Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 79–86
INDICATORS OF HUMORAL IMMUNITY AT HORSES WITH DIFFERENT COURSES OF UVEITIS
A. Mezhenskyi
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State Scientific and Research Institute of Laboratory Diagnostics
and Veterinary and Sanitary Expertise,
30 Donetska str., Kyiv 03151, Ukraine
The article presents results of the research of content of IgA, IgM and IgG, and circulating immune complexes (CIC) in clinically healthy horses (n=10) and horses with acute, subacute and chronic uveitis (n=10). During the research the conditions of feeding, maintenance and operation of horses all groups were similar. The concentration of immunoglobulins was measured by radial immunodiffusion, and the content of fine (<11S), medium (11S–19S) and large (>19S) molecular fractions of CIC — by differential precipitation in 6 %, 3.5 % and 2 % polyethylene glycol solutions.
It is shown that in clinically healthy horses the indicators of humoral immunity were within the reference values. The development of acute uveitis in horses is associated with a significant decrease in the blood serum the content of IgA and IgG in the background increase the content of IgM (disimmunoglobulinemia). Immunoglobulin content at subacute uveitis was not significantly different from that of clinically healthy horses, with the exception of a slight decrease in the number of IgG. The chronic relapsing course of uveitis associated with a significant decrease in the authentic content of IgA and IgM at the same time reducing the apocryphal content of IgG, that is, the development of disimmunoglobulinemia.
Increasing of the level of the CIC (immuno toxicosis syndrome) in horses of all experimental groups at the expense of the most toxigenic medium- and small-molecule fractions wasestablished, whereas the content of large-molecule CIC was not significantly changed in all horses with uveitis. Installed CIC dynamic may be associated with the presence of concomitant diseases (infectious, inflammatory), in which the body activates immune defense mechanisms.
The extent of irregularities of the humoral immune response depends on the course of uveitis, as well as the severity of the clinical manifestations and is the highest in acute and chronic inflammation, while in subacute uveitis, these changes are less pronounced.
Keywords: HORSE, EYE DISEASE, UVEITIS, HUMORAL IMMUNITY, IMMUNOGLOBULINS, CIRCULATING IMMUNE COMPLEXES
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