UDC 636.2:618.591.11 http://dx.doi.org/10.15407/animbiol18.04.086
INFLUENCE OF DRUG “ANTYMAST” ON THE T- AND B-CELLS LINKS OF THE IMMUNITY SYSTEM OF COWS WITH SUBCLINICAL FORM OF MASTITIS
G. V. Sobko
sobko2312@gmail.com
Institute of animal biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine
The results of experimental research of the influence of drug “Antymast” made on the basis of natural bee raw on indexes of T- and B- cells link of immunity in the blood of cows with subclinical mastitis are presented in the article.
The study was conducted in the spring period on two groups of black and white dairy cows (2nd–3rd lactation) with 5–7 animals in a group. The cows were previously diagnosed by rapid method of counting somatic cells for subclinical forms of mastitis: control group contained clinically healthy animals and research group consisted of cows with signs of subclinical mastitis. Cows in research groups were administered one injection of tube (13ml) of the drug “Antymast” in the affected udder quarters three times with intervals of 24 hours and prophylactically a half therapeutic dose in healthy udder quarters. Blood for immunological studies was taken on the 1st day (before drug administration), on the 3rd and 9th day after its application.
It has been stated that in cows suffering from subclinical form of mastitis cellular link of immune system increased (P<0.05–0.01) the absolute number of leukocytes and decreased (P<0.05–0.01) the relative amount functional activity of T-lymphocytes and their subpopulations by regulatory receptor system of redistribution of immune cells.
Introduction of research drug “Antymast” helped to reduce the number of leukocytes in the blood. On the 9th day after the injection number of leukocytes in the blood decreased (P<0.05) and was at the same level as in animals of control group. In the blood of cows of research group at the 3rd day after the injection the increasing of the total number of T- and B- lymphocytes compared to the values of this index in clinically healthy animals was observed. The total number of T-lymphocytes (active and theophylline-resistant) and B-lymphocytes in the blood of cows of research group at the 9th day after the injection was higher (P<0.05–0.01) than in the control group. Thus, the number of Th-RUL RUL-EAS and low density of receptors in the blood of cows of experimental group was greater (P<0.01), and “zero” functionally undifferentiated cells was lower (P<0.01) compared to the control.
Keywords: CATTLE, BLOOD, SUBCLINICAL MASTITIS, WHITE BLOOD CELLS, T- AND B-CELLS, SOMATIC CELLS
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