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Bìol. Tvarin, 2013, volume 15, issue 2, pp. 49–55

THE PECULIARITIES OF ANTIOXIDANT DEFENSE AND LIPID METABOLISM IN THE ORGANISM OF SOWS AND PIGSUNDER CHROMIUM CITRATE ACTION

R. Ya. Iskra, V. V. Vlizlo

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Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

Theаctivity of antioxidant enzymes system, the content of reducedglutathione, vitamin E and lipids in the blood of sows and pigletsif added to their diet at doses of chromium citrate 20 and 100 µg Cr(ІІІ)/kg feed were studied. Spectrophotometric and chromatographic methods of research shown that foractionschromiumcitrate, in the amount of 100mgCr(ІІІ)/kgfeedin sowson the 5-th dayafter farrowingincreasedsuperoxide dismutase, catalase, glutathioneperoxidase andglutathione reductaseactivity oferythrocytelysate, but declined content of triacylglycerols and cholesterol.Inpigs20days oldactionchromiumcitrate,in the amount of20 mgCr(ІІІ)/kg of feed, ledto an increase insuperoxide dismutaseand glutathione reductaseactivity oferythrocytelysateandcontentof vitaminE intheir bloodplasma, and in the amount of 100mgCr(ІІІ)/kg of feed increasing the contentof reducedglutathione,glutathione peroxidaseand glutathione reductaseactivity oferythrocytelysate. These results suggesta positive effectinchromiumcitrateinvestigatedquantities inantioxidant systemin the body sows andnewbornpiglets.Reduction of triacylglycerols and cholesterol in the blood of sows second experimental group suggests corrective influence of Cr(ІІІ)on lipid metabolism in the first days after birth.

Keywords: SOW, PIG, CHROMIUM CITRATE, ANTIOXIDANT SYSTEM, LIPIDS

  1. Vincent J. The Nutritional Biochemistry of Chromium (III). Department of Chemistry The University of Alabama Tuscaloosa, USA, 2007, 277 p.
  2. Iskra R. Ya., Vlizlo V. V. Biological role of chromium in animals. The Animal Biology, 2011, vol.13, no. 1–2, pp. 31–47. (in Ukrainian)
  3. Pechova A., Pavlata L. Chromium as an essential nutrient: a review. Veterinarni Medicina, 2007, vol. 52, no. 1, pp. 1–18. https://doi.org/10.17221/2010-VETMED
  4. Cefalu W. T., Hu F. B. Role of Chromium in Human Health and in Diabetes. Diabetes Care, 2004, vol. 27, no. 11, pp. 2741– https://doi.org/10.2337/diacare.27.11.2741
  5. Jain S. K. Rains J. L., Croad J. L. High glucose and ketosis (acetoacetate) increases, and chromium niacinate decreases, IL-6, IL-8, and MCP-1 secretion and oxidative stress in U937 Antioxid Redox Signal, 2007, vol. 9, pp. 1581–1590. https://doi.org/10.1089/ars.2007.1577
  6. Jain S. , Kannan K. Chromium Chloride Inhibits Oxidative Stress and TNF-a Secretion Caused by Exposure to High Glucose in Cultured U937 Monocytes. Biochemical and Biophysical Research Communications, 2001, vol. 289, pp. 687–691. https://doi.org/10.1006/bbrc.2001.6026
  7. Anderson R. , Roussel A. M., Zouari N., Mahjoub S. Potential antioxidant effects of zinc and chromium supplementation in people with type 2 diabetes mellitus. Jounal of the American College of Nutrition, 2001, vol. 20, no. 3, pp. 212–218. https://doi.org/10.1080/07315724.2001.10719034
  8. Dubinina Ye. Ye., Salnikova L. A., Yefimova L. F. Activity and superoxide dismutase isozyme spectrum of red blood cells and plasma of human blood. Laboratory work, 1983, no. 10, pp. 30–33. (in Russian)
  9. Moin V. M. A simple and specific method for determining the activity of glutathione peroxidase in erythrocyte. Laboratory work, 1986, no. 12, pp. 724–727. (in Russian)
  10. Korolyuk M. A., Ivanova L. I., Mayorova I. G., Tokarev V. Ye. The method for determining the activity of catalase. Laboratory work, 1988, no. 1, pp. 16–18. (in Russian)
  11. Laboratory methods for research in biology, veterinary medicine. A guide. E by V. V. Vlizlo Lviv, VMS, 2012, 764 p. (In Ukrainian)
  12. Hissin P. , Hilf R. A. A fluorometric method for determination of oxidized and reduced glutathione in tissues. Analytical Biochem, 1976, vol. 74, pp. 214–226. https://doi.org/10.1016/0003-2697(76)90326-2
  13. Honchar O. O., Mankovska I. M. Adapting the glutathione system of rat hearts to action acute stress under various modes of hypoxic training. Ukrainian Biochemical Journal, 2007, vol. 79, no. 3, pp. 79–85 (in Ukrainian).
  14. Tezuka M. Chromium (III) decreases carbon tetrachloride originated trichloromethyl radical in mice. Inorg. Biochem., 1991, vol. 44, pp. 261–265. https://doi.org/10.1016/0162-0134(91)84031-4
  15. Preuss H. G. The insulin system: influence of J. Am. Coll. Nutr., 1998, vol. 17, pp. 101–102. https://doi.org/10.1080/07315724.1998.10718732
  16. Sahin K., Onderci M., Sahin N., Aydin S. Effect of dietary chromium picolinate and ascorbic acid supplementation on egg production, egg quality and some serum metabolites of laying hens reared under a low ambient temperature (6 °C). Arch. Tierernahr, 2002, vol. 56, no. 1, pp. 41–49. https://doi.org/10.1080/00039420214174
  17. Peter J. Espenshade SREBPs: sterolregulated transcription factors. Journal of Cell Science, 2006, vol. 119, pp. 973–976. https://doi.org/10.1242/jcs02866

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