The Animal Biology

THE CONDITION OF THE PROOXIDANT-ANTIOXIDANT SYSTEM IN SKELETAL MUSCLES OF EXPERIMENTALLY DIABETIC RATS UNDER VANADIUM CITRATE EFFECT

R. Iskra, H. Klymets, O. Sushko, L. Ponkalo, O. Svarchevska

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The effect of vanadium citrate on the content of lipid peroxide oxidation products, reduced glutathione, the activity of the antioxidant system enzymes and glucose-6-phosphate dehydrogenase in the thigh skeletal muscles of alloxan-induced diabetic rats was studied. Animals were divided into five groups: I group — control, II, III, IV and V — experimental. The rats of I and II groups were given pure water to drink, whereas a solution of vanadium citrate at concentrations of 0.125, 0.5 and 2.0 μg V/ml of water was added to drinking water of III, IV and V groups within a month. The animals of all experimental (II–V) groups were induced diabetes by intraperitoneal injection of alloxan (150 mg/kg body weight).

In muscles of the II group experimentally diabetic animals, the increase in the content of hydroperoxides and TBA-active products and the decrease in the activity of antioxidant protection enzymes (superoxide dismutase, catalase and glutathione reductase), glucose-6-phosphate dehydrogenase and the content of reduced glutathione were observed, while the activity of glutathione peroxidase increased as compared to control animals. Under watering vanadium citrate at different concentrations, there was a decrease in the content of hydroperoxides (in III, IV and V groups) and TBA-active products (in group III), but the increase in the activity of superoxide dismutase and catalase (in group V), glutathione reductase (in III, IV and V groups), glucose-6-phosphate dehydrogenase (in IV and V groups), and the content of reduced glutathione (in group V), while glutathione peroxidase activity decreased (in III, IV and V groups), as compared to the muscle indexes in experimentally diabetic animals of the II group. Vanadium citrate at the concentrations under investigation has a dose-dependent stabilizing effect on the condition of the prooxidant-antioxidant system in skeletal muscles of experimentally diabetic rats.

Keywords: VANADIUM CITRATE, ANTIOXIDANT SYSTEM, DIABETES, SKELETAL MUSCLES, RATS

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