The Animal Biology

HAEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF THE F₂ RATS’ ORGANISM IN A PERIOD OF PROLONGED WATERING OF NANO-Ge CITRATE

R.S. Fedoruk¹, U. I. Tesarivska², M. I. Khrabko¹, M. M. Tsap¹, O. P. Dolaychuk¹, S. I. Kropyvka³

This email address is being protected from spambots. You need JavaScript enabled to view it.

¹Istitute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

²State Scientific-Research Control Institute of Veterinary Medical Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

³Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

In the article the results of experimental studies of the impact of different tenfold (20 and 200 μg Ge) doses of Ge citrate, obtained by nanotechnology method, on hematological and biochemical profile of the organism of the F₂ male rats of second offspring are shown.

It was established that the use of nano-Ge citrate at doses 20 and 200 μg Ge/kg of body weight with drinking water during four months leads to decrease of red blood cell number in both groups of males and to decrease platelets only under action of 20 μg of Ge, with increase of monocytes in animals of this group compared to the control. The possibility of strengthening the deponation functions of the rat organism for erythrocytes and leukocytes under the actions of the Ge citrate were discussed. In the blood of males in the research groups an increase of the albumin, Ca, P, TAG were noted that indicates a stimulatory effect of Ge citrate on its transport function and supplying structural and energy elements for organs and systems. Under the action of nano-Ge citrate in the blood of males in research groups an influence of enzymatic link of the antioxidant support network grows with the increase of SOD and HP-activity and decrease of lipid peroxidation intensity. The applied doses of nano-Ge citrate promote an increase of immunophysiological reactivity of the males’ organisms with the increase of content of middle mass molecules, circulating immune complexes, sialic acid and ceruloplasmin in their blood compared to the concentration of these components in the blood of animals in the control group.

Keywords: HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS, GLYCOPROTEINS, RATS, DOSES OF NANO-Ge CITRATE, BLOOD, WATERING

1. Asai K., Tomizawa S., Sato R. Effects of organic germanium compound on spontaneously hypertensive rats. Rep. Asai Germanium Res. Inst., 1972, no. 1, pp. 21–23.
2. Borisevich V. B., Kaplunenko V. G., Kosinov N. V. Nanomaterials in biology. Fundamentals of nanoveterinary. Kyiv, WA “Avicenna”, 2010, 416 p. (in Ukrainian).
3. Byts H.O. Prevention of gastroenteritis in calves with using medications of selenium and germanium. Scientific Herald of LNAVM named after S. Z. Gzhytsky, 2010. vol. 12, no. 3 (1), pp. 3–6. (in Ukrainian)
4. Dolaychuk O. P., Fedoruk R. S., Kovalchuk I. I., Kropyvka S. I. Physiological and biochemical processes in the organisms of rats when feeding them with different amounts of germanium citrate. The Animal Biology, 2015, vol. 17, no. 2, pp. 50–56. (in Ukrainian) https://doi.org/10.15407/animbiol17.02.050
5. Dolaychuk O. P., Fedoruk R. S., Kropyvka S. Y. Physiological reactivity and antioxidant defense system of the animal organism induced by Germanium, Chromium, and Selenium “nanoaquacitrates”. Agricultural Science and Practice, 2015, vol. 2 (2), pp. 50–55. (in Ukrainian) https://doi.org/10.15407/agrisp2.02.050
6. European convention for the protection of vertebrate animals used for experiment and other scientific purposes. Coun. of Europe, Strasbourg, 1986, 53 p.
7. Fedoruk R. S., Khrabko M. I., Dolaychuk O. P., Tsap M. M. The growth and development of the F₁ rats when watering them, and female-mothers of different doses of germanium citrate. Collection of works of Scientific symposium with international participation “Zootechnycal science — an important factor for the European type of the agriculture”, Moldova, Maximovca, 2016, pp. 780–785.
8. Fedoruk R. S., Khrabko M. I., Tsap M. M., Martsynko O. E. Growth, development and reproductive function of female rats and their offspring viability at the conditions of the watering of different doses of citrate germanium. The Animal biology, 2016, vol. 18, no. 3, pp. 97–106. (in Ukrainian)
9. Godovan V. V., Kresyun V. I., Seifullin I. J., Voloshenko B. A. New biologically active substances on the basis of germanium. Clinical Pharmacy, 2000, vol. 4, no. 4, pp. 66–67. (in Ukrainian)
10. Hunchak O. V, Kaplunenko V. G. Effect of germanium supplements in feed for productive as geese reared for meat. Manufacturing and processing of livestock products, 2015, vol. 1, pp. 156–159. (in Ukrainian)
11. Khrabko M. I., Fedoruk R. S. Growth and development of F₁ male rats’ organism and its immunophysiological activity during the period watering them different doses of nanotechnology and chemically synthesized germanium citrate. Bulletin of Kyiv National Taras Shevchenko University, Problems of regulation of physiological functions, 2016, vol. 2, no. 21, pp. 39–43. (in Ukrainian)
12. Kovalenko L. V. Evaluation of the stimulating effect of nanoaquachelates of germanium on the natural resistance of animals. Scientific Herald of NULES of Ukraine, Series: Veterinary medicine, quality and safety of products of stock-raising, 2012, vol. 172, no. 1, pp. 203–209. (in Ukrainian)
13. Kovalchuk I. I. Heavy metals and lipids in the tissue and bee production at traditional and organic bee-keeping conditions and the ways of their levels` correction. Autoref. of PhD thesis in vet. science. Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky, Lviv, 2015, 41 p. (in Ukrainian)
14. Kresyun V. I., Shemonayeva K. F., Vidavska A. G. Pharmacological characterization of compounds of germanium. Clinical Pharmacy, 2004, no. 4, pp. 65–68. (in Ukrainian)
15. Kuldonashvili K. V., Sheremeta V. I., Kaplunenko V. G. Nanoacqualate of germanium effect on the growth of piglets during the prenatal period. Animal Breeding and Genetic, 2016, vol. 51, pp. 261–266. (in Ukrainian)
16. Lukevics E. J., Gard T. K., Ignatovich L. M., Mironov V. F. The biological activity of compounds of germanium. Riga, Zinatne, 1990, 191 p.
17. Lukyanchuk V. D., Nemyatyh O. D. Influence of coordination compounds of germanium with nicotinic acid on the activity of enzymes of energy obmela under extreme oxygen-deficient state. Ukrainian Journal of Extreme Medicine named after G. O. Mozhaev, 2003, no. 1, pp. 62–66. (in Ukrainian)
18. Sahanda I. V. Preparations germanium and their use in medicine. Ukrainian Scientific Medical Youth Journal, 2014, vol. 4, no. 84, pp. 83–86. (in Ukrainian)
19. Shatorna V. F., Garets V.  I., Savenkova O. O., Kolosova I. I. The influence of heavy metals and nanometals to the condition of reproductive function in experiment. Tavria Medical and Biological Messenger, 2013, vol. 16, no. 1, pp. 246–250. (in Ukrainian)
20. Stadnik A. M, Byts G. A, Stadnyk O. A. The biological role of germanium in animals and humans. Scientific Messenger of LNAVM named after S. Z. Gzhytsky, 2006, vol. 2, no. 1, pp. 174–85. (in Ukrainian)
21. Tesarivska U. I., Fedoruk R. S., Shumska M. I. Reproductive function of rat females and postnatal development of F₁ and F₂ offspring for the actions of different doses of nanogermanium citrate. Scientific Messenger of LNUVMBT named after S. Z. Gzhytsky, 2016, vol. 18, no. 3 (71), pp. 124–129. (in Ukrainian)
22. Thayer J. S. Germanium compounds in biological systems. Rev. Silicon, Germanium, Tin, Lead Compd., 1985, vol. 8, no. 2–3, pp. 133–155.
23. Ukraine patent for utility model number 38391. IPC (2006): C07C 51/41, C07F 5/00, C07F 15/00, C07C 53/126 (2008.01), C07C 53/10 (2008.01), A23L 1/00, B82B 3/00. Method metal carboxylates “Nanotechnology receiving metal carboxylates”. Kosinov M. V., Kaplunenko V. G. Publish. 12.01.2009, Bull. no. 1. (in Ukrainian)
24. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. A reference book. Ed. by V. V. Vlizlo. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
25. Xie W., Chen X., Yang K. Effects of selenium and germanium on lipid peroxidation in rats fed with low selenium grain. Zonghua Yu Fang Yi Xue Za Zhi, 1996, vol. 30, no. 2, pp. 88–90.

Download full text in PDF format