Bìol. Tvarin, 2017, volume 19, issue 4, pp. 83–87
ANTIBACTERIAL ACTIVITY OF COMPLEX OF ENROFLOXACIN WITH NANOPOLYMER GLULA-DPG-PEG600
O. Chekh1, I. A. Dron2, S. I. Vynnytska2, V. V. Oleksa2, I. E. Atamaniuk3, V. V. Vlizlo1
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1Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine
2National University “Lviv Polytechnic”,
12 Bandery str., Lviv 79013, Ukraine
3State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine
This article presents the results of the study of antibacterial activity of complex of enrofloxacin with nanopolymer based on pseudo-polyamino acids GluLa-DPG-PEG600. Nanopolymer GluLa-DPG-PEG600 has low toxicity to mammalian cells and white laboratory rats, and has an ability to bind blood serum albumins which is important characteristics of nanopolymer as drug transporter.
Antibacterial activity was investigated used the method of serial dilutions which was prepared on microbial cells of Pseudomonas aeruginosa incubated at 37 °C for 44 hours. After 22 and 44 hours of incubation we calculated the density of microbial cells suspension. The results of the study have shown that the complex of enrofloxacin with GluLa-DPG-PEG600 has higher antibacterial activity that enrofloxacin (control). The minimum inhibitory concentration (MIC) of complex after 22 hours of incubation was 3.12 μg/ml and was lower that control group (6.25 μg/ml). However, after 44 hours of incubation MIC of the complex of enrofloxacin with GluLa-DPG-PEG600 was 6.25 μg/ml and was the same as MIC of enrofloxacin. Higher antibacterial activity of the complex of enrofloxacin with GluLa-DPG-PEG600 compared with the control is probably due to the presence of polyethylene glycol and lauric acid in GluLa-DPG-PEG600 molecule which can acts as membrane permabilizers and particularly PEG can enhance antibiotic affinity to DNA that probably increase its antibacterial activity.
Obtained results show positive prospects for using nanopolymer GluLa-DPG-PEG600 as antibiotic transporter, particularly enrofloxacin which belong to fluoroquinolones class of antibiotics.
Antibacterial activity was investigated used the method of serial dilutions which was prepared on microbial cells of Pseudomonas aeruginosa incubated at 37 °C for 44 hours. After 22 and 44 hours of incubation we calculated the density of microbial cells suspension. The results of the study have shown that the complex of enrofloxacin with GluLa-DPG-PEG600 has higher antibacterial activity that enrofloxacin (control). The minimum inhibitory concentration (MIC) of complex after 22 hours of incubation was 3.12 μg/ml and was lower that control group (6.25 μg/ml). However, after 44 hours of incubation MIC of the complex of enrofloxacin with GluLa-DPG-PEG600 was 6.25 μg/ml and was the same as MIC of enrofloxacin. Higher antibacterial activity of the complex of enrofloxacin with GluLa-DPG-PEG600 compared with the control is probably due to the presence of polyethylene glycol and lauric acid in GluLa-DPG-PEG600 molecule which can acts as membrane permabilizers and particularly PEG can enhance antibiotic affinity to DNA that probably increase its antibacterial activity.
Obtained results show positive prospects for using nanopolymer GluLa-DPG-PEG600 as antibiotic transporter, particularly enrofloxacin which belong to fluoroquinolones class of antibiotics.
Keywords: NANOPOLYMERS, PSEUDO-POLYAMINO ACIDS, ANTIBIOTICS, ENROFLOXACIN, MICROBIAL CELLS, ANTIBACTERIAL ACTIVITY
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