The Animal Biology

THE EFFECT OF SILICON COMPOUNDS ON IMMUNOPHYSIOLOGICAL REACTIVITY OF RABBITS

А. I. Ivanytska, Ya. V. Lesyk, M. M. Tsap

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article displays the results of watering different amounts of silicon citrate obtained by nanotechnology and sodium metasilicate during the 52^nd–110^th days of life on indicators of cellular and humoral immunity of rabbits. The significant difference of phagocytic activity of neutrophils in the blood of rabbits receiving silicon citrate was found. This indicates stimulating effect of the silicon organic compound on nonspecific resistance cell link. Watering silicon citrate to the animals in research groups compared to watering sodium metasilicate and control had more significant effect on content of lysozyme activity and blood serum bactericidal activity as integral factors of nonspecific resistance of humoral type which was affected by the increase (P<0.05–0.01) of their content in blood on the 31^st and the 58^th day of the research.

The use of organic additives in rabbits’ diets had a stimulating effect of their immune system which was indicated by higher (P<0.05–0.01) content of hexoses associated with proteins, sialic acid and ceruloplasmin in blood of animals which were watered silicon citrate during the research while watering the sodium metasilicate in smaller amount helped to increase (P<0.05) the content of hexoses associated with proteins in rabbits’ blood on the first stage of research and to increase the sialic acid on the last stage compared to the control.

The addition of silicon citrate to the rations caused the significant increase of immunoglobulin level in rabbits’ blood during the research and the CIC concentration on the first stage of experiment compared to control which indicates the activating effect of silicon citrate on synthesis of some of their classes in the lymphatic system which provides mechanisms for both humoral and cellular immunity.

Keywords: RABBITS, IMMUNOPHYSIOLOGICAL REACTIVITY, SILICON AQUANANOCITRATE, SODIUM METASILICATE, RESISTANCE, IMMUNOGLOBULINS, GLYCOPROTEINS

1. Borysevych V. B., Kaplunenko V. G., Kosinov M. V. Nanomaterials in biology. Fundamentals of nanoveterinary. A textbook for veterinary students and for veterinary and medical specialists. Kyiv, publishing house “Avicenna”, 2010, 416 p. (in Ukrainian)
2. Bakalyuk O. Y., Panchyshyn N. Ya., Dzyga S. V. The endogenous intoxication syndrome, mechanism of its development, identification methods. Bulletin of scientific researches, 2000, no. 1, pp. 11–13. (in Ukrainian)
3. De Blas C., Wiseman J. Nutrition of the Rabbit. 2^nd Edition. Library of Congress Cataloging-in-Publication Data, 2010, 325 p. https://doi.org/10.1079/9781845936693.0000
4. Chekman I. S., Ulberg Z. R., Malanchuk V. O. Nanoscience, nanobiology, nanopharmaceutics. Kyiv, Poligraphplus, 2012, 328 p. (in Ukrainian)
5. Eliseev A. A., Lukashin A. V. Functional Nanomaterials. Moscow, Fizmatlit, 2010, 456 p. (in Russian)
6. Fortina P., Kricka L. J., Surrey S. Nanobiotechnology the promise and reality of new approaches to molecular recognition. Trends Biotechnol., 2005, vol. 23, no. 5, pp. 168–173. https://doi.org/10.1016/j.tibtech.2005.02.007
7. Gerasimov I. G. Functional heterogeneity of neutrophils. Clinical laboratory diagnostics, 2006, no. 2, pp. 34–36. (in Russian)
8. Galochkin V. A., Cherepanov G. G. Non-specific resistance of farm animals: difficulties of identification, problems, solutions. Problems of productive animal biology, 2013, no. 1, pp. 5–29. (in Russian)
9. Huntoon K. M., Wang Y., Eppolito C. A., Barbour K. W., Berger F. G., Shrikant P. A., Baumann H. The acute phase protein haptoglobin regulates host immunity. J. Leukocyte Biol., 2008, vol. 84 (1), pp. 170–181. https://doi.org/10.1189/jlb.0208100
10. Klitsenko H. T., Kulyk M. F., Kosenko M. V., Lisovenko V. T. Mineral nutrition of animals. Kyiv, Svit, 2001, p. 575. (in Ukrainian)
11. Knopp D., Tang D., Neissner R. Bioanalytical applications of biomolecule-functionalized nanometer-sized doped silica particles: a review. Analitica Chimica Acta, 2009, no. 64, pp. 14–30.
12. Lowe J. B. Glycosylation, immunity and autoimmunity. Cell, 2001, vol. 104 (6), pp. 809–812. https://doi.org/10.1016/S0092-8674(01)00277-X
13. Lesyk Ya. V. Resistance of the rabbit dams’ organism at drinking supplemuted by chlorella suspension, sodium sulfate, chromium chloride and citrate. The Animal Biology, 2013, vol. 15 (2), pp. 90–96. (in Ukrainian)
14. Murthy S. K. Nanoparcticles in modern medicine: state of the art and future challenges. Int. J. Nanomed., 2007, vol. 2, pp. 129–141.
15. Martin K. R. The chemistry of silica and its potential health benefits. Journal of Nutrition Health & Aging, 2007, pp. 94–98.
16. Na M., Park H., Ahn M. Synthesis of organic-inorganic hybrid sols with nanosalica particles and organoalkokysilanes for transparent and high-thermal-resistance coating films using solgel reaction. J. Nanosci. Nanotechnol., 2010, vol.10, no. 10, pp. 6992–6995. https://doi.org/10.1166/jnn.2010.2988
17. Official Journal of the European Union L276/33, 2010. Directive 2010/63/EU of The European Parliament and of The Council of 22 September 2010 on the protection of animals used for scientific purposes. 86/609/EC. 20.10.2010.
18. Price C. T., Koval K. J., Langford J. R. Silicon: A review of its potential role in the prevention and treatment of postmenopausal osteoporosis. Hindawi Publishing Corporation International Journal of Endocrinology, vol. 2013, pp. 1–6.
19. Plehova N. G. Bactericidal activity of phagocytes. Journal of Epidemiology, Microbiology and Immunobiology, 2006, no. 6, pp. 89–96. (in Russian)
20. Russell R. F., McDonald J. U., Lambert L., Tregoning J. S. Use of the microparticle nanoscale silicon dioxide as an adjuvant to boost vaccine immune responses against influenza virus in neonatal mice. Journal of Virology, 2016, vol. 90, no, 9, pp. 4735–4744. https://doi.org/10.1128/JVI.03159-15
21. Skalny A. V., Rudakov I. A. Bioelements in medicine. “Onyx 21” Publishing House, World, 2004, 272 p. (in Russian)
22. Se-Yune Kim, Sang-Ki Kim, Mi-Kyeong Choi. Effect of Silicon Supplementation on Immune Response in Male and Female Mice. The FASEB Journal, 2016, vol. 30, no., 4 pp. 34–40.
23. Semenova Yu. V. Resistance and productivity in pigs when using a silicon-containing drug in rations. Modern problems of intensification of pork production in the CIS countries, Ulyanovsk State Agricultural Academy, 2010, vol. 1, pp. 247–252. (in Russian)
24. Smitha S., Shojesh P., Mukundan P. Synthesis of biocompatible hydrophobic silica-gelatin nanohybrid by sol-gel process. Colloids Surf. B. Biointerfaces, 2007, vol. 55, no. 1, pp. 38–43. https://doi.org/10.1016/j.colsurfb.2006.11.008
25. Serdiuk A. M., Gulich M. P., Kaplunenko V. G., Kosinov M. V. Nanotechnology of micronutrients: problems, perspectives and ways to eliminate the deficit of macro- and microelements. Bulletin of Academy of medical sciences, 2010, no. 1, pp. 47–53. (in Ukrainian)
26. Sato C. Chain length diversity of sialic acids and its biological significance trend singly coscience and glycotechnology. Trends in Glycoscience and Glycotechnology, 2004, vol. 16, no. 91, pp. 331–344. https://doi.org/10.4052/tigg.16.331
27. Trachtenberg I. M., Chekman I. S., Linnik V. O., Kaplunenko V. G. Interaction micronutrients, biological, medical and socialaspects. Bulletin of the National Academy of Sciences, 2013, vol. 6, pp. 11–20. (in Ukrainian)
28. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of investigation in biology, stock-breeding and veterinary. Lviv, 2012, 764 p. (in Ukrainian)

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