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Bìol. Tvarin, 2013, volume 15, issue 2, pp. 90–96
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RESISTANCE OF THE RABBIT DAMS’ ORGANISM AT DRINKING SUPPLEMUTED BY CHLORELLA SUSPENSION, SODIUM SULFATE, CHROMIUM CHLORIDE AND CITRATE

Ya. V. Lesyk

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Institute of Animal Biology NAAS,
38 Stus str., Lviv 79034, Ukraine

The aim at resealed was to study the influence of Chlorella suspension, sodium sulfate, chromium chloride and citrate on the physiological blood indices and nonspecific resistance of female rabbits during the period from copulation to the lactation peak. The number of erythrocytes, hemoglobin concentration, the aminotransferases activity, phagocytic activity of neutrophils, lysozyme and bactericidal activity of rabbitdams’ blood serum at introduction of Chlorella suspension, sodium sulfate, citrate and chromium chloride into the ration were determined. Researches were conducted on silver breed rabbits’ females, 110–114 days age, weighing 3.7–3.9 kg divided into five groups (one control and four experimental) 12 females each, selected on the basis of analogues principle. Control group rabbits were fed enough balanced granulated mixed fodder with free access to water. The 1st experimental group, except for mixed fodder, starting from the first research day received 90–110 ml of Chlorella suspension with water. The second experimental group’s animals were fed mixed fodder similarly to I experimental group’s schemes and drink, except for Chlorella, sodium sulfate in an amount of 40.0 mg/kg body weight or 0.15–0.17 g S per day. Females of the third experimental group received mixed fodder and water according to II group’s scheme with extra drinking Chromium as CrCl3 х 6Н2О, in the amount of 28–32 mg Cr per day at the rate of 7.6 mg/kg of body weight. Animals of the fourth experimental group received mixed fodder and water according to the II group’s schemes with additional introduction into the water of Chromium citrate obtained by using nanotechnology at the rate of 2.2 mg Cr/kg body weight or 8–12 mg Cr per day. Researched established that in the blood of II, III and IV research groups’ rabbits that additionally consumed sodium sulphate, chloride and chromium citrate, the number of erythrocytes, hemoglobin and total protein concentration, and activity of alanine aminotransferase were credibly higher on the 67 day of the study compared to the control group. Introduction to the diet of II, III and IV groups’ rabbits of mineral supplementation resulted in high immunological reactivity of organism with the increase of phagocytic activity of neutrophils, lysozyme and bactericidal activity level in the blood compared with animals of the control group.

Кeywords: RABBITS, CHROMIUM, PHYSIOLOGICAL BLOOD PARAMETERS, NONSPECIFIC RESISTANCE

  1. Trocino A., Xiccato G. Animal welfare in reared rabbits: a review with emphasis on housing systems. World Rabbit Sci., 2006, vol. 14, pp. 77–93.
  2. Shylov Yu. Y., Shylov S. Yu., Lanyn D. R. Adrenergic mechanisms regulationof immune response and functions of non-specific effector cells under stress. Medical immunology, 2005, vol. 7, no. 2–3, pp. 127–128. (in Ukrainian)
  3. Carabaño R., Badiola I., Chamorro S., García J., García-Ruiz A. I., García-Rebollar P., Gomez-Conde M. S., Gutiérrez I., Nicodemus N., Villamide M. J., de Blas J. C. New trends in rabbit feeding: Influence of nutrition on intestinal health. Spanish J. Agri. Res., 2008, vol. 6, pp. 15–25. https://doi.org/10.5424/sjar/200806S1-5346
  4. Maertens L., Štruklec M. Technical note: Preliminary results with a tannin extract on the performance and mortality of growing rabbits in an enteropathy infected environment. World Rabbit Sci., 2006, vol. 14, pp. 189–192.
  5. Mann D. L. Stress activated cytokines and the heart. Ann. Rev. Physiol., 2003, vol. 65, pp. 81–101. https://doi.org/10.1146/annurev.physiol.65.092101.142249
  6. Sein O. B., Umerenkov I. A., Trubnikov D. V. Biochemical and immunological parameters of rabbits at acceleration breeding method. Achievements of modern science, 2005, no. 10, pp. 53–53. (in Russian)
  7. Wieloch A., Wieczorek D., Staniek H., Szymusiak H., Krejpcio Z., Zieliński R. Tricentric complexes of chromium (III) with glycine and serine ligands. Current Trends in Commodity Science., 2007, pp. 1080–1086.
  8. Chumachenko V. Yu., Chumachenko V. V., Pavlenko O. A. Resistance of animals and factors affecting its status. The study of the immune system. Factors affecting the resistance of animals. Veterinary Medicine of Ukraine, 2004, no. 4, pp. 26–29. (in Ukrainian)
  9. Bashchenko M. I., Honchar O. F., Shevchenko Ye. A. The Rabbits Farming. Cherkasy, Cherkasy institute of APV, 2011. 302 p. (in Ukrainian)
  10. Wang M. Q., Li H., He Y. D., Wang C., Tao W. J., Du Y. J. Efficacy of dietary chromium (III) supplementation on tissue chromium deposition in finishing pigs. Biol Trace Elem Res., 2012, vol. 148, no. 3, pp. 316–321. https://doi.org/10.1007/s12011-012-9369-x
  11. Rao S. V., Raju M. V., Panda A. K., Poonam N. S., Murthy O. K., Sunder G. S. Effect of dietary supplementation of organic chromium on performance, carcass traits, oxidative parameters, and immune responses in commercial broiler chickens. Biol Trace Elem Res., 2012, vol. 147, no. 1–3, pp. 135–141.
  1. Staniek H., Krejpcio Z., Iwanik K., Szymusiak H., Wieczorek D. Evaluation of the Acute Oral Toxicity Class of Trinuclear Chromium (III) Glycinate Complex in Rat. Biol Trace Elem Res., 2011, vol. 143, no. 3, pp. 1564–1575. https://doi.org/10.1007/s12011-011-8959-3
  2. Kravtsiv R. Y., Maslyanko R. P., Kravtsiv Yu. R. Current state of the phagocytosis theory. Scientific Messenger of Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky, 2005, vol. 7, no. 2, part 1, pp. 71–77. (in Ukrainian)
  3. Kalashnikov A. P. Norms and feeding rations of agricultural animals. A handbook. Moscow, Ahropromizdat, 2003, 345 p. (in Russian
  4. Kosinov M. V., Kaplunenko V. H. Method for metal carboxylates obtaining “Nanotechnology of obtaining metals’ carboxylates”, Patent of Ukraine no. 38391, 2009. (in Ukrainian)
  5. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of research in biology, and veterinary medicine. A guide. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
  6. Moscati L., Dal Bosco A., Battistacci L., Cardinali R., Mugnai C., Castellini C. Native immunity and oxidative traits of growing rabbits. World Rabbit Sci., 2008, vol. 16, pp. 213–220.

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