Bìol. Tvarin. 2021; 23 (2): 19–25.
Received 08.04.2021 ▪ Accepted 17.05.2021 ▪ Published online 01.07.2021

Diagnostic value of integrated immunohematological indices as markers of chronic stress in laying hens

Yu. Osadcha

This email address is being protected from spambots. You need JavaScript enabled to view it.

National University of Life and Environmental Sciences of Ukraine,
15 Heroyiv Oborony str., Kyiv, 03041, Ukraine

The study of stress in the conditions of industrial technologies of keeping laying hens and determining the level of technological stressors influence on the physiological state of poultry is a necessary condition for the development of new methods of stress prevention in choosing the best ways to keep them. The aim of the study was to determine the informativeness of integrated immunohematological indices for the diagnosis of stress-induced disorders in laying hens under the influence of technological stressors of varying intensity. Chronic technological stress was modeled by long-term keeping of laying hens at high planting density. The intensity of the stressor was determined by increasing the density of laying hens. Integral immunohematological indices were determined on the basis of an extended general blood test. It has been found that in laying hens, which due to prolonged exposure to high density were in a state of chronic stress, there is a high level of endogenous intoxication and impaired immunological reactivity, as evidenced by increased Leukocyte shift index, Immunoreactivity index, Leukocyte to erythrocyte sedimentation rate ratio, lymphocyte to monocyte ratio, heterophil to monocyte ratio. It is shown that under chronic stress in laying hens there is activation of the cellular part of the immune system, active adaptive response of white blood, as well as the predominance of delayed-type reactions over immediate-type hypersensitivity, as indicated decrease in lymphocyte-granulocyte index, general index, lymphocytic index and lymphocyte to eosinophil ratio. Thus, integrated immunohematological indices are promising markers for the diagnosis of chronic stress in laying hens.

Key words: immunohematological indices, laying hens, stress, immunological reactivity, endogenous intoxication

  1. Belyaeva EY, Buslovskaya LK. Adaptive reactions and biochemical parameters of the blood of chickens under different light conditions. Sci. Bull. Belgorod State Univer., Ser. Nat. Sci. 2012; 21 (140), 21/1: 143–148. Available at: http://dspace.bsu.edu.ru/handle/123456789/18540 (in Russian)
  2. Bondarchuk IV, Sydorchuk LP, Sydorchuk IY. Adaptation stress level and cell reactivity in arterial hypertensive patients with coronary heart disease. Bukovynian Med. Bull. 2016; 20 (2/78): 16–19. DOI: 10.24061/2413-0737.XX.2.78.2016.62. (in Ukrainian)
  3. Dawkins MS. Behavior as a tool in the assessment of animal welfare. Zool. 2003; 106 (4): 383–387. DOI: 10.1078/0944-2006-00122.
  4. Derkho MA, Samoilova ES. Integral indices of intoxication as a criterion for assessing the level of endogenous intoxication in babesiosis. Sci. Notes KSAVM N. E. Bauman. 2011; 207: 170–177. (in Russian)
  5. Djuriak VS. Cellular reactivity and stress level adaptivity of patients with community acquired pneumonia. Clin. Exp. Pathol. 2015; 14 (4): 32–35. DOI: 10.24061/1727-4338.XIV.4.54.2015.8. (in Ukrainian)
  6. Fisinin VI, Papazyan T, Suray P. Innovative methods of dealing with stress in poultry. Poultry Prod. 2009; 8: 10–14. (in Russian)
  7. Flügge G. Effects of cortisol on brain alpha2-adrenoceptors: potential role in stress. Neurosci. Biobehav. Rev. 1999; 23 (7): 949–956. DOI: 10.1016/S0149-7634(99)00028-7.
  8. Gao SQ, Huang LD, Dai RJ, Chen DD, Hu WJ, Shan YF. Neutrophil-lymphocyte ratio: a controversial marker in predicting Crohn’s disease severity. Int. J. Clin. Exp. Pathol. 2015; 8 (11): 14779–14785. PMID: 26823804.
  9. Heidt T, Sager HB, Courties G, Dutta P, Iwamoto Y, Zaltsman A, von Zur Muhlen C, Bode C, Fricchione GL, Denninger J, Lin CP, Vinegoni C, Libby P, Swirski FK, Weissleder R, Nahrendorf M. Chronic variable stress activates hematopoietic stem cells. Nat. Med. 2014; 20 (7): 754–758. DOI: 10.1038/nm.3589.
  10. Kavtarashvili AS, Kolokolnikova TN. Physiology and productivity of poultry under stress (review). Agricul. Biol. 2010; 4: 25–37. Available at: http://www.agrobiology.ru/4-2010kavtarashvili.html (in Russian)
  11. Khabirov TS. The level of the reactive response of neutrophils as an indicator of the severity of endogenous intoxication in abdominal sepsis. Proc. IX SFULT Congress, Luhansk. 2002: 223. (in Russian)
  12. Kholodkovskaya VD, Barabanov AL. Using integral hematological indices to assess severity of endogenous toxicosis in chronic dermatoses. Internat. Sci. Pract. Conf. “World Sci.”. 2015; 3 (2): 69–72.
  13. Letkin AI. Leukocytal indexes of laying hen blood in nonspecific stress syndrome. Bull. Altai State Agr. Univer. 2020; 2 (184): 102–108. Available at: http://www.asau.ru/vestnik/2020/2/102-108.pdf (in Russian)
  14. Lewandowski RA. Cellular and immunological reactivity of the organism in patients after resection of the upper and lower jaws for the removal of malignant tumors. Clinical and experimental pathology. 2014; 12 (2): 83–87. (in Ukrainian)
  15. Marino L. Thinking chickens: a review of cognition, emotion, and behavior in the domestic chicken. Anim. Cognition. 2017; 20: 127–147. DOI: 10.1007/s10071-016-1064-4.
  16. Matolic UD. Diagnostic value of hematological indices in phlegmons of the maxillofacial area and neck. Sci. Bull. Uzhhorod Univer. Med. Ser. 2016; 1 (53): 108–110. (in Ukrainian)
  17. Maxwell MH, Hocking PM, Robertson GW. Differential leucocyte responses to various degrees of food restriction in broilers, turkeys and ducks. Brit. Poult. Sci. 1992; 33 (1): 177–187. DOI: 10.1080/00071669208417455.
  18. Miftakhutdinov AV. Experimental approaches to stress diagnostics in poultry (review). Agricult. Biol. 2014; 2: 20–30. DOI: 10.15389/agrobiology.2014.2.20eng. (in Russian)
  19. Mustafina ZG, Kramarenko YS, Kobtseva VY. Integral hematological parameters in the assessment of the body’s immunological reactivity in patients with ophthalmopathology. Clin. Lab. Diagnost. 1999; 5: 47–48. (in Russian)
  20. Nikiforov A. Neurology: a complete explanatory dictionary. Moscow, Eksmo. 2010; 464. (in Russian)
  21. Nwaigwe CU, Ihedioha JI, Shoyinka SV, Nwaigwe CO. Evaluation of the hematological and clinical biochemical markers of stress in broiler chickens. Vet. World. 2020; 13 (10): 2294–2300. DOI: 10.14202/vetworld.2020.2294-2300.
  22. Ostrovska LY, Moshel TM, Ivanytskiy IO. Analysis of haemograms in patients with inflammatory and inflammatory-dystrophic changes of periodontal tissue. Bull. Probl. Biol. Med. 2016; 1 (126): 360–363. Available at: https://vpbm.com.ua/ua/vpbm-2016-01-1/7565 (in Ukrainian)
  23. Ostrovsky VK. On the indicators of the norm of the leukocyte index of intoxication. Clin. Lab. Diagnost. 2003; 1: 45–46. (in Russian)
  24. Sierzega M, Lenart M, Rutkowska M, Surman M, Mytar B, Matyja A, Siedlar M, Kulig J. Preoperative neutrophil-lymphocyte and lymphocyte-monocyte ratios reflect immune cell population rearrangement in resectable pancreatic cancer. Ann. Surg. Oncol. 2017; 24 (3): 808–815. DOI: 10.1245/s10434-016-5634-0.
  25. Sipliviy VA, Kohn EV, Evtushenko DV. Application of the leukocyte indices for prognostication of peritonitis outcome. Clin. Surg. 2009; 9: 21–26. (in Russian)
  26. Tkachenko EA, Derkho MA. Leukocyte indices in experimental cadmium intoxication in mice. Proc. Orenburg State Agr. Univer. 2014; 3: 81–83. (in Russian)
  27. Radzykhovskii ML, Goralskii LP, Borissevich BV, Dyshkant OV. Integral indexes of intoxication in caninae coronaviridae enteritis. Sci. Bull. Vet. Med. 2018; 2: 13–19. DOI: 10.33245/2310-4902-2018-144-2-13-19. (in Ukrainian)
  28. Raznatovskaya EN. Integral indices of endogenous intoxication in patients with chemoresistant pulmonary tuberculosis. Curr. Iss. Pharm. Med. Sci. Pract. 2012; 2 (9): 119–120. (in Russian)
  29. Rekalova OM, Panasyukova OR, Koval NG. The use of leukocyte indices in immunological evaluation of inflammatory activity in patients with chronic obstructive pulmonary disease. Asthma Allergy. 2017; 1: 27–33. Available at: http://www.ifp.kiev.ua/doc/journals/aa/17/pdf17-1/27.pdf (in Ukrainian)
  30. Rushen J. Problems associated with the interpretation of physiological data in the assessment of animal welfare. Appl. Anim. Behaviour Sci. 1991; 28 (4): 381–386. DOI: 10.1016/0168-1591(91)90170-3.
  31. Scanes CG. Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio. Poult. Sci. 2016; 95 (9): 2208–2215. DOI: 10.3382/ps/pew137.
  32. Shabalov NP, Ivanov DO, Shabalova NN. Heterogeneity of the systemic inflammatory response in neonatal. Med. Acad. J. 2001; 1 (3): 81–90. (in Russian)
  33. Stanishevskaya OI, Fedorova ES. Comparative evaluation of the peculiarities of stress reactivity of the Russian white breed chicken with sw+ mutation and amrox in hypothermia conditions during embryonal and early postnatal periods of ontogenesis. Agricult. Biol. 2019; 54 (6): 1135–1143. DOI: 10.15389/agrobiology.2019.6.1135rus. (in Russian)
  34. Surai P, Fisinin VI. The modern anti-stress technologies in poultry: from antioxidants to vitagenes. Agricult. Biol. 2012; 4: 3–13. DOI: 10.15389/agrobiology.2012.4.3eng.
  35. Weimer SL, Wideman RF, Scanes CG, Mauromoustakos A, Christensen KD, Vizzier-Thaxton Y. An evaluation of methods for measuring stress in broiler chickens. Poult. Sci. 2018; 97 (10): 3381–3389. DOI: 10.3382/ps/pey204.
  36. Yabluchanskiy NI, Pilipenko VA, Kondratenko PH. Leukocyte shift index as a marker of the body’s reactivity in acute inflammation. Lab. Work. 1983; 1: 60–61. (in Russian)
  37. Zamaziy AA. Hemocytopoiesis of functionally active newborn calves and calves in the state of hypoxia. Theor. Appl. Vet. Med. 2018; 6 (3): 44‒49. DOI: 10.32819/2018.63009.
  38. Zubchenko SO, Akimova VM, Lapovetz LE. Detection donozological violations under change of integral hematological indices in patients with chronic Epstein-Barr virus infection in latent phase. Bull. Probl. Biol. Med. 2014; 4 (1/113): 125–128. Available at: https://vpbm.com.ua/ua/vpbm-2014-04-1/7214 (in Ukrainian)

gslogo

ICLOGO

cr

nbuv

ouci0

WorldCat Logo

oa

Search