Bìol. Tvarin, 2017, Volume 19, Issue 2, pp. 16–22
EXPRESSION OF IMUNORECEPTOR PROTEINS IN PLASMOLEMMA OF ENTEROCYTES IN FORMATION OF COLOSTRIC IMMUNITY IN NEWBORN CALVES
S. I. Golopura, M. O. Marinyuk, M. I. Tsvilikhovsky
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National university of life and environmental sciences of Ukraine,
16 Polkovnika Potekhina str., Kyiv 03041, Ukraine
Indexes of content of proteins with molecular masses of 37, 40 and 43 kDa in plasmolemma of small intestine enterocytes in newborn calves in dynamics from the birth till the age of 24 hours were investigated. The research was conducted on two groups (control and research) of the newborn calves of Ukrainian black-and-white breed; the samples of jejunum enterocytes were taken and the content of proteins from membrane fraction of lysates was isolated. The relatively high content of expression of proteins with М? of 37, 40 and 43?kD ? in membrane fraction of lysates of enterocytes from jejunum of just born calves of control group with further significant decreasing of total content of these proteins at the age of 6 hours and with their following increasing at the age of 24 hours comparatively with the previous one was shown.
М of 37, 40 and 43 kD а in membrane fraction of lysates of enterocytes from jejunum of just born calves of control group with further significant decreasing of total content of these proteins at the age of 6 hours and with their following increasing at the age of 24 hours comparatively with the previous one was shown.
The application of macrocapsules from phospholipid bilayer on the basis of soy-bean lecithin for newborn calves activates the Ig transport in small intestine during the period of colostral immunity formation that assists the increasing of its level. The total content of proteins with MM of 37, 40 and 43 kDa in plasmolemma of jejunum enterocytes in calves of the research group at the age of 6 hours significantly decreased with its further increasing at the age of 24 hours compared to these indexes in calves from control group. The dynamics with comparative analysis between fractions of expression of proteins with MM of 37, 40, and 43 kDa in plasmolemma of jejunum enterocytes in the newborn calves from control and research group is shown. The application of macrocapsular preparation comparing to the control group indicates more intensive level of recycling of receptor proteins through enterocytes from BM to AM with further reception of colostrum Ig and their transcellular transportation.
Keywords: NEWBORN CALVES, SMALL INTESTINE, ENTEROCYTE, PLASMOLEMMA, PROTEINS, PHOSPHOLIPID, MACROCAPSULES, COLOSTRIC IMMUNITY
1. Abrahamson D. R., Rodewald R. Evidence for the sorting of endocytic vesicle contents during the receptor-mediated transport of IgG across the newborn rat intestine.
J. Cell Biol., 1981, vol. 91, no. 1, pp. 270–280.
https://doi.org/10.1083/jcb.91.1.2702. Astier A., Salle H., Salle C., Bieber T., Esposito-Farese M. E., Freund M., Cazenave J. P., Fridman W. H., Teillaud J. L., Hanau D. Human epidermal Langerhans cells secrete a soluble receptor for IgG (Fc gamma RII/CD32) that inhibits the binding of immune complexes to Fc gamma R+ cells. J. Immunol., 1994, vol. 152, no. 1, pp. 201–212.
3. Goebl N. A. Goebl C. M., Babbey A., Datta-Mannan D. R., Witcher V. J., Wroblewski K. W., Dunn N. A. Neonatal Fc Receptor Mediates Internalization of Fc in Transfected Human Endothelial Cells.
Mol. Biol. Cell., 2008, vol. 19, no. 12, pp. 490–505.
https://doi.org/10.1091/mbc.E07-02-01014. Hovalo V. I. Immunology of reproduction. Moscow, Medicine, 1987, 304 p. (in Russian)
5. Ivo Miler. Immunity of the human fetus and newborn. Prague, 1983, 232 p. (in Russian)
6. Jones E. A., Waldman T. A. The mechanism of intestinal uptake and transcellular transport of IgG in the neonatal rat.
J. Clin. Invest., 1972, vol. 51, p. 2916.
https://doi.org/10.1172/JCI1071167. Kaplun A. P., Le Bang Shon, Krasnopolsky U. M., Shvets V. I. Liposomes and other nanoparticles as a means of drug delivery. Problems of Medical Chemistry, 1999, vol. 4, no. 1, pp. 3–12. (in Russian)
8. Karmoliev R. X. Immunosuppressive processes in colostral immunity. Veterinary science, 1993, no. 6, pp. 27–29. (in Russian)
9. Marinyuk M. O., Golopura S. I., Yakimchuk O. M., Nemova T. V., Tsvilikhovsky M. I. Kolostral level of immunity and development of digestive disorders in newborn calves. Veterinary Medicine of Ukraine, 2014, no. 5, pp. 21–23. (in Ukrainian)
10. Masyuk D. M., Nedzvetsky V. S., Tsvilikhovsky M. I., Nerush P. O. The changes of expression and fc-g-receptor.s polypeptide composition of small intestines enterocyte of bos rimigenius taurus l. Fetus. Physiological Journal, 2008, vol. 54, no. 1, pp. 27–34. (in Ukrainian)
11. Masyuk D. M., Nedzvetsky V. S., Tsvilikhovsky M. I. Prenatal modulation of expression of receptors to immunoglobulin G intestinal cell of cattle. Scientific and technical bulletin SIC biosafety and environmental control resources AIC, 2012, vol. 1, no. 1, pp. 90–94. (in Ukrainian)
12. Mayr A. Muttertier — Schutzimpfung. Berl. Munch. Tierarztl. Wschr., 1982, vol. 95, no. 18, pp. 341–350.
13. Meerovitch I. G., Oborotova N. A. The use of liposomes in photochemotherapy. Russian Journal of biotherapeutic, 2004, vol. 2, no. 4, pp. 3–8. (in Russian)
15. Schägger H., von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.
Analytical Biochemistry, 1987, vol. 166, no. 2, pp. 368–379.
https://doi.org/10.1016/0003-2697(87)90587-216. Tsvilikhovsky M. I. Proteins plasma membrane epithelium of the small intestine of cattle. Dr. biological sci. diss. Kyiv, 1998, 38 p. (in Ukrainian)
17. Usatuk P. V. Biochemical characterization of plasma membrane and features regulation epithelium of the small intestine of cattle in ontogenesis and in dysfunction. Dr biological sci. diss. Kyiv, 1994, 43 p. (in Ukrainian)
18. Vladimirov Y. A. Calcium pumps living cells. Soros Educational Journal, 1998, no. 3, pp. 20–27. (in Russian)
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