The Animal Biology

Bìol. Tvarin, 2017, volume 19, issue 4, pp. 83–87
http://doi.org/10.15407/animbiol19.04.083

ANTIBACTERIAL ACTIVITY OF COMPLEX OF ENROFLOXACIN WITH NANOPOLYMER GLULA-DPG-PEG600

O. Chekh¹, I. A. Dron², S. I. Vynnytska², V. V. Oleksa², I. E. Atamaniuk³, V. V. Vlizlo¹

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¹Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

²National University “Lviv Polytechnic”,
12 Bandery str., Lviv 79013, Ukraine

³State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

Keywords: NANOPOLYMERS, PSEUDO-POLYAMINO ACIDS, ANTIBIOTICS, ENROFLOXACIN, MICROBIAL CELLS, ANTIBACTERIAL ACTIVITY

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HISTOSTRUCTURE OF INTERNAL ORGANS IN FEMALE RATS OF FIRST GENERATION FOR DIFFERENT DOSES OF GERMANIUM CITRATE

M. I. Khrabko¹, R. S. Fedoruk¹, Yu. V. Martyn¹, U. I. Tesarivska², M. I. Shumska²

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¹ Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

²State Scientific-Research Control Institute
of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

The structural differences of the spleen, thymus, liver and lymph nodes of female rats of first generation (F₁) under the influence of various doses of nano-germanium citrate (NGeCit) have been studied. The experimental part of the work was performed on the F₁ female rats which were watered various quantities of germanium citrate, obtained by the method of nanotechnology. Female research groups, in contrast to the control (I), were given 10 (II), 20 (III) and 200 (IV) mg Ge/kg of body weight from the period of drinking water consumption, during growth, development and pregnancy. Tissue samples of F₁ female in control and experimental groups were taken on the 21^st day of pregnancy.

Morphostructural changes of internal organs were determined using standard methods of morphological and histological studies which revealed some differences in action of different doses of NGeCit in F₁ females of research groups compared with the control. Histostructural changes of hepatocyte size with their increase, deformation of veins and stratification of stroma around them in F₁ animals at the action of 20 μg Ge/kg of body mass were established. The action of 200 μg Ge/kg of body weight in F₁ females caused a violation of the organ hemodynamics and dilatation of the walls of its vessels. The use of 10 and 20 μg Ge/kg body weight in F₁ females led to an improvement in the morphofunctional state and histostructural characteristics of the thymus, whereas 200 μg Ge/kg body weight caused moderate stromal edema and narrowing of the cortical zone of this organ.

The established differences in the morphostructure of the internal organs of F₁ female rats for prolonged action of NGeCit may be determined by the dose of its application. More pronounced morphological and histological changes are noted in the organs of F₁ females for the action of 200 μg Ge/kg of body weight.

Keywords: RATS, GERMANIUM CITRATE, HISTOSTRUCTURE, ORGANS

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BLOOD SERUM BIOCHEMICAL INDICES OF DIFFERENT CATEGORIES OF PIGS BY QUALITATIVE COMPOSITION OF MUSCLE TISSU

I.Khalak¹, O. S. Grabovska², I. V. Luchka², G. G. Denys²

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¹State institution “Institute of grain crops NAAS”,
14 Volodymyra Vernadskoho str., Dnipro 49027, Ukraine

²Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

The article presents the results of studies of biochemical parameters of blood serum in young pigs taking into account their distribution into categories according to physico-chemical properties and chemical composition of muscle tissue.

Stages of scientific research work were: selection of young pigs of large white breed at 2.5–3 months of age, control fattening of young pigs to reach live weight of 100–110 kg, control slaughter and selection of longest muscle back, research of physical and chemical properties and chemical composition of the m. Longissimus dorsi. Blood samples were taken from the animals of the experimental group at the age of 5 months, the biochemical parameters of the blood serum were examined taking into account the content of total protein and cholesterol, albumin and globulin concentrations, activity of alkaline phosphatase, Aspartate aminotransferase (ASAT) and alanine aminotransferase (ALT).

It has been established that young pigs of the category “low quality” of meat in terms of moisture retention, tenderness and intramuscular fat content are characterized by a higher total protein content (by 0.67–6.73 g/l), activity, compared to peers of the “high quality” category Asat (Ast) (by 0.06–0.31 mmol/h/l) and alkaline phosphates (by 4.89–47.56 units/l).

The quantity of samples of high quality with respect to water retention capacity is 8.0 %, the intensity of coloring is 20.0 %, the content of intramuscular fat is 16.0 %, and the tenderness is 8.0 %. Reliable correlation coefficients were established for the following pairs of characteristics: “softness × cholesterol content” (r=0.420±0.1892, tr=2.22) and “color intensity, extinction × 1000 × activity of alkaline phosphates (r = –0.483±0.1826, tr=2.64).

Keywords: YOUNG PIGS, BIOCHEMICAL PARAMETERS OF BLOOD SERUM, MUSCLE TISSUE, PHYSICAL AND CHEMICAL PROPERTIES OF THE  M. LONGISSIMUS DORSI, CORRELATION

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ANALYSIS OF THE DYNAMICS OF ANTIBODY SYNTHESIS IN HORSE SERUM AFTER VACCINATION WITH UA-07 “ANTRAVAK” AGAINST ANTHRAX OF BACILLUS ANTHRACIS STRAIN

A. Rublenko¹, V. G. Skripnik²

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¹Bila Tserkva National Agrarian University,
126 Stavyschanska str., Bila Tserkva 09117, Ukraine

²National Research Institute laboratory diagnostics and veterinary and sanitary examination,
30 Donetska str., Kyiv 03151, Ukraine

The results of research to determine the level of antibody titer of anthrax in horses of all ages after a vaccine against anthrax strain of animals Bacillus anthracis UA–07 “Antravak” are presented in the paper. For the research three groups of horses were formed. All research groups of animals received vaccine at a dose of 1.0 cm³. The animals which received saline instead of the vaccine were used as control. The aim of research was to study the dynamics of anthrax formation of antibodies in the serum of horses immunized with the new vaccine against anthrax of domestic animals with a strain of Bacillus anthracis UA–07 “Antravak”. Dynamics of changes was determined by individual selection and study of serum samples by indirect clumping together red blood cells. Animals differed in age, which probably led to different results in antibody synthesis.

The presence of antibody titers to the pathogen Bacillus anthracis horses was established in all age groups of experimental animals. As a result of clinical trials it was found that a vaccine against anthrax strain of animals Bacillus anthracis UA–07 “Antravak” was well tolerated by animals without causing adverse reactions. With the introduction of the recommended dose and route of administration a significant increase of antibodies in horses of 3^rd group compared to other groups and control was noted.

According to the research it was found that the vaccine strain of anthrax animals Bacillus anthracis UA–07 “Antravak” leads to higher antibody titers of anthrax, especially in older animals. The lowest level of antibody synthesis was noted in the 1^st group with the least age of animals.

Keywords: ANTHRAX, DISTRIBUTION, VACCINES, EQUINE, CREDITS, ANTIBODIES, BACILLUS ANTHRACIS, “ANTRAVAK”

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