Bìol. Tvarin, 2017, volume 19, issue 4, pp. 58–63


A. Rublenko1, V. G. Skripnik2

This email address is being protected from spambots. You need JavaScript enabled to view it.

1Bila Tserkva National Agrarian University,
126 Stavyschanska str., Bila Tserkva 09117, Ukraine

2National Research Institute laboratory diagnostics and veterinary and sanitary examination,
30 Donetska str., Kyiv 03151, Ukraine

The results of research to determine the level of antibody titer of anthrax in horses of all ages after a vaccine against anthrax strain of animals Bacillus anthracis UA07 “Antravak” are presented in the paper. For the research three groups of horses were formed. All research groups of animals received vaccine at a dose of 1.0 cm3. The animals which received saline instead of the vaccine were used as control. The aim of research was to study the dynamics of anthrax formation of antibodies in the serum of horses immunized with the new vaccine against anthrax of domestic animals with a strain of Bacillus anthracis UA07 “Antravak”. Dynamics of changes was determined by individual selection and study of serum samples by indirect clumping together red blood cells. Animals differed in age, which probably led to different results in antibody synthesis.

The presence of antibody titers to the pathogen Bacillus anthracis horses was established in all age groups of experimental animals. As a result of clinical trials it was found that a vaccine against anthrax strain of animals Bacillus anthracis UA07 “Antravak” was well tolerated by animals without causing adverse reactions. With the introduction of the recommended dose and route of administration a significant increase of antibodies in horses of 3rd group compared to other groups and control was noted.

According to the research it was found that the vaccine strain of anthrax animals Bacillus anthracis UA07 “Antravak” leads to higher antibody titers of anthrax, especially in older animals. The lowest level of antibody synthesis was noted in the 1st group with the least age of animals.


  1. Bahl I. M., Rosenberg K. High abundance and diversity of Bacillus anthracis plasmid pXO1-like replicons in municipal wastewater. Journal of Microbiol Ecol., 2010, no. 74. pp. 241–247.
  2. Dangerous situation medico-biological nature and measures to minimize their negative consequences. National Report on the State of Techno and Natural Safety in Ukraine in 2012. 2013, pp. 65–66. Available at: www.mns.gov.ua/files/proznos/report/2012/2.2012.pdf.
  3. Grinevich O. Y., Markovic I. G., Markovic I. F. Role of monitoring infections common to humans and animals, to ensure biosafety population of Ukraine. Ukrainian Medical Journal, 2012, no. 5, pp. 111–114.
  4. Kobiler D., Gozes Y., Rosenberg H., Marcus D., Reuveny S. Efficiency of protection of guinea pigs against infection with Baccilus anthracis spore by passive immunization Infect. Immun., 2002, vol. 70, no. 2, pp. 544–560. https://doi.org/10.1128/IAI.70.2.544-550.2002
  5. Liman O. Yu., Murtazayeva L. A., Klee S., Liman A. P. Molecular technologies anthrax detection by PCR of different formats. Journal of Biotechnology, 2013, no. 3, pp. 86–96.
  6. Ruden V. V., Moskvyak E. V., Banchuk N. V. Legislative and regulatory adjustment process of organizing and conducting vaccination of the population in Ukraine. The guidelines. Kyiv, 2011, 105 p.
  7. Schmitt K. K., Meysik K. S., O’Braen A. D. Bacterial Toxins: Friends or Foes? Clinical Microbiology and Antimicrobial Chemotherapy, 2000, vol. 2, no. 1, pp. 4–15.
  8. Skrypnyk V., Golovko A., Skrypnyk A., Rublenko I. Dynamics of anthrax cases in Ukraine during 1970–2013 years. International journal of infectious diseases, 2014, no. 21, pp. 181. https://doi.org/10.1016/j.ijid.2014.03.798
  9. Syurin V. N., Belousov R. V., Fomina N. V. Veterinary Virology: a textbook. Accomplice. Moscow, Kolos, 1984, pp. 359–361.
  10. Tarasov V. V. Transmissible zooantroponozy. Journal of RAB-info. Available at: https://does.google.com.document/d/1x0z7xq1TZR%JR7dxFO.
  11. Toby I. T., Widmer J., Dyer D. W. Divergence of protein-coding capacity and regulation in the Bacillus cereus sensu lato group. The BMC Bioinformatics, 2014, vol. 15, no. 11. Available at: http://www.biomedcentral.com/1471–2105/15/S11/S8. https://doi.org/10.1186/1471-2105-15-S11-S8
  12. Terentev F. A. Anthrax and animals struggle with neu. Moscow, OGIZ — Selhozhyz State Publishing of agricultural literature, 1946, 63 p.
  13. Woods C. W., Ospanov K., Myrzabekov A. Risk factors for human anthrax among contacts of antrax-infected livestock in Kazakhstan. Journal of the American society of tropical me dicine and hygiene, 2004, vol. 71, no. 1, pp. 48–52.
  14. Zwick M. E., Joseph S. J., Didelot X. Genomic characterization of Bacillus cereus sensu lato species: backdrop to the evolution of Bacillus anthracis. The Genome Res., 2012, vol. 3, no. 29, pp. 1512–1524. https://doi.org/10.1101/gr.134437.111

Download full text in PDF format