. Tromboz, Gemostaz and Reologia, 2015, vol. 4, no. 64, pp. 59–62. (in Russian)
10. Kondashevskaya M. V. Modern views of the role of heparin in hemostasis and regulation of enzymatic and hormonal activities. Annals RAMS, 2010, vol. 7, pp. 35–43. (in Russian)
11. Koroleva D. S., Vinogradova R. P., Chernyshenko T. M., Platonova T. N., Volkov G. L. Using ecamulin-prothrombin activator from the venom of Echis multisquamatis in clinical laboratory diagnostics. Laboratory Diagnostics, 2006, vol. 37, no. 3, pp. 18–22. (in Ukrainian)
12. Krayevskyy A. J. Proteolysis, endotoxicosis and fibrinogen metabolism in the pathogenesis of the obstetrical diseases in cows. Dr. veterinary sci. diss. Kyiv, 2005, 289 p. (in Ukrainian)
13. Kuznik B. I. Cellular and molecular mechanisms of regulation of the hemostatic system in health and disease. Chita, Express-publishing, 2010, 832 p. (in Russian)
14. Lisovenko V. M. Platelet lanka homeostasis in calf cows. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky, 2014, vol. 16, no. 2 (59), 2, pp. 187–193. (in Ukrainian)
15. Momot A. P., Kudinova I. Yu. Elykomov V. A., Semenova N. A., Molchanova I. V., Fadeeva N. I., Medvedeva M. V. Peculiarities of fibrinolytic blood at different terms of physiological pregnancy. Tromboz, Gemostaz and Reologia, 2015, vol. 3, no. 63, pp. 35–40. (in Russian)
16. Momot A. P., Serdiuk G. V., Grigoreva E. E., Nikolaeva M. G. Genetically determined thrombophilias and noncarrying of pregnancy. Tromboz, Gemostaz and Reologia, 2012, vol. 1, no. 49, pp. 60. (in Russian)
17. Moratis S., Taitzoglou I. A., Tsantarliotou M. P., Boscos C. M., Kaldrimidou E., Saratsis Ph. Involvement of the plaminogen activation system in cow endometritis. Theriogenology, 2004, 61, pp. 337–349. https://doi.org/10.1016/S0093-691X(03)00217-6
18. Platonova T. N., Chernyshenko T. M., Savchuk A. N. Determination of the activity of tissue plasminogen activator and the content of soluble fibrin in plasma of the diseased with various pathologies. Laboratory Diagnostics, 2000, vol. 2, pp. 15–18. (in Ukrainian)
19. Platonova T. N., Gornickaya O. V., Chernyshenko T. M., Korolova D. S., Grishhuk V. I., Chernyshenko V. O., Lugovskoy Ye. V. Determination of protein C activity and its role in the clinical laboratory diagnosis. Laboratory Diagnostics, 2013, vol. 3, no. 65, pp. 3–7. (in Ukrainian)
20. Salech O. Optimizing treatment of thrombotic complications in pregnant women and mothers with severe preeclampsia. Cand. medical sci. diss. Donetsk, 2014, 138 p. (in Ukrainian)
21. Sidelnikova V. M., Shmakov R. G. Adaptation and disadaptation mechanisms of hemostasis in pregnancy. Moscow, Triada X, 2004, 192 p. (in Russian)
22. Sokolovska A. S., Platonova T. M., Grynenko T. V., Chernyshenko T. M., Ivanenko T. I. Comparative characteristics of methods for determination of fibrinogen in the blood plasma. Observed. and Clinic. physiology and biochemistry, 2002, vol. 3, pp. 82–86. (in Ukrainian)
23. Stankovic S., Majkic-Singh N. Genetic aspects ofischemic stroke: coagulation, homocysteine, and lipoprotein metabolism aspotential risk factors. Crit. Rev. Clin. Lab. Sci., 2010, 47 (2), pp. 72–123. https://doi.org/10.3109/10408361003791520
24. Tumanyan S. V., Serikov M. E., Tyurmorezov M. Yu., Manych D. Yu. The significance of anesthetic management within the prevention of clinical manifestestations of the systematic response syndrome in patients with severe gestosis. Anesthesiology and reanimotologija, 2004, vol. 1, pp. 32–36. (in Russian)
25. Vareckaya T. V., Mihalovskaya L. I., Svitalskaya L. A., Ena Ya. M. Determination of soluble fibrin in blood plasma. Wedge. of Lab. diagn. Clin. Labor. Diagn., 1992, vol. 7, no. 8, pp. 10–14. (in Ukrainian)
26. Vasilev S. A., Vinogradov V. L., Shutov S. A., Markova M. L. TAFI — antifibrinolytic and anti-inflammatory factor. Tromboz, Gemostaz and Reologia, 2014, vol. 4, no. 60, pp. 12–17. (in Russian)
27. Volkov G. L., Platonova T. N., Savchuk A. N., Gornickaya O. V., Chernyshenko T. M., Krasnobrizhaya E. N. Modern conceptions of hemostasis system. Kyiv, Naukova dumka, 2005, 296 p. (in Ukrainian)
28. Zavalishina S. Yu. Vascular platent interaction in pregnant cows. Fundamental-research, 2015 vol. 2, pp. 267–270. (in Russian)
