Bìol. Tvarin, 2018, volume 20, issue 3, pp. 77–83

TRANSFUSION OF CANINE CRYOPRESERVED RED BLOOD CELLS IN TREATMENT OF BABESIOSIS

О. А. Pervushina, ONDenysova, GFGegunov, NIGladka, VOPrichodchenko, TIYakimenko

This email address is being protected from spambots. You need JavaScript enabled to view it.

Kharkiv state zooveterinary academy, HDZVA,
Mala Danylivka, Derhachivsky district, Kharkiv region, 62341, Ukraine

The results of the evaluation of the clinical and biochemical efficiency of dogs cryopreserved red blood cells transfusion with acute form of babesiosis are presented. Cryopreserved erythrocyte mass was obtained by rapid immersion of red blood cells in liquid nitrogen with subsequent heating in a water bath. As a cryoprotectant, a 17.5 % solution of hydroxyethyl starch 200 was used. Research studies were on three groups of animals control and two experimental (animals with acute form of babesiosis and basic drug treatment; animals with acute form of babesiosis, basic drug treatment and transfusion of cryopreserved erythrocytes).

It has been established that the application of cryopreserved erythrocyte transfusion leads to an increase in the level of hematocrit and hemoglobin, as well as the red blood cell count in 24 hours. On the 10th day these indices were close to the control values and almost twice exceed the hematological parameters in animals with basic treatment. Biochemical parameters of blood serum of dogs with babesiosis pointed out uremia, high creatinine level, and an increased activity of liver test enzymes at the first day of the disease. Ten days after the basic course of therapy in combination with hemotransfusion of cryopreserved erythrocytes, in patient dogs there is a normalization of the activity of alanine aminotransferase, the amount of bilirubin and urea. The amount of creatinine and the activity of aspartate aminotransferase and lactate dehydrogenase in this group of experimental animals return to the reference levels.

It was shown that transfusion of cryopreserved erythrocyte mass is highly effective in treating dogs for the acute course of babesiosis and contributes to the normalization of hematological and biochemical parameters.

Keywords: DOGS, RED BLOOD CELLS, CRYOPRESERVATION, BABESIOSIS, TRANSFUSION, HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS

  1. Akbaev M. Sh., Vodyanov A. A., Kosminkov N. E., Yatusevich A. I., Pashkin P. I., Vasilevich F. I. Parasitology and invasive diseases of animals. Moscow, 2002, 743 p. (in Russian)
  2. Couto C. G., Iazbik M. C. Effects of blood donation on arterial blood pressure in retired racing Greyhounds. J. Vet. Intern. Med., 2005, vol. 19, issue 6, pp. 845–848. https://doi.org/10.1111/j.1939-1676.2005.tb02775.x
  3. Denisova O. N. Cryosensitivity of erythrocytes in different mammalian species. PhD of biological sci. diss., Kharkiv, 2006, 169 p. (in Russian)
  4. Feldman B. F., Kristensen A. T. Modern veterinary blood banking practices and their applications in companion animal practice. Vet. Clin. North. Am. Small. Anim. Pract., 1995, vol. 25, issue 6, pp. 1231–1243. https://doi.org/10.1016/S0195-5616(95)50151-2
  5. Hundfeld K. P., Hildebrandt A., Gray J. S. Babesiosis: recent insights into an ancient disease. Int. J. Parasitol., 2008, vol. 38, issue 11, pp. 1219–1237. https://doi.org/10.1016/j.ijpara.2008.03.001
  6. Henrich H. A., Langer R. Erythrocytes after cryopreservation with HES: molecular, structural and functional characteristics. Zentralbl. Chir., 1999, vol. 124, no. 4, pp. 271–277.
  7. Kaneko J. J., Harvey J. W., Bruss M. Clinical biochemistry of domestic animals. New York, Academic Press, 1989, 932 p.
  8. Kim H., Tanaka S., Une S., Nakaichi M., Sumida S., Taura Y. A comparative study of the effects of glycerol and hydroxyethyl starch in canine red blood cell cryopreservation, J. Vet. Med. Sci., 2004, vol. 66, issue 12, pp. 1543–1547. https://doi.org/10.1292/jvms.66.1543
  9. Leisewitz A. L., Guthrie A. J., Berry W. L. Evaluation of the effect of whole-blood transfusion on the oxygen status and acid-base balance of Babesia canis infected dogs using the oxygen status algorithm. J. S. Afr. Ve.t Assoc., 1996, vol. 67, no. 1, pp. 20–26.
  10. Sputtek A., Langer R., Schmid H., Steigerwald R., Trenkel K., Kron W., Henrich H. A., Körber C., Rau G. Cryopreservation of erythrocytes with hydroxyethyl starch. In vitro results leading to an autologous retransfusion model in the dog. Beitr. Infusionsther., 1992, vol. 30, pp. 292–296.
  11. Zhegunov G. F., Denisova O. N. Permeability of mammalian erythrocytes for the molecules of glycerol and DMSO and the level of cellular viability after freezing-thawing. Dopov. Nac. akad. nauk. Ukr., 2010, no. 12, pp. 139–143. (in Ukrainian)

Download full text in PDF

Search