DETERMINATION OF ANTIBACTERIAL PROPERTIESOF TRIAZINES IN REGARDS TO SALMONELLA TYPHIMURIUM

Bìol. Tvarin, 2017, Volume 19, Issue 1, pp. 16–23

http://doi.org/10.15407/animbiol19.01.016

DETERMINATION OF ANTIBACTERIAL PROPERTIESOF TRIAZINES IN REGARDS TO SALMONELLA TYPHIMURIUM

M. M. Babkina^1,4, O. V. Vasylchenko², O. M. Deriabin³, A. A. Tarasov⁴,A. M. Golovko³, L. G. Palchykovska²

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¹Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

²Institute of Molecular Biology and Genetics National Academy of Sciences of Ukraine,
150 Akademika Zabolotnoho str., Kyiv 03680, Ukraine

³State Scientific Control Institute of Biotechnology and States of Microorganisms,
30 Donetska str., Kyiv 03151, Ukraine

⁴Institute of Veterinary Medicine NAAS,
30 Donetska str., Kyiv 03151, Ukraine

The results of the study of antibacterial activity and determining minimum inhibitory concentrations triazines classes by microdilution method against a Salmonella typhimurium are described in the article. The antimicrobial activity of the triazines class substances was investigated by disk diffusion method. An antibacterial activity of triazines to museum strain Salmonella typhimurium was established. The presence of the susceptibility of Salmonella typhimurium to the tested compounds has been revealed.

The monocyclic and threecyclic triazines compounds classes were used. We chose eleven the most perspective substances with some difference due to position of radical groups and its composition recording the results of the research.

As result of investigations the Minimal Inhibitory Concentration and growth inhibition zone of monocyclic and threecyclic triazines class substances were identified. The Minimal Inhibitory Concentration was detected at range from 0.41±0.005 mg/сm³ to 0.0041±0.00007 mg/cm³and growth zone diameter from 9.3±0.49 mm to 24±0.36 mm.

The most active compounds tested of substances regards to Salmonella typhimurium were ODI-8 (6-[(4-aminophenyl)amino]-1,2,4-triazine-3,5(2H,4H)-dione), ODI-13 (6-[(4-isopropoxyphenyl)amino]-1,2,4-triazine-3,5(2H,4H)-dione), ODI-45 (7-methyl-3-oxo-2,3-dihydro-1H-[1,2,4]triazino[5,6-b][1,4]benzothiazine-9-carboxylic acid) and ODI-60 (7-methyl-3-oxo-N-pyrimidin-2-yl-2,3-dihydro-1H-[1,2,4]triazino[5,6-b][1,4]benzothiazine-9-carboxamide) with a minimum inhibitory concentration 0.0041 mg/cm³ and the diameter of growth inhibition zone from 22.6±0.61 mm to 24±0.36 mm. ODI-11 has activity with a minimum inhibitory concentration 0.041 mg/cm³ and the diameter of growth inhibition zone 21.1±0.47 mm.

The compounds ODI-5, ODI-10, ODI-14, ODI-15, ODI-50 and ODI-58 which differ by nature and position of the radicals, demonstrated low level of antibacterial activity with a minimal concentration 0.41 mg/cm³. The diameter of the zones of growth inhibition for these compounds was from 9.3±0.49 mm to 11.3±0.49 mm.

The compounds ODI-1, ODI-2, ODI-3, ODI-4, ODI-6, ODI-7, ODI-9, ODI-12, ODI-46, ODI-47, ODI-48, ODI-49, ODI-51, ODI-52, ODI-53, ODI-54, ODI-55, ODI-56, ODI-57, ODI-59 did not demonstrate any activity against Salmonella typhimurium.

Keywords: MICROORGANISM, SALMONELLA TYPHIMURIUM, MINIMAL INHIBITORY CONCENTRATION, ANTIBACTERIAL PROPERTIES, TRIAZINES

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