FUNCTIONAL AND STRUCTURAL STATE OF RATS KIDNEYS AND LIVER UNDER THE INFLUENCE OF NANO-POLYMER BASED ON PSEUDOPOLYAMINO ACIDS

Bìol. Tvarin, 2016, Volume 18, Issue 3, pp. 107–113

http://dx.doi.org/10.15407/animbiol18.03.107

FUNCTIONAL AND STRUCTURAL STATE OF RATS KIDNEYS AND LIVER UNDER THE INFLUENCE OF NANO-POLYMER BASED ON PSEUDOPOLYAMINO ACIDS

B. O. Chekh¹, M. V. Ferens², Y. V. Martyn¹, D. D. Ostapiv¹, V. V. Vlizlo¹

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¹Institute of Animal Biology NAAS,
38 Vasyl Stus st., Lviv 79034, Ukraine

²National University “Lviv Polytechnic”,
department of Organic Chemistry,
2 St. Yura square, Lviv 79013, Ukraine

The article presents results of research of the influence of nano-polymer based on pseudopolyamino acids named GluLa-DPG-PEG600 on structural and functional state of kidneys and liver of Rattus norvegicus var. alba. This article precisely describes the influence of GluLa-DPG-PEG600 on activity of alanine transaminase (ALAT), aspartate transaminase (ASAT), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGTP) and content of cholesterol and creatinine in blood, — moreover histological analysis of rats liver and kidneys was prepared.

We found that single injection of GluLa-DPG-PEG600 caused increasing activity of ALAT, ASAT, ALP, GGTP and amount of creatinine in blood of rats on the 7^th day of experiment. We observed decreased level of enzymes and content of cholesterol and creatinine on the 14^th day of experiment comparing with results obtained on the 7^th day of experiment. On the 21^st day of experiment activity of all enzymes and content of creatinine and cholesterol returned to the same level of activity as in the control group animals.Histological analysis revealed few inflammation processes in ascending convoluted tubules of nephrons on the 7^th and 14^th days of experiment that confirms increased enzyme activity and content of creatinine and cholesterol. In deed on the 21^st day of experiment histological structure of rats and liver were unchanged.

Our results show temporary little toxic effect of GluLa-DPG-PEG600 on rats on the 7^th and 14^th days of experiment. Decreased toxic effect on the 14^th day and its absence on 21^st day of experiment can be explained by adaptation process to repeated injections of GluLa-DPG-PEG600 in rats. In conclusion GluLa-DPG-PEG600 could be used as carrier of active ingredients of drugs for future research.

Keywords: RATS, BLOOD, LIVER, KIDNEYS, NANO-POLYMER, PSEUDOPOLYAMINO ACIDS

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