The Animal Biology

HISTOSTRUCTURE OF INTERNAL ORGANS IN FEMALE RATS OF FIRST GENERATION FOR DIFFERENT DOSES OF GERMANIUM CITRATE

M. I. Khrabko¹, R. S. Fedoruk¹, Yu. V. Martyn¹, U. I. Tesarivska², M. I. Shumska²

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¹ Institute of Animal Biology NAAS,
38 Vasyl Stus str., Lviv 79034, Ukraine

²State Scientific-Research Control Institute
of Veterinary Medicinal Products and Feed Additives,
11 Donetska str., Lviv 79019, Ukraine

The structural differences of the spleen, thymus, liver and lymph nodes of female rats of first generation (F₁) under the influence of various doses of nano-germanium citrate (NGeCit) have been studied. The experimental part of the work was performed on the F₁ female rats which were watered various quantities of germanium citrate, obtained by the method of nanotechnology. Female research groups, in contrast to the control (I), were given 10 (II), 20 (III) and 200 (IV) mg Ge/kg of body weight from the period of drinking water consumption, during growth, development and pregnancy. Tissue samples of F₁ female in control and experimental groups were taken on the 21^st day of pregnancy.

Morphostructural changes of internal organs were determined using standard methods of morphological and histological studies which revealed some differences in action of different doses of NGeCit in F₁ females of research groups compared with the control. Histostructural changes of hepatocyte size with their increase, deformation of veins and stratification of stroma around them in F₁ animals at the action of 20 μg Ge/kg of body mass were established. The action of 200 μg Ge/kg of body weight in F₁ females caused a violation of the organ hemodynamics and dilatation of the walls of its vessels. The use of 10 and 20 μg Ge/kg body weight in F₁ females led to an improvement in the morphofunctional state and histostructural characteristics of the thymus, whereas 200 μg Ge/kg body weight caused moderate stromal edema and narrowing of the cortical zone of this organ.

The established differences in the morphostructure of the internal organs of F₁ female rats for prolonged action of NGeCit may be determined by the dose of its application. More pronounced morphological and histological changes are noted in the organs of F₁ females for the action of 200 μg Ge/kg of body weight.

Keywords: RATS, GERMANIUM CITRATE, HISTOSTRUCTURE, ORGANS

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