The Animal Biology

sperm Respiration intensity and survival ofspermatozoawith taurine ADDITION in dilutedbull ejaculates

R. D. Ostapiv^1,2, V. V. Manko¹, I. M. Yaremchuk³, D. D. Ostapiv³

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Semen respiration intensity and spermatozoa survival by using diluents with different taurine concentration inbull ejaculates was studied. Intensity of oxygen consumption by semen was determined polarographycally with Clark electrode usage (at temperature 38.5°С), spermatozoa survival — by storage with dilution (at temperature 0–4° С) till linear forward motion is stopped (h). To diluted semen, with phosphate-saline buffer and synthetic mediums (lactose-yolk-glycerin diluent and «Andromed») taurine in concentrations 0.175; 0.35; 0.525 and 0.7 mmol/l was added. In our studies we determined correlation between semen respiration rate and spermatozoa survival, with using diluents and at action of taurine in different concentrations. With taurine concentration increasing, sperm respiration intensity decreased. The biggest decrease of indexes was after diluting semen with phosphate-saline buffer and the smallest — after mixing with Andromed. By diluting semen with phosphate-saline buffer, survival increases with taurine concentration. When bull ejaculates were diluted by lactose-yolk-glycerin diluents spermatozoa survival dependence had a maximal index with addition 0.35 mmol/l, though changes haven’t reached first degree of probability.In case of sperm dilution by Andromed, spermatozoa survival decreased with taurine concentration increase, and while adding 0.7 mmol/l, was lower on 18.2 %comparing to control. Possible reason for positive taurine impact on spermatozoa survival is ability to maintain transmembrane mitochondrial potential and influence on substrate utilization, its antioxidant properties, in case of negative effect — ability to cotransport with Na⁺, that decreases spermatozoa motility.

Keywords: RESPIRATION INTENSITY, TAURINE, SPERMATOZOA SURVIVAL, SEMEN, BULL

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