The Animal Biology

HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF THE F₂ RATS’ ORGANISM IN A PERIOD OF PROLONGED WATERING OF NANO-Ge CITRATE

R.S. Fedoruk¹, U. I. Tesarivska², M. I. Khrabko¹, M. M. Tsap¹, O. P. Dolaychuk¹, S. I. Kropyvka³

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¹Istitute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

²State Scientific-Research Control Institute of Veterinary Medical Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

³Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

In the article the results of experimental studies of the impact of different tenfold (20 and 200 μg Ge) doses of Ge citrate, obtained by nanotechnology method, on hematological and biochemical profile of the organism of the F₂ male rats of second offspring are shown.

It was established that the use of nano-Ge citrate at doses 20 and 200 μg Ge/kg of body weight with drinking water during four months leads to decrease of red blood cell number in both groups of males and to decrease platelets only under action of 20 μg of Ge, with increase of monocytes in animals of this group compared to the control. The possibility of strengthening the deponation functions of the rat organism for erythrocytes and leukocytes under the actions of the Ge citrate were discussed. In the blood of males in the research groups an increase of the albumin, Ca, P, TAG were noted that indicates a stimulatory effect of Ge citrate on its transport function and supplying structural and energy elements for organs and systems. Under the action of nano-Ge citrate in the blood of males in research groups an influence of enzymatic link of the antioxidant support network grows with the increase of SOD and HP-activity and decrease of lipid peroxidation intensity. The applied doses of nano-Ge citrate promote an increase of immunophysiological reactivity of the males’ organisms with the increase of content of middle mass molecules, circulating immune complexes, sialic acid and ceruloplasmin in their blood compared to the concentration of these components in the blood of animals in the control group.

Keywords: HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS, GLYCOPROTEINS, RATS, DOSES OF NANO-Ge CITRATE, BLOOD, WATERING

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INFLUENCE OF PREPARATIONS OF “ACTIVO” AND “PROPOUL” ON COMPOSITION OF CAECUMS MICROFLORA IN PHARAON BREED QUAILS AND THEIR PRODUCTIVITY

О. М. Stefanyshyn, А. V. Hunchak, O. I. Lukovska, Ya. M. Sirko, V. О. Kystsiv, B. B. Lisna, S. I Koretchuk

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The paper presents the data about the caecums microflora composition in quails of Pharaon breed and their productivity under the additional administration of fitobiotic “Activo” and synbiotic “Propoul” to the ration in critical periods of growth (28^th, 41^st, 71^st day after birth). The “Activo” preparation positively affected on the caecums microflora by increasing the number of strains with normal enzymatic activity and growing of cell E. coli. The use of “Propoul” preparation to the quail’s diet caused the changes of caecums microflora composition such as an increase of lactobacilli amount on the 41^st day from the birth. An increase of bifidobacteria and E. coli strains with high enzymatic activity in the quail’s intestinal microflora in this period also had positive influence on the intestinal microbocenosis in poultry. Feeding up the “Activo” and “Propoul” preparations to Pharaon breed quails promoted the productivity of birds of 1^st and 2^nd experimental groups on 8 and 13 % respectively compared with control group during age period.

Pharaon quails belong to meat breeds, but their eggs laying capacity was at the level of eggs breed. High performance of incubation depends on the quality of eggs. The high quality of eggs provides hatchability of quails, their viability and productivity. Administration the “Activo” and “Propoul” preparations to the diet of quail’s in 1^st and 2^nd experimental groups respectively was accompanied by high hatchability on 13 % and 5 % compared with control groups of poultry.

Keywords: QUAILS, INTESTINAL MICROFLORA, PRODUCTIVITY

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DYNAMICS OF BIOCHEMICAL INDICES IN BLOOD DURING EARLY PREGNANCY IN RABBITS UNDER THE INFLUENCE OF DIFFERENT FORMS OF STABILIZED GONADOTROPIC PREPARATIONS

Y. I. Slyvchuk¹, V. Y. Syrvatka¹, I. I. Hevkan¹, O. V. Shtapenko¹, I. O. Matiukha¹, G. O. Milovanova², O. Y. Slyvchuk¹

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¹Institute of Animal Biology, NAAS,
38 V. Stusa str., Lviv 79034, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

²Kyiv National University named after T. Shevchenko,
60 Volodymyrska str., Kyiv 01033, Ukraine

Methodical approaches to creation of new forms of drugs based on stabilized gonadotropins and its activity during prolonged storage were been studied. The influence of injections of stabilized forms of gonadotropin preparations in liposomal forms on the reproductive function of female rabbits during the early stage of pregnancy was determined. The object of the study was human chorionic hormone (hCG) obtained from the urine of pregnant women. The activity of gonadotropin in new forms of drugs stabilized by lysine and sucrose was determined by immuno-chemiluminescent method based on difference between total and free hCG. The effect of stabilizing gonadotropin on reproductive function of female rabbits was determined by the level of estradiol, progesterone and some hematological and biochemical blood parameters in dynamics within two weeks of pregnancy.

It was established that the dispersion leads to loss of 10–12 % of the gonadotropin’s activity however, the duration of dispersion did not influence in its activity. Our results showed that with addition of stabilizers and liposomal form of drug has provided the preservation activity of gonadotropin to 90 % for long-term storage at the temperature of 2–4 °С and at 18–20 °C if taken into account the loss of about 10–12 % after dispersion. The activity of lactate dehydrogenase and concentration of progesterone and estradiol in serum were increased after injections of stabilized preparation during the artificial insemination that indicates its effective action.

Keywords: CHORIONIC GONADOTROPIN, STABILIZATION, RABBIT, PREGNANCY, HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

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THE CONTENT OF FATTY ACIDS IN THE BODIES OF CYPRINUS CARPIO L. IN DIFFERENT CONDITIONS OF LIFE

S. V. Sysolyatin

sergiy_sv@ukr.net

National university of life and environmental sciences of Ukraine,
15 Heroyiv Oborony str., Kyiv 03041, Ukraine

An important role in the processes of adaptation of living organisms to extreme conditions of the environment belongs to fatty acids (FA). By the method of gas chromatography with a flame-ionization detector we have examined the fatty acid composition of liver lipids, gills and brain of Cyprinus carpio L. for normobiosis and artificial hibernation (at 6^th and 24^th hour of exposure). The organ specificity is indicated by the quantitative content of fatty acids of common lipids in the carp organism. For normobiosis, the lipids of the carp brain, unlike other organs, are characterized by higher levels of saturated FA (42.4 %) and by lower level of unsaturated FA (57.6 %) due to the high level of monoenic unsaturated FA (39.3 %) and low polyenic unsaturated FA (18.3 %).

It is established that the influence of the hypoxia-hypercapnic environment on hypothermia (artificial hibernation) causes changes in the amount of fatty acids of the total lipids of carp organs. In particular, there is a decrease in the content of saturated fatty acids, mainly palmitic and stearic, which is probably associated with energy and adaptation processes. The content of unsaturated fatty acids of lipids in the investigated organs increases mainly by polyene, in particular, linoleic, linolenic, arachidonic, eicosapentaenoic and docosahexaenoic. At the same time the level of saturation of fatty acids decreases. The growth of the content of mono unsaturated fatty acids is significant only in the carp liver.

It was found that the growth of the fatty acids content of the ω-3, ω-9 and, especially, ω-6 families, which leads to changes in the ratios of these acids, is modified in the carp organs under the artificial hibernation. The index of lipid metabolism intensity (the ratio of palmitic acid to oleic content (C_16:0/C_18:1ω9)) the most commonly is reduced in liver and gills — in average 54 % for 24-hour hibernation exposure.

A specific reconstruction of the quantitative composition of fatty acids in the lipids of the liver, the gills and the brain of the carp detected under the artificial hibernation may have a compensatory character, which is aimed at supporting their functional activity under other conditions of life.

Keywords: LIVER, GILLS, BRAIN, FATTY ACIDS, CARPS, NORMOBIOSIS, ARTIFICIAL HIBERNATION

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