The Animal Biology

INFLUENCE OF CHROMIUM CITRATE ON ANTIOXIDANT DEFENSE IN THE LIVER OF RATS WITH EXPERIMENTALLY INDUCED DIABETES

O. O. Sushko^1,2, R. Ya. Iskra¹, V. I. Pryimych²

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¹Institute of animal biology NAAS,
38 V. Stusa str., Lviv 79034, Ukraine

²Lviv National University of the Veterinary Medicine and Biotechnologies named after S. Z. Gzhytsky,
50, Pekarska str, Lviv 79010, Ukraine

We have investigated the activity of the antioxidant system and the level of peroxide oxidation of lipids in the liver of rats with aloxane-induced diabetes and influence of chromium citrate, which was given to animals during one month in an amount of 0.1 and 0.2 mg/ml of water. Diabetes mellitus was experimentally induced by intraperitoneal injection of 5 % solution of alloxan monohydrate in an amount of 150 mg/kg body weight. Diabetes was detected by measuring glucose levels in blood collected from the tail vein. On the 40^th day of the experiment, after the decapitation of the animals, the liver tissue for research was collected.

Content of lipid hydroperoxides and TBA-active products significantly increased in the rat liver homogenate of the control diabetic group. Content of lipid hydroperoxides and TBA-active products decreased in the liver of rats watered by chromium citrate in the amount of 0.1 mg/ml compared to corresponding levels in the diabetic control group.

The activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase and the content of reduced glutathione significantly decreased in the liver of animals of the control diabetic group. However, under the effect of chromium citrate in the amount of 0.1 and 0.2 mg/ml of water those indications normalized, namely, the activity of catalase, superoxide dismutase, glutathione peroxidase increased and the content of reduced glutathione increased, compared to their levels in the liver of animals in the diabetic control group.

Probable changes in the content of products of lipid peroxidation and the activity of enzymes in the antioxidant system in the liver are most noticeable when using chromium citrate in the amount of 0.1 mg/ml of water. These indexes show the normalization of antioxidant protection for the effects of chromium citrate in tissues of rats with experimentally induced diabetes.

Keywords: RATS, DIABETES, ALLOXAN, LIVER, ANTIOXIDANT DEFENSE, LIPID PEROXIDATION

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