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OXIDATIVE STRESS BIOMARKERS AND ERYTHROCYTE HEMOLYSIS IN TRAINED Ukrainian Warmblood HORSES UNDER THE INFLUENCE OF EXERCISE

Anastasiia Andriichuk1, Halyna Tkachenko2,Natalia Kurhaluk2, Iryna Tkachova1

1Institute of Animal Breeding of National Academy of Agricultural Sciences, Kharkiv, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.

2Department of Animal Physiology, Department of Zoology, Institute of Biology and Environment Protection, Pomeranian University, Słupsk, Poland, This email address is being protected from spambots. You need JavaScript enabled to view it.This email address is being protected from spambots. You need JavaScript enabled to view it.; This email address is being protected from spambots. You need JavaScript enabled to view it.

The purpose of the present study was to investigate the effect of regular exercises on oxidative stress biomarkers, antioxidant enzymes activity, as well as resistance of erythrocytes to hemolytic agent in well-trained Ukrainian warmblood horses. Twenty threeUkrainian warmblood horses (9 females and 14 males), involved in jumping, eventing and dressage were used. Oxidative stressbiomarkers (2-thiobarbituric acid reactive substrates level (TBARS), the carbonyl derivatives content of protein oxidative modification),antioxidantdefenses (superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx), level of ceruloplasmin (CP), total antioxidant capacity), and osmotic resistance of erythrocytes were assessed. Our results suggest that exercises cause different consequences in oxidative stress biomarkers in the blood, plasma, and erythrocytes of horses. Exercise lead to decrease TBARS level in erythrocytes while in blood and plasma does not. This difference in TBARS level before and after exercise, most likely is a consequence of differing levels of oxidative stress occurring in the tissues and erythrocytes. Decrease of carbonyl derivatives in erythrocytes’ suspension is result of exercise-induced adaptation.A correlation between the oxidative stress biomarkers and antioxidant defenses in the horses after exercise was observed, which may indicate a protective response of GR, GPx and CP activities against exercise-induced oxidative stress. Statistically significant differences in the percentage of haemolysed erythrocytes between before and after exercise also were observed.

Keywords: HORSES, Ukrainian warmblood BREED, TRAINING, OXIDATIVE STRESS, ANTIOXIDANT DEFENSES, ERYTHROCYTE RESISTANCE

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