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VITAMIN B₆ CONTENT IN COLOSTRUM AND MILK OF COWS AND BLOOD OF CALVES DURING MILKING PERIOD UNDER HYDROCHLORIDE PYRIDOXINE INFLUENCE

O. Jaremko, R. Pelenio

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Lviv National University of Veterinary Medicine and Biotechnologies named after S. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

We examined the content of vitamin B₆ in colostrum and milk of cows which was fed to calves and its concentration in blood calves from 1 to 90 days for the exogenous administration of different doses of pyridoxine hydrochloride. For the experiment, 6 groups of calves (control and five experimental), 5 animals in each group, were formed. During the experiment (from 1 to 90 days), the animals of the control and experimental groups were in identical feeding, care and maintenance conditions, and their rations were balanced in accordance with the rules of feeding young animals of the dairy period of cultivation. Calves of the control group were fed the main diet (MD), while in the experimental group, pyridoxine hydrochloride was added to the main diet (MD): Group I — 1 mg/kg body weight, II — 2, III — 3, IV — 4 and V group — 5 mg/kg of body weight. In the experiment, pyridoxine hydrochloride was used in powder, which was prescribed once a day during morning feeding. The material for the research was fresh-milk colostrum and milk of cows and blood of calves born by them. Samples were taken at 1, 5, 21, 60 and 90 days after calving of cows and, accordingly, at 1, 5, 21, 60 and 90 days of life of calves. It has been found that vitamin B₆ content in colostrum and milk of cows given to calves of the studied groups was highest at the 1^st day after calving and was 31.4 mg/kg. At day 5, its concentration decreased to 15.6 mg/kg, and at the 21^st day — 19.6 times. Up to 60 days, a slight decrease in the content of vitamin B₆ in milk was detected, and at 90 days after calving of cows its concentration was 0.6 mg/kg of milk, which was found to be lower by 52.3 times, compared to the 1^st day. The content of vitamin B₆ in the blood of calves in the dairy period of cultivation also turned out to be the highest at the 1^st day of their lives, then decreased to 21^st day and increased to the 90^th day. For additional administration to the diet of pyridoxine hydrochloride, a probable growth (P<0.05), compared with the control group, of the content of vitamin B₆ at 5^th and 21^st day at doses of 2, 3, 4 and 5 mg/kg of body weight and on 60^th and 90^th day for doses of 3, 4 and 5 mg/kg of body weight.The optimal dose of daily supplement of pyridoxine hydrochloride for correction of vitamin-supply in calves at age 1–21 days is 4 mg/kg of body weight, in calves at age 21–90 days — 3 mg/kg of body weight.

Keywords: PHYSIOLOGY, VITAMIN B₆, CALVES, COLOSTRUM, MILK, BLOOD, PYRIDOXINE HYDROCHLORIDE

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