Bìol. Tvarin, 2018, volume 20, issue 2, pp. 65–70


R. A. Pelenio
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Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50 Pekarska str., Lviv 79010, Ukraine

The article is devoted to the study of the actual problem of influence of the swine lesion by the association of nematodes, the protozoa and mixed nematode and protozoa invasion on the indices of nonspecific immunity. For this purpose, the intact and nematodes affected by the association (ascaris + trichuruses + esophagosomes), protozoa (balantidium + isosporis + eimeria) and mixed nematode and protozoa invasion (ascaris + balantidium + eimeria) of piglets of 2–3 months old of large white breed were investigated.

It was determined that the presence in piglets’ organisms of the associations of intestinal parasites leads to decrease of the humoral protection indicators. In blood serum of animals, there was a significant decrease of the lysozyme and lymphocytic activity indicators compared to control group, as well as the growth of complementary activity at protozoa and mixed invasions. The bactericidal activity of serum in piglets was significantly lower in comparison with control group in all investigated invasions. Data are given on reduction of phagocytic activity of neutrophils, phagocytic number, phagocytic index and oxidative-reduction activity of neutrophil granulocytes in piglets at associated invasions.

On the basis of the obtained results it has been concluded that in the presence of the intestinal parasites in piglets organism there is a decrease of bactericidal, lysozyme, phagocytic activity of neutrophils, phagocytic index and phagocytic number and growth of complementary activity. The probable reduction of bactericidal activity of neutrophils, as compared to control, is found in piglets infested with the protozoa (P<0.01), nematodes (P<0.05) and their association (P<0.01), and bactericidal, lysozyme, phagocytic activity of neutrophils and lysozyme and phagocytic activity of neutrophils — for the defeat of piglets by mixed protozoa (P<0.05) and nematode-protozoa invasion (P<0.01). The difference between the piglets complementary activity was probable compared with the control, for mixed protozoa and nematode-protozoa invasion, respectively, made up 8.7 % (P<0.05) and 9.6 % (P<0.01). The decrease in ill animals of phagocyte protection indicators, in comparison with the control group, indicates on the development of immunodeficiency, intoxication of the body, damage to the gastrointestinal tract and metabolic disorders, as well as the low degree of activation of oxygen-dependent mechanisms of killing of unactivated phagocytes and the development of chronic infectious process.


  1. Bradley J. E., Jackson J. A. Immunity, immunoregulation and the ecology of trichuriasis and ascariasis. Parasite Immunology, 2005, vol. 26, no. 11–12, pp. 429–441.
  2. Clinical Immunology. Textbook for students of medical universities. Ed. by A. V. Karaulov. Moscow, Medical News Agency, 1999, 604 p. (in Russian)
  3. Frontera E., Roepstorff A., Gazquez A. Immunohistochemical distribution of antigens in liver of infected and immunized pigs with Ascaris suum. Vet. Parasitol., 2003, vol. 111, no. 1, pp. 9–18. https://doi.org/10.1016/S0304-4017(02)00347-3
  4. Kolobkova N. M., Krygina E. A. Dynamics of hematological and some biochemical indices of pigs with ascariasis. Mats-IV IV scientific-practical conference. Troitsk, 2000, pp. 17–18. (in Russian)
  5. Lewis R., Behnke J. V., Cassidy J. P. The migration of Ascaris suum larvae, and the associated pulmonary inflammatory response in susceptible C57BL/6j and resistant CBA/Ca mice. Parasitology, 2007, vol. 134, no. 9, pp. 1301–1314. https://doi.org/10.1017/S0031182007002582
  6. Mazmanyan M. V. Parasitic pathogens: allergens, triggers or inhibitors of allergy. Quality of life. Medicine, 2005, no. 4 (11), pp. 49–52. (in Russian)
  7. Mejer H., Roepstorff A. Ascaris suum infections in pigs born and raised on contaminated paddocks. Parasitology, 2006, vol. 133, no. 3, pp. 305–312. https://doi.org/10.1017/S0031182006000394
  8. Moskalev A. A., Sydorenko S. V. Influence of conditions of calves growing in the early post natal period on their natural resistance and productivity. Act. problems of intensive livestock development: Col. of scientific works, 2007, in. 10, no. 2, pp. 171–177. (in Russian)
  9. Riggin A. J., Brewer M. B., Turner P. L. Paralytic ileus secondary to intestinal ascariasis. Am Surg., 2012, vol. 78, no. 11, pp. 481–483.
  10. Saitbatalova A. I., Mannapova P. T. Immunodeficiencies of dogs on the background of invasive diseases. Apitherapy today with a biological pharmacy bee in the XXI century. Ufa, 2000, pp. 315–316.
  11. Sapin M. R., Nikitiuk D. B. Immune system, stress and immunodeficiency. Moscow, APP “Dzhangar”, 2000, 184 p. (in Russian)
  12. Shabaev V. A., Pronin N. M. Problems of general and regional parasitology. Ulan-Ude, 2000 p. 26–30. (in Russian)
  13. Silva S. R., Jacysyn J. F., Macedo M. S., Faquim-Mauro E. L. Immunosuppressive components of Ascaris suum down-regulate expression of costimulatory molecules and function of antigen-presenting cells via an IL-10-mediated mechanism. European Journal of Immunology, 2006, vol. 36, pp. 3227–3237. https://doi.org/10.1002/eji.200636110
  14. Souza V. M., Jacysyn J. F., Macedo Souza M. S. IL-4 and IL-10 are essential for immunosuppression induced by high molecular weight proteins from Ascaris suum. Cytokine, 2004, vol. 28, no. 2, pp. 92–100. https://doi.org/10.1016/j.cyto.2004.07.004
  15. Vasiliev E. H., Arkhipov I. A. Fertility of Ascaris suum in pigs’ organisms. Mat. scientific reports Conf.: Theory and practice of combating parasitic diseases, 2002, is. 3, pp. 71–73 (in Russian)
  16. Vlizlo V. V., Fedorchuk R. S., Ratych I. B. Laboratory methods of research in biology, livestock and veterinary medicine. A reference book, ed. by V. V. Vlizlo. Lviv, Spolom, 2012, 764 p. (in Ukrainian)
  17. Yakubovskyi M. V., Dlubada V. I. Nematodoses of horses in Belarus. Veterinary Medicine, 2005, no. 1, pp. 34–36. (in Russian)
  18. Zakharchuk O. I. Pathomorphological changes in the host’s organism as a result of the migration of the larval stages of helminths. Clinical Anatomy and Operative Surgery, 2007, vol. 6, no. 4, p. 87–91. (in Ukrainian)

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