The Animal Biology

BLOOD INDICES IN COWS WITH DIFFERENT PHYSIOLOGICAL AND PATHOLOGICAL STATES OF THE OVARIES

S. Kornyat, M. Sharan, D. Ostapiv, A. Korbetskyy, I. Jaremchuk, O. Andrushko

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute of Animal Biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

The purpose of this work was to study the functional morphology of cow reproduction system at such ovary’s pathologies as follicular cysts, luteal cysts, persistent corpus luteum, hypofunction of ovaries and during normal cycle (follicular or luteal phases). Blood samples were taken for biochemical studies during the diagnosis making process. In blood samples the activity of alanine and aspartate aminotransferase (ALT and AST); state of the antioxidant system: diene conjugates, the activity of superoxide dismutase (SOD), glutathione peroxidase (GP), hemoglobin content; progesterone and estradiol concentration were determined. The data received was statistically processed with Office Excel (Microsoft, USA). In cows with persistent corpus luteum high progesterone level (7.6±1.2 ng/ml) was observed while estradiol concentration was low (2.3±0.5 pg/ml) compared to the hormone levels in cows with available corpus luteum during normal ovarian cycle (P<0.01 and P<0.05, respectively). In cows with follicular cysts the concentration of progesterone in blood was 1.5±0.5 ng/ml, which was 5–8 times less compared to the result for luteal cysts. At the same time, the concentration of estradiol was the highest (26.3±4.0 pg/ml), which was 7.7–11.4 times higher than in cows with persistent corpus luteum and luteal cysts. Compared to the animals with follicles on the ovaries, the level of estradiol was significantly higher (P<0.01). The lowest concentrations of steroid hormones were detected in cows with ovarian hypofunction. The difference in progesterone level was significant in the group of cows with corpus luteum of the sexual cycle (P<0.001), while the difference in estradiol level was significant in the group of animals with available corpus luteum (P<0.001) and follicle (P<0.001). In cows with ovarian hypofunction the lowest level of the studied parameters was observed which were significantly different from the cows with corpus luteum of sexual cycle for diene conjugates (P<0.001), superoxide dismutase (P<0.05), glutathione peroxidase (P<0.01), hemoglobin (P<0.05), alanine aminotransferase (P<0.01) and aspartate aminotransferase (P<0.05). Comparing the group of cows with follicles in the ovaries, there were observed significant differences in the group of cows with hypofunction of the ovaries according to the indices such as diene conjugates (P<0.001), glutathione peroxidase (P<0.001), hemoglobin (P<0.05), alanine aminotransferase (P<0.01). When the follicular cysts in cows were present, the level of diene conjugates (P<0.05) and glutathione peroxidase (P<0.01) were significantly lower than in cows with follicles that developed during the normal sexual cycle. Changes of cow’s blood biochemical parameters in normal and pathological conditions of the ovaries (corpus luteum, follicle, persistent corpus luteum, hypofunction, follicular and luteal cysts) were established. In order to make a clear diagnosis of the functional state of the cow ovaries, complex examination should be carried out that would include rectal and ultrasound examination of the cows together with determination of steroid hormones and individual blood biochemical parameters.

Keywords: COWS, OVARIES, BLOOD, SEXUAL CYCLE, STEROID HORMONES

1. Bove S. E., Petroff M. G., Nishibori M., Pate J. L. Macrophage migration inhibitory factor in the bovine corpus luteum. Biology of reproduction, 2000, vol. 62, issue 4, pp. 879–885. DOI: 10.1095/biolreprod62.4.879.
2. Chomaev A. M. Diagnosis and treatment of cow follicular cysts. Reports of RAAS, 1997, vol. 4, pp. 36–37. (in Russian)
3. Dulger H. P. Cystic pathology of the ovaries in cows and the improvement of the methods for its differential diagnosis and therapy. Doctoral thesis, Moscow, 2008, 232 p. (in Russian)
4. Glaz A. V. Features of the functional disorders of the ovaries in cows. Academic records of Grodno Agrarian Institute, 1994, issue 4, 106 p.
5. Mahotkin A. H. The use of hormonal drugs for some functional disorders of the ovaries in cows. Improving the breeding and productive qualities of animals. Interuniversity collection of scientific papers, Kazan, 1995, pp. 95–99. (in Russian)
6. Murphy B. D. Models of luteinization. Biology of reproduction, 2000, vol. 63, issue 1, pp. 2–11. DOI: 10.1095/biolreprod63.1.2.
7. Nikulin A. V. Correction of metabolic and trophic processes in cows with ovarian dysfunction by biological placenta bio-normalizers. Doctoral thesis, Belgorod, 2005, 179 p. (in Russian)
8. Polancev N. I., Podberezny V. V. Veterinary obstetrics and biotechnology animal reproduction. Rostov-On-Don, Fenix, 2001, pp. 445–447. (in Russian)
9. Sharipov A. F. Functional morphology of corpus luteum ovarian cows in normal state and with pathologies. Doctoral thesis, Ufa, 2008, 158 p. (in Russian)
10. Vlizlo V. V. (ed.), Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
11. Zemlanikin V. V. Correction of reproductive function in cows with follicular ovarian cysts. Doctoral thesis, Saratov, 2004, 170 p. (in Russian)
12. Zöldág L., Vetési F., Solti L., Molnár L. A ciklikus sárgatest és a folliculus-luteincysta összehasonlító endokrinológiai és morfológiai tanulmányozása szarvasmarhában. Magyar Allatorvosok Lapja, 1986, vol. 41, issue 6, pp. 343–347. (in Hungarian)
13. Zvereva H. V., Homyn S. P. Gynecological diseases of cows. Kyiv, Urozhay, 1976, 152 p. (in Ukrainian)

Download full text in PDF