Bìol. Tvarin, 2019, volume 21, issue 4, pp. 18–21

SOME INDEXES OF CARBOHYDRATE METABOLISM IN MICE UNDER ACTION OF MODIFIED PECTIN

A. Vovkohon

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Bila Tserkva National Agrarian University,
8/1 Soborna sq., Bila Tserkva, Kyiv region, 09117, Ukraine

The search for new and improvement of approved carriers for biotechnological methods and processes of immobilized enzymes and microorganisms’ cells is quite actual. The mostly applied are those carriers which are widely available and not expensive. Also, the carriers used in the food industry should be non-toxic and metabolized or quickly excreted from the organism. The physical and chemical properties of pectin as a food additive allow using it as natural carrier. However, to increase the sorption properties of pectin, at the Research Institute of food technologies and technologies of animal products processing of Bila Tserkva national agrarian university a technology of its modification has been developed. The harmlessness indexes of pectin were studied on white mice. After the experiment on the harmlessness ended, some carbohydrate metabolism indexes were defined for the mice body under the action of different doses of modified pectin. Three groups of experimental animals with body weight 19–20 g were created containing 5 animals in each group. The studies on the content of glucose, pyruvic and lactic acids were conducted in the blood serum. The glycogen content was studied in the liver of linear mice. The feeding, maintenance and keeping of mice during experiment were carried out according to the modern bioethics requirements. It has been found out that at the end of experiment (the day 11 after the intragastric infusion of 0.3 cm3 5.0 % modified pectin suspension), there were no changes as to the glucose, pyruvic and lactic acids in the blood serum and glycogen in liver compared to the animals infused by the physiological solution. The was no probable increase of glucose, pyruvic and lactic acids in the blood serum and glycogen in the liver of mice infused by 0.3 cm3 10.0 % of modified pectin suspension compared to the animals of control group. Thus, there was identified no violation of carbohydrate metabolism in the mice bodies after one-time infusion of 0.3 cm3 5.0 % and 10.0 % modified pectin suspension, indicating that there is no toxic effect of the test substance on the body of white mice.

Keywords: MODIFIED PECTIN, MICE, GLUCOSE, PYRUVIC ACID, LACTIC ACID, GLYCOGEN

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