Bìol. Tvarin, 2017, volume 19, issue 3, pp. 122–127

EFFECTS OF THE COMPLEX OF MAGNESIUM AND CHROMIUM CITRATES ON THE CARBOHYDRATE METABOLISM IN THE BLOOD OF RATS WITH EXPERIMENTAL DIABETES MELLITUS

O. A. Shatynska, R. Ya. Iskra, O. Z. Svarchevska

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Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

The article presents the results of investigation of the influence of the complex of magnesium (Mg2+) and chromium (Cr3+) citrates on the activity of carbohydrate metabolism key enzymes, and also on the concentration of insulin and C-peptide in the blood of rats with experimental diabetes. The complex of magnesium and chromium citrates in doses 250 mg Mg/kg bw plus 25 mkg Cr/kg bw and 250 mg Mg/kg bw plus 10 mkg Cr/kg bw was added to the drinking water for all animals during 30 days. Experimental diabetes in animals was induced by a single intraperitoneal administration of a solution of alloxane monohydrate (“Synbios”) at a dose of 150 mg/kg body weight. The glucose-6-phosphate dehydrogenase (G-6-PDH) and lactate dehydrogenase (LDH) activities in erythrocyte lizates, glucose, insulin and C-peptide concentrations in blood plasma were investigated.

It was found that experimental diabetes indused the decreased of glucose-6-phosphate dehydrogenase activity, insulin and C-peptide concentrations, and the increased lactate dehydrogenase activity and glucose concentration. The complex of magnesium and chromium citrates, which was added with water to the animals’ diet during four weeks, led to a positive normalizing effect. It was shown by a credible increase of insulin and C-peptide concentrations and followed by a decrease of glucose concentration in the blood plasma.

Thus received results demonstrate that the addition of the investigated complex compound may become a basis for a development a new methods, which will help to correct the emergence and development of complications for diabetes mellitus in future.

Keywords: DIABETES MELLITUS, CARBOHYDRATE METABOLISM, INSULIN, MAGNESIUM CITRATE, CHROMIUM CITRATE

  1. Barbagallo M., Dominguez L. J., Brucato V., Antonio Galioto A., Pineo A., Ferlisi A., Tranchina E., Belvedere M., Putignano E., Costanza G. Magnesium metabolism in insulin resistance, metabolic syndrome, and type 2 diabetes mellitus. New perspectives in magnesium research, Springer London, 2007, pp. 213–223.
  2. Cefalu W. T., Hu F. B. Role of chromium in human health and in diabetes. Diabetes Care, 2004, vol. 27, no. 11, pp. 2741–2751. https://doi.org/10.2337/diacare.27.11.2741
  3. Chetan P. Hans, Dharam P. Chaudhary, Devi D. Bansal. Effect of magnesium supplementation on oxidative stress in alloxanic diabetic rats. Magnesium Research, 2003, vol. 16, no. 1, pp. 13–19.
  4. Fediv O. I., Marchuk Yu. F., Voloshyna L .A. Peculiarities of an affection of the hepatobiliary systems in patients with diabetes mellitus. Bukovinian Medical Herald, 2008, vol. 12, no. 4, pp. 126–131. (in Ukrainian)
  5. Galenova T., Raksha N., Savchuk O., Ostapchenko L. Functioning of some key enzymes of carbohydrate metabolism in rats under experimental type 2 diabetes. Experimental and Clinical Physiology and Biochemistry, 2011, vol. 2, no. 54, pp. 13–21. (in Ukrainian)
  6. Gulich M. P., Kharchenko O. O., Yemchenko N. L., Yermolenko V. P., Moiseienko I. Ye. Magnesium citrate, obtained on the basis of aquananotechnology: chemical and biological characteristics. Environment and health, 2014, vol. 71, no. 4, pp. 14–18.
  7. Iskra R. Ya, Vlizlo V. V. Peculiarities of antioxidant defense system in erythroid cells and tissues of pigs under action of chromium chloride. The Ukrainian Biochemical Journal, 2012, vol. 85, no. 3, pp. 96–102. (in Ukrainian) https://doi.org/10.15407/ubj85.03.096
  8. Iskra R. Ya. Some indices of antioxidant protection and carbohydrates metabolism in rats’ tissues at chromium nanocytrate action. Studia Biologica, 2011, vol. 5, no. 3, pp. 49–58.
  9. Maritim A. C., Sanders A., Watkins 3. J. Diabetes, oxidative stress, and antioxidants: a review. Journal of biochemical and molecular toxicology, 2003, vol. 17, no. 1, pp. 24–38. https://doi.org/10.1002/jbt.10058
  10. Menshikov V. V. Laboratory Research Methods in Clinic. Medicine, 1987, pp. 174–177. (in Russian)
  11. O’Connell B. S. Select vitamins and minerals in the management of diabetes. Diabetes Spectrum, 2001, vol. 14, no. 3, pp. 133–148. https://doi.org/10.2337/diaspect.14.3.133
  12. Pankiv V. I. Diabetes Mellitus: Diagnostic Criteria, Etiology and Pathogenesis. International journal of endocrinology, 2013, vol. 8, no. 56, pp. 53–68. (in Ukrainian)
  13. Prystupok O. Metabolic therapy for patients with diabetes mellitus. International journal of endocrinology, 2010, vol. 6, pp. 6–11. (in Ukrainian)
  14. Smolyar V. I., Petrashenko H. I. Alimentary hypo- and hypermicroelementosis. Problems of nutrition, 2005, vol. 4, pp. 40–42. (in Ukrainian)
  15. Suslyk A. I. Peculiarities of trace element content in the blood of patients with type 2 diabetes mellitus and obesity. Experimental and Clinical Physiology and Biochemistry, 2012, vol. 59, no. 3, pp. 54–59. (in Ukrainian)
  16. Ta S. Diagnosis and classification of diabetes mellitus. Diabetes care, 2014, vol. 37, pp. 81–90. https://doi.org/10.2337/dc14-S081
  17. Vincent J. B. The biochemistry of chromium. The Journal of nutrition, 2000, vol. 130, no. 4, pp. 715–718. https://doi.org/10.1093/jn/130.4.715
  18. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of research in biology, stockbreeding and veterinary medicine. A handbook. Ed. by. V. V. Vlizlo. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
  19. Volpe S. L. Magnesium in disease prevention and overall health. Advances in Nutrition: An International Review Journal, 2013, vol. 4, no. 3, pp. 378–383. https://doi.org/10.3945/an.112.003483

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