ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISM IN THE CALPAIN/CALPASTATIN SYSTEM GENES WITH QUANTITATIVE TRAITS OF ABERDEEN-ANGUS

Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 100–110

http://doi.org/10.15407/animbiol19.01.100

ASSOCIATION OF SINGLE NUCLEOTIDE POLYMORPHISM IN THE CALPAIN/CALPASTATIN SYSTEM GENES WITH QUANTITATIVE TRAITS OF ABERDEEN-ANGUS

S. Ruban¹, O. Fedota², N. Lysenko², A. Kolіsnik³, І. Goraichuk⁴, L. Ponko⁵

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¹Institute of Animal Breeding and Genetics named after M. V. Zubets NAAS,
1 Pogrebnyaka str., Chubynske, Kyiv region, 08321, Ukraine

²V.N.Karazin Kharkiv National University,
4 Svobody sq., Kharkiv 61022, Ukraine

³PE «Agrofirma Svitanok»,
11 Marksa str., Novoselivka, Kharkiv region, 63209, Ukraine

⁴National Scientific Center “Institute of Experimental and Clinical Veterinary Medicine”,
83 Pushkinska str., Kharkiv 61023, Ukraine

⁵State agrarian and engineering university in Podilya,
13 Shevchenko str., Kamyanets-Podilsky, Khmelnytsky region, 32300, Ukraine

Pleiotropic effect of calpain/calpastatin system genes influences both animal’s quantitative traits and qualitative characteristics of muscle tissue. The study is aimed to evaluate the association of single nucleotide polymorphisms CAPN316 and CAST282 in calpain and calpastatin genes with quantitative traits — the body weight dynamics.

The body weight dynamics from birth until 5 years of age was evaluated in Aberdeen-Angus cows (n=73) in Kharkiv region. Molecular genetic analysis was performed by PCR-RFLP. Distributions was analyzed with χ² criterion, ANOVA was used for multiple comparisons. The influence of individual factor was evaluated by Snedecor (h²). Differences between Aberdeen Angus groups was studied by Nei’s standard genetic distances.

Allele frequencies of SNP CAPN316 are C=0.404 and G=0.586, of SNP CAST282 are C=0.788 and G=0.212. The genotypic distributions were in agreement with the Hardy-Weinberg equilibrium. There were significant impact of CAPN316 CC genotype on body weight at the age of three (F=3.57; h²=46%) and four years (F=3.70; h²=47%) and CAST282 CG genotype on body weight at the age of 15 months (F=3.29; h²=43%). Effect of calpain and calpastatin genes C-alleles on weight gain increases after the age of two years, when the expression of genes regulating growth and development processes at an early age is reduced. Animal selection at the age of two years aimed to the meat quality improvement is unlikely to lead to significant body weight decrease.

Keywords: ABERDEEN-ANGUS, CALPAIN, CALPASTATIN, CAPN316, CASTS282, BODY WEIGHT

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