The Animal Biology

DYNAMICS OF NON-SPECIFIC RESISTANCE IN PIGS AFTER ACTION OF NEW IMMUNOTROPIC DRUGS

S. I. Fedoryshyn, I. O. Matiukha, N. A. Broda, D. I. Mudrak

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Institute of Animal Biology NAAS,
38 V. Stus str., Lviv 79034, Ukraine

The article presents the results of research on the influence of new immunotropic drugs containing piperidine acetate on the status of the cellular and humoral links of the non-specific resistance in piglets during the process of their cultivation. A comparative analysis of the influence of “Trifuzol” drugs and a new immunotropic agent in the form of a liposomal emulsion “Nero” on the dynamics of indicators of the natural resistance of the piglet organism is carried out. The experiment was conducted in three groups of pigs, analogues by body weight and sex, for 4 animals in each group. For pigs on the 2^nd and 14^th day of age, an intramuscular dose of 1 ml per animal was introduced: 1^st experimental group — Trifuzol 1 %; control group — isotonic solution; the 2^nd experimental group — the new liposomal drug “Nero”. Material for research was the blood of piglets at 2-, 7-, 14- and 26-day-old age. The blood serum was used to determine the bactericidal and lysozyme activity (BA and LA), as well as the content of circulating immune complexes (CIC) and middle mass molecules (AWM); in the blood — phagocytic activity, index and number. In pigs, which were treated by liposomal preparation at the 14^th and 26^th day of life the higher phagocytic activity (PhA) of blood neutrophils was confirmed. Significant changes in the humoral level of non-specific resistance have been found in the pigs for the investigational drugs, as evidenced by a significantly higher bactericidal (BA) and lysozyme activity of the serum (LA), both in terms of the action of the drug Trifuzol and liposomal drag. Developed liposomal preparation “Nero” caused a reliable increase in the intensity of BASK in piglets from the 7^th day of life and until the end of the experiment, and lysozyme activity at 26-day age was 22 % (P<0.01) higher than in control. The applied drugs contributed to a gradual decrease in the concentration of circulating immune complexes (CIC) in the blood of pigs, especially these changes were expressed at 14- and 26-day-old age. In the age dynamics, there was an increase in the content of medium-weight molecules (MSM) in piglets’ blood. The introduction of the liposomal preparation caused a probable decrease in their content in the piglets’ blood at 26 days of age, indicating the inhibitory effect of its factors on the content of products of endogenous intoxication of the body.

Keywords: PIGLETS, BLOOD, HUMORAL FACTORS OF PROTECTION, BACTERICIDAL ACTIVITY, PHAGOCYTAL ACTIVITY, LYSOCIMIC ACTIVITY, IMMUNOMODULATORS

1. Allen T. H., Hansen C. B., Stuart D. D. Targeted sterically stabilized liposomal drug delivery. In: Medical application of liposomes (D. D. Lasic, ed.). Amsterdam, Elsevier, 1998, pp. 297–323. DOI: 10.1016/B978-044482917-7/50018-1.
2. Bessarabov B. F., Bondarev E. I., Stolyar T. A. Poultry farming and technology for the production of eggs and poultry. A textbook for high schools. Saint Petersburg, Lan, 2005, 352 p.
3. Bihdan O. A., Parchenko V. V., Panasenko O. I., Knysh Y. G. Pharmacological aspects of application of furan-derivative 1,2,4-triazole-3-thiol furan derivatives. Current issues in pharmacy and medicine: science and practice, 2016, vol. 3, issue 22, pp. 98–102. DOI: 10.14739/2409-2932.2016.3.78004. (in Ukrainian)
4. Bousenka O. T. Technology of livestock production. Kyiv, Higher education, 2005, 496 p. (in Ukrainian)
5. Broshkov M. M., Vischur O. I., Kychun I. V., Fedoryshyn S. I., Parchenko V. V. Status of the cellular immunity level in puppies in terms of vaccination and the action of a new complex preparation in the form of a liposomal emulsion. Scientific Bulletin of Lviv National University of Veterinary Medicine and Biotechnology named after S. Z. Gzhytsky, Series: Veterinary Science, 2017, vol. 19, issue 82, pp. 166–169. (in Ukrainian)
6. Chumachenko V. Yu., Chumachenko V. V., Pavlenko O. I. Investigation of the immune system. Factors Affecting Animal Resistance. Veterinary Medicine of Ukraine, 2004, vol. 5, pp. 33–37. (in Ukrainian)
7. Dedkova A. I., Sergeeva N. N. Clinic and physiological state of pigs on fattening with densiton content. Scientific messenger Orjol GAU, 2010, vol. 3, pp. 84–87. (in Russian)
8. Foldvari M., Oguejiofor C., Afridi S., Kudel T., Wilson T. Liposome encapsulated prostaglandin E1 in erectile dysfunction: correlation between in vitro delivery through foreskin and efficacy in patients. Urology, 1998, vol. 52, issue 5, pp. 838–843. DOI: 10.1016/S0090-4295(98)00299-4.
9. Garashchuk M. I., Stepchenko L. M. Аpplication of Humilid for prophylaxis of post-weaning stress of piglets. Scientific Herald of Veterinary Medicine, Bila Tserkva, 2010, vol. 6, issue 79, pp. 51–54. Available at: https://nvvm.btsau.edu.ua/sites/default/files/visnyky/vet/veterenari%2079.pdf (in Ukrainian)
10. Gilberti R. M., Joshi G. N., Knecht D. A. The рhagocytosis of crystalline silica particles by macrophages. American Journal of Respiratory Cell and Molecular Biology, 2008, vol. 39, issue 5, pp. 619–627. DOI: 10.1165/rcmb.2008-0046OC.
11. Harbustovsky I. F., Golubev I. O. Handbook of basic zoohygienic and veterinary-sanitary standards for the construction and operation of livestock facilities. Kyiv, Harvest, 1974, 277 p. (in Russian)
12. Ignatova I. D. Pharmacotoxicological properties and therapeutic efficacy of antibacterial drugs based on florfenicol. Dr. biol. sci. diss., Moscow, 2007. (in Russian)
13. Izdepsky V. I., Kirichko B. P., Parhomenko L. I. Prospects of application of preparation triasol (rumasol and trefusol) in veterinary medicine. Scientific Herald of Veterinary Medicine, Bila Tserkva, 2010, vol. 4, issue 76, pp. 62–66. Available at: https://nvvm.btsau.edu.ua/sites/default/files/visnyky/vet/veterenari%2076.pdf (in Ukrainian)
14. Karunsky O. Y., Brazhenko V. Y., Tililuk O. O. Technology of production of combined products for livestock. Grain Products and Mixed Fodders, 2013, vol. 55, issue 2, pp. 25–29. (in Ukrainian)
15. Khaytov R. M., Pinegin B. V. Major controversy about immunotropic drugs. Immunology, 1996, issue 6, pp. 4–9. (in Ukrainian)
16. Knysh E. G., Parchenko V. V., Panasenko A.I. Derivatives of 1,2,4-triazole-3-iltio) acetic acid exhibit antioxidant, hepatoprotective and immunostimulatory activity. Patent UA, no. 87184, 2009. (in Ukrainian)
17. Kolaczkowska E. A., Koziol B., Plytycz B., Arnold B. Inflammatory macrophages, and not only neutrophils, die by apoptosis during acute peritonitis. Immunobiology, 2010, vol. 215, issue 6, pp. 492–504. DOI: 10.1016/j.imbio.2009.07.001.
18. Kovalenko V. F., Pochernyayeva H. M., Pochernyayev W. F. Biologically active substances of protective action in pig breeding. Journal of Agrarian Science, 1995, vol. 10, pp. 60–65. (in Ukrainian)
19. Kyrychko B. P. Pathogenetic ground of a treatment of the animals with inflammatory drugs surgical pathology with antioxidant action. Autoref. dr. vet. sci., National University of Life and Environmental Sciences of Ukraine, Kyiv, 2010, 40 p. (in Ukrainian)
20. Linyk V. S, Parkhomenko L. I., Al Nuri A. Growth of the productivity of guial (Coturnix coturnix japonica) by the synthetic 1,2,4-triazole derivatives. Scientific Gerald of Lviv National University named after S. Gzhytsky, 2011, vol. 13, issue 4 (50), part 3, pp. 173–177. (in Ukrainian)
21. Martyshuk T. V., Guty B. V., Vishchur O. I. Level of lipid peroxidation products in the blood of rats under the influence of oxidative stress and under the action of liposomal preparation “Butaselmevit”. Biological Bulletin of Bogdan Chmelnytsky Melitopol State Pedagogical University, 2016, vol. 6, issue 2, pp. 22–27. (in Ukrainian)
22. Maslikov S. M. Antimicrobial activity of Tryfuzol and Rumosol on Staphilococcus aureus for topical issues veterinary surgery and obstetrics. Ukrainian scientific-practical Internet conference devoted to the 20^th anniversary of the department of surgery and obstetrics Poltava state Agrarian Academy, 2015, 22 p. (in Ukrainian)
23. Nagaevich V. M. Technology of production of pig products. A textbook for the secondary school no. 4. The New Book, 336 p. (in Ukrainian)
24. Panasenko O. I. Synthesis, transformation, physicochemical and biological properties of derivatives of 1,2,4-triazole. Dr. farm sci. thesis, Kyiv, 2005, 396 p. (in Ukrainian)
25. Parchenko V. V., Panasenko O. I., Knysh Y. G. Antiviral activity of salts of 2-[5-(furan-2-yl)-4-R-1,2,4-triazol-3-ylthio] acetate acids. Pharmaceutical Journal, 2008, vol. 6, pp. 79–85.
26. Savchenkova L. V. Clinical pharmacology of thiotriazolinum. Ukrainian Medical Almanac, 2008, vol. 11, issue 3, pp. 212–217. (in Ukrainian)
27. Taimatsukov A. A. Lozeval at poultry diseases. An inform sheet, Krasnodar, 2003, p. 126. (in Russian)
28. Taimazukov A. A., Onischuk F. D., Miroshnichenko V. P. Application of the preparation of loselyum in poultry farming. An inform sheet, Krasnodar, 1999, no. 136, p. 99. (in Russian)
29. Todoriuk V. B., Gutyj B. V., Khomyk R. I., Vasiv R. O. Influence of Ferrovet 7.5 % and Ferosel T on the concentration of mineral substances in the blood serum of piglets suffering from iron deficit anemia. Scientific Messenger of LNUVMBT named after S. Gzhytsky, 2016, vol. 18, issue 3 (71), pp. 139–143. DOI: 10.15421/nvlvet7131. (in Ukrainian)
30. Trofimchuk A.M., Features of the functioning of other factors of resistance of piglets to the action of a thymus drug. The author’s abstract. diss. for the sciences. degree of candidate agr. sciences (03.00.13) “Physiology”, Lviv, 2003, 20 p. (in Ukrainian)
31. Ushkova Y. F. Natural resistance of sows and obtained from them piglets at action of “Interflock” preparation. Scientific messenger of the LNUVMBT named after S. Gzhytsky, 2011, vol. 13, issue 2 (48), part 1, pp. 284–288. Available at: https://nvlvet.com.ua/index.php/journal/issue/view/48/48 (in Ukrainian)
32. Ushkova Y. F., Vischur O. I. Natural resistance of early-born pigs to the action of the drug “Interflock”. Scientific and technical bulletin of State Scientific Research Control Institute of Veterinary Medical Products and Fodder Additives and Institute of Animal Biology, 2009, vol. 10, issue 1–2, pp. 259–262. (in Ukrainian)

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