The Animal Biology

Endogenic retroviruses of PERV A/C in genome of Ukrainian pigs and their correlation with the level of fat in carcasses

T. M. Ryk

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Andrei Krupynskyi Lviv Medical Academy,
70 Petro Doroshenko str., Lviv, 79000, Ukraine

The article presents the analysis of the PERV retrovirus subtypes A and C frequency in populations of Ukrainian and foreign breed pigs. Different frequencies of the PERV A/C genome presence in animals of the studied breeds were established. The largest relative number was observed in the group of wild pigs (86%), the smallest was in the groups of Poltava meat and Piétrain breeds. Animals free of both virus subtypes were found in all study groups. The article considers the hypothesis of an increase in the frequency of PERV retrovirus in the pigs’ genome during domestication. Its integration caused a gene mutation responsible for fat deposition which led to increased fat amount in carcasses and could be picked up by selection in the process of creating breeds. However, there is no obvious link between the spread of the virus in modern breeds in different areas of productivity. Also, there is no association between carcass fat amount and the presence of PERV in the genome. It is established that the information on the PERV A/C distribution in pig breeds hold in Ukraine is useful in terms of the possibility of using each of them for xenotransplantation. Also, this information can be used to justify the selection of founding breeds in order to create lines of pigs free from the endogenous retrovirus genome.

Key words: DNA typing, xenotransplantation, endogenous PERV retroviruses of types A and C, fat thickness, pig breeds

1. Guo F, Xing X, Hawthorne WJ, Dong Q, Ye B, Zhang J, Liang Q, Nie W, Wang W. Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China. Virol. J. 2014; 11: 212. DOI: 10.1186/s12985-014-0212-1.
2. Harrison I, Takeuchi Y, Bartosch B, Stoye JP. Determinants of high titer in recombinant porcine endogenous retroviruses. J. Virol. 2004; 78 (24): 13871–13879. DOI: 10.1128/JVI.78.24.13871-13879.2004.
3. Hering BJ, Cooper DK, Cozzi E, Schuurman HJ, Korbutt GS, Denner J, O’Connell PJ, Vanderpool HY, Pierson RN. The international xenotransplantation association consensus statement on conditions for undertaking clinical trials of porcine islet products in type 1 diabetes-executive summary. Xenotransplantation. 2009; 16 (4): 196–202. DOI: 10.1111/j.1399-3089.2009.00547.x.
4. Hlazko VY, Shulha EV, Dyman TN, Hlazko HV. DNA technologies and bioinformatics in solving the problems of mammalian biotechnology. Bila Tserkva, 2001: 488 p. (in Russian)
5. Kimsa MC, Strzalka-Mrozik B, Kimsa MW, Gola J, Nicholson P, Lopata K, Mazurek U. Porcine endogenous retroviruses in xenotransplantation — molecular aspects. Viruses. 2014; 6 (5): 2062–2083. DOI: 10.3390/v6052062.
6. Lin CL, Ponsuksili S, Tholen E, Jennen DG, Schellander K, Wimmers K. Candidate gene markers for sperm quality and fertility of boar. Anim. Reprod. Sci. 2006; 92 (3–4): 349–363. DOI: 10.1016/j.anireprosci.2005.05.023.
7. Nikitin SV, Yudin NS, Knyazev SP, Aitnazarov RB, Bekenev VA, Deeva VS, Goncharenko GM, Kobzev VF, Savina MA, Ermolaev VI. Differentiation of wild boar and domestic pig populations based on the frequency of chromosomes carrying endogenous retroviruses. Nat. Sci. 2010; 2 (6): 527–534. DOI: 10.4236/ns.2010.26066.
8. Nikitin SV, Yudin NS, Knyazev VA, Aytnazarov RB, Kobzev VF, Bekenev VA, Savina MA, Yermolaev VI. Frequency of chromosomes carrying endogenous retroviruses in the populations of domestic pig and wild boar. Russ. J. Gen. 2008; 44 (6): 686–693. DOI: 10.1134/S1022795408060082. (in Russian)
9. Walsh PS, Metzger DA, Higuchi R. Chelex-100 as a medium for extraction of DNA for PCR-based typing from forensic material. BioTechniques. 1991; 10 (4): 506–513. Available at: https://www.future-science.com/doi/10.2144/000114018
10. Yudyn NS, Aitnazarov RB, Ermolaev VY. Endogenous retrovirus in pigs: how high is the risk of infection with xenotransplantation? Vavilov J. Gen. Breed. 2011; 15 (2): 340–350. Available at: http://www.bionet.nsc.ru/vogis/pict_pdf/2011/15_2/13.pdf (in Russian)