THE ADDITION OF STIMULATION SCHEME WITH ANTI-ESTERASE SUBSTANCES IMPROVES THE EWE LAMBING RATE
I. V. Lobachova, V. S. Yakovchuk
This email address is being protected from spambots. You need JavaScript enabled to view it.
Institute of Animal Husbandry in Steppe Regions “Ascania-Nova” named after M. F. Ivanov’s — National Scientific Selection-Genetic Centre of Sheep Breeding,
1 Soborna str., Ascania-Nova, Chaplynskyy district, Kherson region, 75230, Ukraine
Most breeds of sheep exhibit seasonality of reproduction. To make it possible to inseminate outside the estrous season, ewes are subjected to hormonal stimulation. The basic scheme of stimulation includes the treatment with gonadotropins after preliminary gestagenization. The most suitable form of prolonged saturation of the body with gestagens is the vaginal sponge. However, the effectiveness of such a scheme is not always sufficient. It is suggested that one of the reasons may be irritation of the vaginal walls with the material of sponge, and one of the ways to reduce this is the treatment of animals with substances that affect the different parts of the nervous system. The purpose of the experiment was to compare the effectiveness of the basic scheme of sexual activity stimulation in sheep during anoestrous season with the scheme that was added by the use of a substance with anticholinesterase activity “Proserine”. For this purpose 2 groups of 2-year-old ewes (control and experimental) were formed and kept together. Gestagenization of all animals was initiated on 11 April by the insertion of sponges with gestagen (Cronolone, 30 mg/ sp., “Intervet”), which were removed on 23 April. To stimulate folliculogenesis, on April 22 all animals were injected with 500 IU of the “Sergon” (PMSG, Czech Republic) and 1 ml of 2.5 % oil solution of progesterone. In addition, all animals were twice treated with 2.5 ml of “SuperAmino-C” (South Korea), which is a mixture of essential amino acids and B vitamins, in the days of insertion and withdrawal of sponges. “Proserine” (0.05 % solution) was injected the experimental animals at a dose 1 ml per ewe per injection twice — in the days of insertion and withdrawal of sponges. All animals were artificially inseminated with freshly obtained semen one time per day during three consecutive days, starting at the next day after sponge withdrawal, without the previous heat testing. In the experimental group, 91.6 % of animals lambed, in the control one — 70.6 %. The prolificacy in the experimental group was 1.18±0.19 vs. 1.68±0.34 lambs/ewe in the control. The fecundity at lambing in the experimental group was 108.5 % vs. 118.5 % in the control, at weaning at 2-months old — 83.3 % vs. 74.1 %. The results of the experiment showed that addition to the basic scheme the 2-fold treatment with the antiesterase substance “Proserine” reduces prolificacy, but increases the efficiency of insemination and improves the survival of lambs to weaning that generally contributes to the increased efficiency of hormonal stimulation in sheep. It was made assumption that the positive effect of the neurotropic substance was able to manifest itself due to the simultaneous saturation of the animal organism with a mixture of essential amino acids and B vitamins.
Keywords: EWE, HEAT, HORMONAL STIMULATION, PROSERINE
- Anderson M., Connors J. M., Hardy S. L., Valent M., Goodman R. L. Thyroid hormones mediate steroid-independent seasonal changes in luteinizing hormone pulsatility in the ewe. Biology of Reproduction, 2002, vol. 66, issue 3, pp. 701–706. https://doi.org/10.1095/biolreprod66.3.701
- Atkinson S., Adams N. R., Martin G. B. Role of progesterone in ovarian follicular growth and development in the sheep. Animal Production in Australia, 1998, vol. 22, pp. 233–236. Available at: http://www.livestocklibrary.com.au/bitstream/handle/1234/8855/Atkinson98.PDF?sequence=1
- Boscos C. M., Samartzi F. C., Dellis S., Rogge A., Stefanakis A., Krambovitis E. Use of progestagen-gonadotrophin treatments in estrus synchronization of sheep. Theriogenology, 2002, vol. 58, issue 7, pp. 1261–1272. https://doi.org/10.1016/S0093-691X(02)01040-3
- Breen K. M., Oakley A. E., Pytiak A. V., Tilbrook A. J., Wagenmaker E. R., Karsch F. J. Does cortisol acting via the type II glucocorticoid receptor mediate suppression of pulsatile luteinizing hormone secretion in response to psychosocial stress? Endocrinology, 2007, vol. 148, issue 4, pp. 1882–1890. https://doi.org/10.1210/en.2006-0973
- Breen K. M., Stackpole C. A., Clarke I. J., Pytiak A. V., Tilbrook A. J., Wagenmaker E. R., Young E. A., Karsch F. J. Does the type II glucocorticoid receptor mediate cortisol-induced suppression in pituitary responsiveness to gonadotropin-releasing hormone? Endocrinology, 2004, vol. 145, issue 6, pp. 2739–2746. https://doi.org/10.1210/en.2004-0123
- Christensen A. C. M., Haresign W., Khalid M. Progesterone exposure of seasonally anoestrous ewes alters the expression of angiogenic growth factors in preovulatory follicles. Theriogenology, 2014, vol. 81, issue 2, pp. 358–367. https://doi.org/10.1016/j.theriogenology.2013.10.005
- Dutt R. H. Induction of estrus and ovulation in anestrual ewes by use of progesterone and pregnant mare serum. Journal of Animal Science, 1953, vol. 12, issue 3. pp. 515–523. https://doi.org/10.2527/jas1953.123515x
- DeNicolo G. Accelerated and out-season lamb production in New Zealand. A thesis for the PhD degree, Massey University, Palmerston North, New Zealand, 2007.
- Graham J. D., Clarke C. L. Physiological action of progesterone in target tissues. Endocrine Reviews, 1997, vol. 18, issue 4, pp. 502–519. https://doi.org/10.1210/edrv.18.4.0308
- Karsch F. J., Dahl G. E., Hachigian T. M., Thrun L. A. Involvement of thyroid hormones in seasonal reproduction. Journal of Reproduction and Fertility. Supplement,1995, vol. 49, pp. 409–422.
- Keefe G. P., Wichtel J. J. Evaluation of melengestrol acetate and equine chorionic gonadotropin for out-of-season breeding in sheep on Prince Edward Island. The Canadian Veterinary Journal, 2000, vol. 41, issue 3, pp. 211–214.
- Manes J., Hozbor F., Alberio R., Ungerfeld R. Intravaginal placebo sponges affect negatively the conception rate in sheep. Small Ruminant Research, 2014, vol. 120, issue 1, pp. 108–111. https://doi.org/10.1016/j.smallrumres.2014.05.006
- Manes J., Fiorentino M. A., Martino S. S., Ungerfeld R. Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle. Livestock Science, 2018, vol. 208, pp. 55–59. https://doi.org/10.1016/j.livsci.2017.11.023
- Mayerhofer A., Fritz S. Ovarian acetylcholine and muscarinic receptors: hints of a novel intrinsic ovarian regulatory system. Microscopy Research and Technique, 2002, vol. 59, issue 6, pp. 503–508. https://doi.org/10.1002/jemt.10228
- Metodiev N., Raicheva E. Short term progestagen treatment for estrus synchronization at nulliparous ewes from the synthetic population Bulgarian milk. Journal of International Scientific Publications: Agriculture and Food, 2014, vol. 2, pp. 382–386. Available at: https://www.scientific-publications.net/get/1000000/1401624906577877.pdf
- Rutte J., Lucero S., Key S., Daniels M., Rodriquez F., Yim H.S. Ovine estrus synchronization and superovulation using norgestomet B and follicle stimulating hormone-pituitary. Theriogenology, 1988, vol. 30, issue 2, pp. 421–427. https://doi.org/10.1016/0093-691X(88)90190-2
- Sirjani M. A., Shakir M. H., Kohram H., Eskanderinasab M. P., Moakhar H. K. The effects of pregnant mare serum gonadotropin (PMSG) injection a day prior or at controlled intravaginal drug-releasing (CIDR) removal on multiple births in Afshari ewes. African Journal of Biotechnology, 2011, vol. 10, issue 57, pp. 12363–12367. Available at: https://www.ajol.info/index.php/ajb/article/download/96416/85743
- Steffl M., Schweiger M., Wessler I., Kunz L., Mayerhofer A., Amselgruber W. M. Non-neuronal acetylcholine and choline acetyltransferase in oviductal epithelial cells of cyclic and pregnant pigs. Anatomy and Embryology, 2006, vol. 211, pp. 685–690. https://doi.org/10.1007/s00429-006-0132-y
- Topurko Z. S. Biotechnological methods of oestrus induction and multiple births in sheep with use of biologically active substances. Cand. agricultural sci. diss., Lviv, 2008. (in Ukrainian)
- Urra J., Blohberger J., Tiszavari M., Mayerhofer A., Lara H. E. In vivo blockade of acetylcholinesterase increases intraovarian acetylcholine and enhances follicular development and fertility in the rat. Scientific Reports, 2016, vol. 6, article number 30129. https://doi.org/10.1038/srep30129
- S. Lamb Resource Center. Accelerated Lambing Cycles, 2016. Available at: http://lambresourcecenter.com/wp-content/uploads/2016/11/ALB-Productivity-Fact-Sheet-ACCELERATED-LAMBING-CLREV-10-14-16.pdf
- Wagenmaker E. R., Breen K. M., Oakley A. E., Tilbrook A. J., Karsch F. J. Psychosocial stress inhibits amplitude of gonadotropin-releasing hormone pulses independent of cortisol action on the type II glucocorticoid receptor. Endocrinology, 2009, vol. 150, issue 2, pp. 762–769. https://doi.org/10.1210/en.2008-0757
- Webster J. R., Moenter S. M., Woodfill C. J., Karsch F. J. Role of the thyroid gland in seasonal reproduction. II. Thyroxine allows a season-specific suppression of gonadotropine secretion in sheep. Endocrinology, 1991, vol. 129, issue 1, pp. 176–183. https://doi.org/10.1210/endo-129-1-176
- Wessler I. K., Kirkpatrick C. J. Non-neuronal acetylcholine involved in reproduction in mammals and honeybees. Journal of Neurochemistry, 2017, vol. 142, issue S2, pp. 144–150. https://doi.org/10.1111/jnc.13953
- Wheaton J. E., Carlson K. M., Windels H. F., Johnson L. J. CIDR: A new progesterone-releasing intravaginal device for induction of estrus and cycle control in sheep and goats. Animal Reproduction Science, 1993, vol. 33, issue 1–4, pp. 127–141. https://doi.org/10.1016/0378-4320(93)90111-4
- WO/2000/033840. Use of acetylcholinesterase inhibitors on the modulation of the hypothalamic-pituitary-gonadal axis. A61K 31/195, Davis Bonnie, Dat. Publ. 15.06.2000.