Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 115–120
INDICIES OF FATTY ACIDS SPECTRUM OF LIPIDS IN THE BLOOD SERUM OF RATS UNDER CONDITIONS OF ARTIFICIAL HYPOBIOSIS
A. A. Umanska1, D. A. Melnychuk1, S. D. Melnychuk2, L. H. Kalachniuk1, V. S. Morozova1
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1National University of Life and Environmental Sciences of Ukraine,
15 Heroiv Oborony str., Kyiv 03041, Ukraine
2China-Ukraine Research Life Science Institute,
135 West gen ta road, office 204, Zhuji city, Zhejiang province, PRC
Methodical approaches to anesthesia, blood conservation, etc. with entering an organism into a state of artificial hibernation, a prerequisite of which is hypoxia, hypercapnia, and hypothermia, were and are promising and relevant for medical research. Under conditions of a reduced level of life of the organism in a low-temperature environment, lipid substances play one of the key roles in the functioning of biological membranes and lipid metabolism regulation. Therefore, to study the adaptation functional characteristics of the organism, it was identified content of triacylglycerols, cholesterol, high-carboxylic acids and high (HDL), low (LDL) and very low (VLDL) density lipoproteins in the blood serum of rats under conditions of normal, artificial hypo-biotic state and in 24 hours after leaving hypo-biotic state.
Under the effect of such factors, as hypercapnia, hypoxia, and hypothermia, rats were entered in a state of artificial hibernation. We determined the content of the above lipid substances by conventional methods in rat serum of three groups: control (1st) and two experimental (2nd — a state of artificial hibernation and 3rd — in 24 hours after leaving hypobiosis).
It was found that the fatty acid spectrum of lipids in the blood serum qualitatively remained without changes during the hypo-biotic state, while the significant redistribution was quantitatively observed, namely increasing myristic, palmitic and stearic acids and reducing linoleic, linolenic and docosahexaenoic acids.
Cholesterol-, LDL- and HDL-content remained without considerable changes in the blood serum of rats of all groups, while it was observed significant increase of a number of triacylglycerols and VLDL (by ~30 %) in animals at the state of artificial hypobiosis and in 24 hours after leaving it.
Keywords: LIPIDS, LIPOPROTEINS, HYPOBIOSIS, HYPERCAPNEA, HYPOXIA, BLOOD SERUM, RATS
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