The Animal Biology

INFLUENCE OF CHLORPYRIFOS ON SOME PHYSIOLOGICAL AND BIOCHEMICAL INDICES OF DANIO RERIO

V. V. Dovhanyuk, V. P. Rosalovsky, Yu. T. Salyha

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

Physiological and biochemical parameters of Danio rerio fish with toxic injury by chlorpyrifos, one of the most well-known organophosphorus compounds, an active substance of many pesticides were studied.

The studies were conducted on adult Danio rerio fishes with an average weight of 0.6 g per 10 individuals per group. To determine the toxicity of chlorpyrifos, the experimental groups of fishes were placed in 5 l aquariums for 96 hours with water in which chlorpyrifos was added in the range of concentrations of 0.375–2 mg/l. Biochemical studies were carried out in blood plasma, which was taken from fish at different concentrations and duration of exposure to the xenobiotic.

The parameters of the acute toxicity of chlorpyrifos for Danio rerio were determined. With experiment duration of 96 hours, the maximum concentration of chlorpyrifos, in which the death of D. rerio fish was not fixed, was 0.375 mg/l, the absolute lethal concentration was 2 mg/l, half-lethal concentration (LC50) was 1.23 mg/l.

Danio rerio intoxication by selected concentrations and duration of chlorpyrifos was accompanied by a decrease of cholinesterase activity in blood plasma; at the influence of chlorpyrifos at the concentration of 1 mg/l, an increase in the number of hemolysed erythrocytes was observed at 16.5 % (P<0.05), which may indicate a structural damage to the protein-lipid matrix of erythrocytes membranes, while the duration of hemolysis of erythrocytes of experimental fish compared to control did not undergo significant changes.

The obtained results prove the efficiency of the use of Danio rerio fish as a biological model for toxicological studies of organophosphorus compounds, in particular chlorpyrifos.

Keywords: CHLORPYRIPHOS, DANIO RERIO, INTOXICATION, CHOLINESTERASE, BLOOD

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