Bìol. Tvarin. 2020; 22 (4): 18–21.
Received 09.11.2020 ▪ Accepted 29.11.2020 ▪ Published online 30.12.2020

Activity of indicators of the humoral immunity unit of calves for the action of pyridoxine hydrochloride

О. Jaremko, M. Verkholiuk, R. Peleno, V. Semanyuk

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Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv,
50 Pekarska str., Lviv, 79010, Ukraine

The article presents the data of the effect of different doses of pyridoxine hydrochloride on the activity of humoral immunity in the blood serum of calves of the dairy growing period. The lowest activity of the indicators of humoral immunity was found in the serum of calves for the first day of life in all research groups. Exogenous administration of pyridoxine hydrochloride to colostrum and milk led to changes in the studied parameters. It was found that the bactericidal activity of serum increases on the 21st day of calf life and slightly decreases on the 90th day. The addition of pyridoxine hydrochloride at different doses to the milk leads to a probable increase in bactericidal activity of the serum only at doses of 4.0 mg/kg body weight at 60 and 90 days (P<0.05) and 5.0 mg/kg body weight from 21 90 days (P<0.05). Serum lysozyme activity increases during ontogeny and under the action of exogenous pyridoxine hydrochloride. A significant difference between the lysozyme activity indices of the control and experimental groups was established in calves of II, III, IV and V groups on the 60th and 90th day (P<0.05, P<0.01). Complementary activity of serum increases during ontogenesis, and additional introduction of pyridoxine hydrochloride has led to its decrease. A significant decrease in the complementary activity of serum was detected in calves at doses of 4 mg/kg body weight on the 90th day (P<0.05) and 5 mg/kg body weight from 21st to 90th day (P<0.05, P<0.01).

Key words: calves, bactericidal activity of blood serum, lysozyme activity of blood serum, complementary activity of blood serum, pyridoxine hydrochloride

  1. Bezukh VM, Datsenko DV, Bilochenko OV. Health status and indicators of nonspecific resistance in newborn calves. Bull. Vet. Med. 2014; 13 (108): 35–37. Available at: https://nvvm.btsau.edu.ua/uk/content/stan-zdorovya-ta-pokaznyky-nespecyfichnoyi-rezystentnosti-u-novonarodzhenyh-telyat (in Ukrainian)
  2. Broda NA, Vishchur OI, Ratsky MI, Leshovska NM, Krushelnytska ZI. “Oligovit” product impact on the natural resistance of cows and their calves. Bìol. Tvarin. 2011; 13 (1–2): 397–401. Available at: http://aminbiol.com.ua/2011pdf/62.pdf (in Ukrainian)
  3. Chumachenko VE, Vysotsky AM, Serdyuk NA, Chumachenko VV. Determination of natural resistance and metabolism in farm animals. Kyiv, Urozhay. 1990; 136. (in Ukrainian)
  4. Dorofeychuk VG. Determination of lysozyme activity of blood serum by nephelometric method. Laboratory work. 1968; 1: 28–31. (in Russian)
  5. Gumenniy VM, Humen VV, Yemets OY, Ostapenko AI. Colostrum is a liquid gold! (advice for experts on livestock). Scientific and Technical Bulletin of IT NAAS. 2015; 114: 47–57. (in Ukrainian)
  6. Kalashnikov AP, Fisina VF, Shcheglova VV, Kleimenova NI. Rates and rations for feeding farm animals. A reference manual. 2003; 422. (in Russian)
  7. Kuziv MI. Linear growth, morphological and biochemical indices of blood and natural resistance of heifers of Ukrainian black spotted milk breed to one year old. Bìol. Tvarin. 2012; 14 (1–2): 475–480. Available at: http://aminbiol.com.ua/2012pdf/74.pdf (in Ukrainian)
  8. Peleno RA. Immune status of piglets for chemoprophylaxis of nematodoze-protozoan invasion and use of probiotic “Lactovet”. Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences. 2018; 20 (88): 167–172. Available at: https://nvlvet.com.ua/index.php/journal/article/view/1782
  9. Rocha BS, Gago B, Barbosa RM, Lundberg JO, Radi R, Laranjinha J. Intragastric nitration by dietary nitrite: implications for modulation of protein and lipid signaling. Free Rad. Biol. Med. 2012; 52 (3): 693–698. https://doi.org/10.1016/j.freeradbiomed.2011.11.011
  10. Sacerdote P, Mussano F, Franchi S, Panerai AE, Bussolati G, Carossa S, Bartorelli A, Bussolati B. Biological components in a standardized derivative of bovine colostrum. Dairy Sci. 2013; 96 (3): P1745–1754. https://doi.org/10.3168/jds.2012-5928
  11. Smirnova OV, Kuzmina AT. Determination of bactericidal activity of blood serum by nephelometry. Med. Exp. Immunol. 1966; 4: 8–11. (in Russian)
  12. Urtasun R., de la Rosa LC, Nieto N. Oxidative and nitrosative stress and fibrogenic response. Liver Dis. 2008; 12 (4): 769–790. https://doi.org/10.1016/j.cld.2008.07.005
  13. Vasileva TB. Nonspecific factors of humoral immunity in animals for the vaccine “Metakol”. Reports NULES. 2016; 7 (64). (in Ukrainian) https://doi.org/10.31548/dopovidi2016.07.015
  14. Vishchur OI, Gutiy BV, Gufriy DF. Immune status, methods of assessment and methods of correction in calves of early age. A monograph. Lviv, Spolom, 2015: 183 p. (in Ukrainian)
  15. Vlizlo VV, Fedoruk RS, Ratych IB. Laboratory methods of research in biology, animal husbandry and veterinary medicine. A reference book. Lviv, Spolom. 2012; 764 p. (in Ukrainian)
  16. Vlizlo VV, Kurtyak BM, Solohub LI, Yuskiv LL, Janovich VG. The biochemical bases of vitamin feeding standardization in cows. Water soluble vitamins. Bìol. Tvarin. 2007; 9 (1–2): 43–54. Available at: http://archive.inenbiol.com.ua:8080/bt/2007/1/3.pdf (in Ukrainian)
  17. Zaroza VG, Burova GA, Burov VG. Measures to receive healthy calves and prophylaxis of their diseases. Farm Anim. 2007; 9–17. (in Russian)
  18. Zmiya MM, Golovach PI. Humoral immunity state in bull fattening for correction racion on the effect of B vitamins (В1, В2, В5, В6, В10, В12). Scientific Messenger of LNU of Veterinary Medicine and Biotechnologies. Series: Veterinary Sciences. 2016; 18 (70): 115–118. (in Ukrainian) https://doi.org/10.15421/nvlvet7027

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