Bìol. Tvarin, 2018, volume 20, issue 4, pp. 61–68


O. O. Sushko1,2, R. Ya. Iskra1, V. I. Pryimych2

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1Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

2Lviv National University of the Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50, Pekarska str, Lviv 79010, Ukraine

We have investigated the activity of the antioxidant system and the level of peroxide oxidation of lipids in the liver of rats with aloxane-induced diabetes and influence of chromium citrate, which was given to animals during one month in an amount of 0.1 and 0.2 mg/ml of water. Diabetes mellitus was experimentally induced by intraperitoneal injection of 5 % solution of alloxan monohydrate in an amount of 150 mg/kg body weight. Diabetes was detected by measuring glucose levels in blood collected from the tail vein. On the 40th day of the experiment, after the decapitation of the animals, the liver tissue for research was collected.

Content of lipid hydroperoxides and TBA-active products significantly increased in the rat liver homogenate of the control diabetic group. Content of lipid hydroperoxides and TBA-active products decreased in the liver of rats watered by chromium citrate in the amount of 0.1 mg/ml compared to corresponding levels in the diabetic control group.

The activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and the content of reduced glutathione significantly decreased in the liver of animals of the control diabetic group. However, under the effect of chromium citrate in the amount of 0.1 and 0.2 mg/ml of water those indications normalized, namely, the activity of catalase, superoxide dismutase, glutathione peroxidase increased and the content of reduced glutathione increased, compared to their levels in the liver of animals in the diabetic control group.

Probable changes in the content of products of lipid peroxidation and the activity of enzymes in the antioxidant system in the liver are most noticeable when using chromium citrate in the amount of 0.1 mg/ml of water. These indexes show the normalization of antioxidant protection for the effects of chromium citrate in tissues of rats with experimentally induced diabetes.


  1. Abou-Seif M. A, Youssef A. A. Evaluation of some biochemical changes in diabetic patients. Clinica Chimica Acta, 2004, vol. 346, issue 2, pp. 161–170. https://doi.org/10.1016/j.cccn.2004.03.030
  2. Ahmadvand H., Dehnoo M. G., Cheraghi R., Rasoulian B., Ezatpour B., Azadpour M., Baharvand K. Amelioration of altered serum, liver, and kidney antioxidant enzymes activities by sodium selenite in alloxan-induced diabetic rats. Reports of Biochemistry and Molecular Biology, 2014, vol. 3, no. 1, pp. 4–20.
  3. Chevari S., Andyal T., Shtirenger D. Determination of the antioxidant properties of blood and their diagnostic value in old age. Laboratory Work, 1991, vol. 10, pp. 9–13. (in Russian)
  4. Davies S., McLaren Howard J., Hunnisett A., Howard M. Age-related decreases in chromium levels in 51 665 hair, sweat, and serum samples from 40 872 patients: Implications for the prevention of cardiovascular disease and type II diabetes mellitus. Metabolism, 1997, vol. 46, issue 5, pp. 469–473. https://doi.org/10.1016/S0026-0495(97)90179-7
  5. Elsner M., Tiedge M., Guldbakke B., Munday R., Lenzen S. Importance of the GLUT2 glucose transporter for pancreatic beta cell toxicity of alloxan. Diabetologia, 2002, vol. 45, issue 11, pp. 1542–1549. https://doi.org/10.1007/s00125-002-0955-x
  6. Fairweather D., Rose N. R. Type 1 Diabetes: virus infection or autoimmune disease. Nature Immunology, 2002, vol. 3, pp. 338–340. https://doi.org/10.1038/ni0402-338
  7. Iskra R. Ya. Antioxidant system in the body by the action of rabbit chromium compounds. Studia Biologica, 2012, vol. 6, no. 1, pp. 77–86. (in Ukrainian) https://doi.org/10.30970/sbi.0601.201
  8. Iskra R. Ya, Vlizlo V. V. Peculiarities of antioxidant defense system in erythroid cells and tissues of pigs under action of chromium chloride. The Ukrainian Biochemical Journal, 2012, vol. 85, issue 3, pp. 96–102. (in Ukrainian) https://doi.org/10.15407/ubj85.03.096
  9. Jeejeebhoy K. N., Chu R. C., Marliss E. B., Greenberg G. R., Bruce-Robertson A. Chromium deficiency, glucose intolerance, and neuropathy reversed by chromium supplementation, in a patient receiving long-term total parenteral nutrition. The American Journal of Clinical Nutrition, 1977, vol. 30, issue 4, pp. 531–538. https://doi.org/10.1093/ajcn/30.4.531
  10. Korobeinikova E. N. Modification of the LPO determination in the reaction with TBA. Laboratory Work, 1989, vol. 7, pp. 8–10. (in Russian)
  11. Korolyuk M. A., Ivanova L. I., Maiorova I. G., Tokarev V. E. A method for measuring catalase activity. LaboratoryWork, 1988, vol. 1, pp. 16–19. (in Russian)
  12. Lin C. C., Huang H. H., Hu C. W., Chen B. H., Chong I. W., Chao Y. Y., Huang Y. L. Trace elements, oxidative stress and glycemic control in young people with type 1 diabetes mellitus. Journal of Trace Elements in Medicine and Biology, 2014, vol. 28, issue 1, pp. 18–22. https://doi.org/10.1016/j.jtemb.2013.11.001
  13. Lowry O. H. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 1951, vol. 193, no. 1, pp. 265–275.
  14. Lucchesi A. N., de Freitas N. T., Cassettari L. L., Marques S. F. G., Spadella C. T. Diabetes mellitus triggers oxidative stress in the liver of alloxan-treated rats: a mechanism for diabetic chronic liver disease. Acta Cirurgica Brasileira, 2013, vol. 28, no. 7, pp. 502–508. https://doi.org/10.1590/S0102-86502013000700005
  15. Lushchak O. V., Kubrak O. I., Torous I. M., Nazarchuk T. Y., Storey K. B., Lushchak V. I. Trivalent chromium induces oxidative stress in gold fish brain. Chemosphere, 2009, vol. 75, issue 1, pp. 56–62. https://doi.org/10.1016/j.chemosphere.2008.11.052
  16. Manna P., Das J., Ghosh J., Sil P.C. Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IkappaBalpha/NF-kappaB, MAPKs, and mitochondria-dependent pathways: Prophylactic role of arjunolic acid. Free Radical Biology and Medicine, 2010, vol. 48, issue 11, pp. 1465–1484. https://doi.org/10.1016/j.freeradbiomed.2010.02.025
  17. Mironchik V. V. Method of determination of lipid hydroperoxides in biological tissues. Patent SU no. 1084681, 1984. (in Russian)
  18. Mishra N., Singh N. Blood viscosity, lipid profile, and lipid peroxidation in type-1 diabetic patients with good and poor glycemic control. North American Journal of Medical Sciences, 2013, vol. 5, issue 9, pp. 562–566. https://doi.org/10.4103/1947-2714.118925
  19. Moin V. M. A simple and specific method for determining glutathione peroxidase activity in erythrocytes. Laboratory Work, 1986, vol. 12, pp. 724–727. (in Russian)
  20. Palsamy P., Sivakumar S., Subramanian S. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin-nicotinamide-induced experimental diabetic rats. Chemico-Biological Interactions, 2010, vol. 186, issue 2, pp. 200–210. https://doi.org/10.1016/j.cbi.2010.03.028
  21. Praveeena S., Pasula S., Sameera K. J. Trace elements in diabetes mellitus. Journal of Clinical and Diagnostic Research, 2013, vol. 7, issue 9, pp. 1863–1865. https://doi.org/10.7860/JCDR/2013/5464.3335
  22. Vincent J. B. Is chromium pharmacologically relevant? Journal of Trace Elements in Medicine and Biology, 2014, vol. 28, issue 4, pp. 397–405. https://doi.org/10.1016/j.jtemb.2014.06.020
  23. Vincent J. B. Quest for the molecular mechanism of chromium action and its relationship to diabetes. Nutrition Reviews, 2000, vol. 58, issue 3, pp. 67–72. https://doi.org/10.1111/j.1753-4887.2000.tb01841.x
  24. Vladimirov Yu. A., Archakov A. I. Peroxide oxidation of lipid in biological membranes. Moscow, Nauka, 1972, 252 p. (in Russian)
  25. Vlizlo V. V. (ed.), Fedoruk R. S., Ratych I. B. Laboratory methods of research in biology, veterinary medicine. A guide. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
  26. Wang N., Li T., Han P. The effect of tianmai xiaoke pian on insulin resistance through PI3-K/AKT signal pathway. Journal of Diabetes Research, 2016, Article ID 92612599261259, pp. 1–8. https://doi.org/10.1155/2016/9261259

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