Bìol. Tvarin, 2017, volume 19, issue 4, pp. 9–15


О. Buchko, A. Pylypets

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article is devoted to the problem of improving the adaptive capacity of young highly productive animals using biologically active substances under intensive technologies during one of the critical periods of their development — weaning from sows. For this purpose, the effect of formic acid and dietary feed supplement “Humilid” on the free radical processes and antioxidant defense system of the piglet’s organism during the first 40 days from the birth was studied.

It was found that the addition of formic acid (2nd experimental group) and its complex with humic supplement (1st experimental group) to the standard ration for a month from 10- to 40-day age (18 days before and 12 days after weaning from sows) is accompanied by reducing the concentration of oxidative stress products. A reduction of the content of TBA-active products, lipid hydroperoxides and carbonyl groups of proteins in the animals of the experimental group was observed. It was established an increase of the parameters of antioxidant defense system in the piglet’s organism — the activity of superoxide dismutase and catalase in erythrocytes of the animals of the first experimental group under the administration of the complex additives. Therefore, combined action of organic acid and humic supplement most effectively manifested their antioxidant effect in the blood of piglets during critical periods of their life — 20 days of life and 2 days after weaning from sows (30 days of life).

It was concluded that it is recommended to add the complex of bioactive feed supplement “Humilid” and formic acid to the standard diet of piglets to activate their adaptive capacity and reduce stress factors in animals in the critical periods of their ontogenesis.


  1. Ahmed S. T., Hwang J. A., Hoon J. Comparison of Single and Blend Acidifiers as Alternative to Antibiotics on Growth Performance, Fecal Microflora and Humoral Immunity in Weaned Piglets. Asian-Aust. J. Anim Sci., 2014, vol. 27, no. 1, pp. 93–100. https://doi.org/10.5713/ajas.2013.13411
  2. Avvakumova N. P. Humin substances — the factor of biosystems protection from ecotoxicogenic substances. Izv. of the SSC of the RA of Sciences, 2009, vol. 11, no. 1 (2), pp. 197–201. (in Russian)
  3. Bittner M. Direct effects of humic substances on organisms. Brno, Czech Republic, 2006, 31 р.
  4. Canibe N., Miettinen H., Jensen B. Effect of adding Lactobacillus plantarum or a formic acid containing-product to fermented liquid feed on gastrointestinal ecology and growth performance of piglets. Livest. Sci., 2008, vol. 114, pp. 251–262. https://doi.org/10.1016/j.livsci.2007.05.002
  5. Dong Yong Kil, Woong Bi Kwon, Beob Gyun Kim. Dietary acidifiers in weanling pig diets: a review. Colombian journal of animal science and veterinary medicine, 2011, vol. 24, no. 3, pp. 231–247.
  6. Eidelsburger U., Wald C., Portocarero-Khan N. Effect of potassium diformate (Formi®) inclusion at two different dietary protein levels on performance and health of weaner piglets. Proceedings of the 6th BOKU Symposium Tierernährung, Vienna (Austria), 2007, pp. 248–253.
  7. Li Z., Yi G., Yin J. Effects of organic acids on growth performance, gastrointestinal pH, intestinal microbial populations and immune responses of weaned pigs. Asian-Aust. J. Anim. Sci., 2008, vol. 21, pp. 252–261.
  8. Lückstädt C. Effects of dietary potassium diformate on feed intake, weight loss and backfat reduction in sows: pre-farrowing till weaning. Advances in Animal Biosciences, 2011, vol. 2, no. 1, pp. 145.
  9. Lückstädt C., Mellor S. The use of organic acids in animal nutrition, with special focus on dietary potassium diformate under European and Austral-Asian conditions. Recent Advances in Animal Nutrition, 2011, vol. 18, pp. 123–130.
  10. Metzler B., Mosenthin R. Effects of organic acids on growth performance and nutrient digestibilities in pigs. Nottingham University Press, Nottingham (UK); Acidifiers in Animal Nutrition, 2007, pp. 39–54.
  11. Mikhaylenko E., Dyomshina O., Stepchenko L. Antioxidant system of liver in cross Cobb-500 broilers at additions of natural biologically active supplements based on humic substances. News of DSAEU, 2016, no. 4 (42), pp. 120–125. (in Ukrainian)
  12. Mroz Z. Organic acids as potential alternatives to antibiotic growth promoters for pigs. Advances in Pork Production, 2005, vol. 16, pp. 169–182.
  13. Nurten G., Umit P., Hakan B. Effects of supplemental humic acid on ruminal fermentation and blood variables in rams. Ital. J. Anim. Sci., 2010, vol. 9, no. 74, pp. 390–393.
  14. Pejsak Z. Choroby swin. Poznan, Pol. Wyd. Rol., 2002, 353 p. (in Polish)
  15. Ratriyanto A., Eklund M., Jezierny D. Associated effects of betaine, organic acids and inulin on nutrient digestibilities and intestinal microbial fermentation characteristics in piglets. Proceedings of the 17th Intern. Scientific Symposium on Nutrition of Domestic Animals, 2008, vol. 17, pp. 209–218.
  16. Suiryanrayna M. V., Ramana J V. A review of the effects of dietary organic acids fed to swine. Journal of Animal Science and Biotechnology, 2015, pp. 1–11. https://doi.org/10.1186/s40104-015-0042-z
  17. Trckova M., Matlova L., Hudcova H. Peat as a feed supplement for animals: a review. Vet. Med., 2005, vol. 50, no. 8, pp. 361–377. https://doi.org/10.17221/5635-VETMED
  18. Upadhaya S. D., Lee K. Y., Kim I. H. Protected Organic Acid Blends as an Alternative to Antibiotics in Finishing Pigs. Asian-Aust. J. Anim. Sci., 2014, vol. 27, no. 11, pp. 1600–1607.
  19. Vlizlo V. V., Fedoruk R. S., Ratych I. B. Laboratory methods of research in biology, animal husbandry and veterinary medicine. Lviv, Navy, 2012, 764 p. (in Ukrainian)
  20. Vondruskova H., Slamova R., Trckova M. Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review. Veterinarni Medicina, 2010, vol. 55, no. 5, pp. 199–224. https://doi.org/10.17221/2998-VETMED
  21. Weber T. E., van Sambeek D. M., Gabler N. K. Effects of dietary humic and butyric acid on growth performance and response to lipopolysaccharide in young pigs. J. Anim. Sci., 2014, vol. 92, pp. 4172–4179. https://doi.org/10.2527/jas.2013-7402
  22. Yarovan N. I. Biochemical aspects of the evaluation, diagnosis and prevention of technological stress in farm animals. Dr. biol. sci. diss. Moscow, 2008, 41 p. (in Russian)
  23. Yin J., Wu M. M., Xiao H., Ren W. K., Duan J. L., Yang G., Li T. J., Yin Y. L. Development of an antioxidant system after early weaning in piglets. J. Anim. Sci., 2014, vol. 92, pp. 612–619. https://doi.org/10.2527/jas.2013-6986
  24. Zhukova I. O. Effect of oxidative stress on animals body. Scientific Messenger of LNUVMB named after S. Z. Gzhytsky, 2013, vol. 15, no. 3 (57), pp. 83–87. (in Ukrainian)

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