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Bìol. Tvarin. 2023; 25 (1): 20–26.
https://doi.org/10.15407/animbiol25.01.020
Received 03.10.2022 ▪ Revision 27.12.2022 ▪ Accepted 12.03.2023 ▪ Published online 31.03.2023

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Lipid composition and peroxidation products in the body tissues in bees under the action of different doses of nanotechnological Ge citrate and the probiotic Lactobacillus casei В-7280

A. Z. Pylypets¹, M. Ya. Spivak², R. S. Fedoruk¹, M. M. Tsap¹, I. I. Kovalchuk^1,3, M. M. Romanovyсh¹

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¹Institute of Animal Biology NAAS, 38 V. Stusa str., Lviv, 79034, Ukraine
²Zabolotny Institute of Microbiology and Virology NAS of Ukraine, 154 Aсad. Zabolotny Str., Kyiv, 03143, Ukraine
³Stepan Gzhytsky National University of Veterinary Medicine and Biotechnologies Lviv, 50 Pekarska str., Lviv, 79010, Ukraine

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Modern methods of preserving honey bees focus on increasing their viability, feeding level and productivity development during different growth periods. There is a tendency to use new effective remedies of natural origin. Their action mechanisms differ from synthetic substances and drugs due to the activation of the body’s protective reactions at the physiological level. The probiotic Lactobacillus casei IMV B-7280 has an effective stimulating effect on physiological processes in bees under various environmental and experimental conditions. The physiological effect of this probiotic is associated with normalizing of the intestinal bacterial microflora and participation in modulating body’s protective reactions. The resistance of honey bees also depends on mineral nutrition, which affects metabolic processes at the tissue, organ and system levels and determines the vitality and resistance of the organism. Mineral elements are involved in protein, lipid, carbohydrate and energy metabolism, they activate enzyme systems. Therefore, the aim of the research was to determine the effect of the probiotic L. сasei B-7280 in combination with different doses of Ge nanotechnological citrate (NTC) on the lipid composition and peroxidation products content in bees’ tissues. Bees of the control group were fed 60% sugar syrup (SS) in the amount of 1 cm³/group/day. The bees of the first experimental group (R1) were fed 1 cm³ of SS, 0.1 μg of Ge NTC and a solution of probiotic L. casei B-7280 (10⁶ cells/cm³); the second experimental group (R2) received 0.2 μg of Ge citrate and probiotic L. casei B-7280 (10⁶ cells/cm³). The feeding duration was 34 days. It was established that feeding sugar syrup, L. сasei and different doses of Ge citrate increased the content of phospholipids, mono- and diacylglycerols in the R2 group, cholesterol esterification in the R1 group, and decreased free cholesterol, non-esterified fatty acids compared to the K group and P period. The results indicate a dose-dependent effect of Ge NTC on lipid metabolism in body tissue homogenates. There was an increase in the triacylglycerols level in the lipids in all groups during the experimental period. The biological effect of nanotechnological Ge citrate and L. сasei cause a decrease of lipid peroxidation processes in bees of the experimental groups.

Key words: bees, Ge citrate, probiotic, lipids, peroxidation products

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