Bìol. Tvarin. 2021; 23 (2): 12–18.
Received 05.04.2021 ▪ Accepted 16.05.2021 ▪ Published online 01.07.2021

Prooxidant-antioxidant homeostasis and reproductive capacity of boars under the influence of copper citrate

A. S. Siabro

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Poltava State Agrarian Academy,
1/3 Skovorody str., Poltava, 36003, Ukraine

Peroxide oxidation processes play a leading role in ensuring the motility, survival and fertilizing ability of sperm. A special role is given to limiting antioxidants (vitamins, amino acids, microelements). Therefore, the development of standardized feeding programs to provide antioxidant nutrition is one of the effective methods of reproductive biotechnology. The aim of the study was to determine the effect of copper citrate on the quality of sperm production and the formation of prooxidant-antioxidant homeostasis in sperm of boars. The experiment used adult boars of a large white breed, analogs in age, live weight and quality of sperm products. Experimental groups were fed copper citrate above the norm by 10% and 20%. It has been determined that feeding combined feed to boars with the addition of this compound in an amount of 10% above norm probably increases the weight of ejaculate by 12.5% (P<0.05), the sperm motility and survival by 6.5% (P<0.01) and 13.5% (P<0.001), respectively. Such changes in sperm occur against the background of an increase in SOD activity by 80.6% (P<0.05), a decrease in catalase by 43.5% (P<0.05), a slowing down of peroxidation processes — a decrease in diene conjugates and TBA-active compounds. The additional introduction to the diet of copper citrate by 20% more than normal increases the concentration of spermatozoa by 13.2% (P<0.01), the number of live spermatozoa by 20.7% (P<0,01), with a simultaneous decrease in their survival, due to the acceleration of peroxidation processes — an increase in the content of diene conjugates, TBA-active compounds and DAA and a decrease in reduced glutathione. It has been found out that the fertilizing ability of sperm significantly depended on the amount of fed microelement. Sows inseminated with sperm of boars receiving copper supplement in the diet by 10%, had higher fertility rates by 7.1%, multifertility by 3.6%, and a litter weight at weaning by 8.8%. The additional administration of copper citrate reduced the fertility of sperm by 20%, as the fertility rate of sows of III group was the lowest and was 7.7% and 14.3% lower compared to I and II groups. A similar trend occurred in terms of high fertility, a litter weight at birth and weaning. Therefore, the additional feeding of a small amount of copper has a positive effect on the functional activity of sperm and the processes of normal fertilization, growth and development of embryos and newborn piglets by optimizing the formation of prooxidant-antioxidant homeostasis.

Key words: boars, copper citrate, sperm production, peroxide oxidation, reproduction

  1. Espinosa CD, Stein HH. Digestibility and metabolism of copper in diets for pigs and influence of dietary copper on growth performance, intestinal health, and overall immune status: a review. J. Anim. Sci. Biotechnol. 2021; 12: 13. DOI: 10.1186/s40104-020-00533-3.
  2. Kaydashev IP. Handbook of Experimental and Clinical Research in Biology and Medicine. Poltava, 1996: 123–128. (in Ukrainian)
  3. Kolesnikova LI, Kurashova NA, Grebenkina LA, Dolgih MI, Vlasov BY, Neronova NA, Kirilenko EA. Superoxide dismutase and glutathione-dependent enzymes in sperm of men with chronic monotrichonadal infection. Bulletin VSNTS SB RAMS, 2010; 6 (76): 34–36. (in Russian)
  4. Korolyuk MA, Ivanova LI, Majorova IG, Tokarev EV. Method for determining the activity of catalase. Lab. Work. 1988: 1: 16–19. (in Russian)
  5. Kosov NA. The use of chelated compounds of trace elements in pigs feeding. Zootech. Sci. Belarus. 2020: 368–373. (in Belarussian)
  6. Kovalenko VF, Shostya AM, Usenko SO. Method for accelerated determination of C content and its isomers in boar semen. Patent UA no. 67054A. 15.06.2004. (in Ukrainian)
  7. Martins VED, Pinto SCC, Chaves RM, Barros Filho AKD, Laskoski LM, Souza, FA. Antioxidant effect on viability of boar semen cooled to 15°C. Arq. Bras. Med. Vet. Zootec. 2020; 72 (1): 145–152. DOI: 10.1590/1678-4162-11294.
  8. Melnik YF. Instructions for Artificial Insemination of Pigs. Kyiv, Agrarian Science. 2003. (in Ukrainian)
  9. Ogórek M, Gąsior Ł, Pierzchała O, Daszkiewicz R, Lenartowicz M. Role of copper in the process of spermatogenesis. Postepy Hig. Med. Dosw. 2017; 71: 662–680. DOI: 10.5604/01.3001.0010.3846.
  10. Parrilla I, Martinez, EA, Gil MA, Cuello C, Roca J, Rodriguez-Martinez H, Martinez CA. Boar seminal plasma: current insights on its potential role for assisted reproductive technologies in swine. Anim. Reprod. 2020; 17 (3). DOI: 10.1590/1984-3143-ar2020-0022.
  11. Podufalij VV, Cherkashina IV, Kuchkov IN. Processes of lipid peroxidation in the active-mobile fraction of human sperm isolated before and after cryopreservation. Probl. Cryobiol. 2008; 18 (4): 520–523. (in Russian)
  12. Ribalko VP. Modern Research Methods in Pig Breeding. Poltava, 2005: 114–123. (in Ukrainian)
  13. Roblero L, Guadarrama A, Lopez T, Zegers-Hochschild F. Effect of copper ion on the motility, viability, acrosome reaction and fertilizing capacity of human spermatozoa in vitro. Reproduction, Fertility and Development. 1996; 8 (5): 871–874. DOI: 10.1071/RD9960871.
  14. Rokotyanska VO. Features of prooxidant-antioxidant homeostasis in the semen of breeding boars with correction of vitamin and mineral nutrition. Diss. Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies, Lviv; 2020: 159 p. (in Ukrainian)
  15. Roychoudhury S, Nath S, Massanyi P, Stawarz R, Kacaniova M, Kolesarova A. Copper-induced changes in reproductive functions: in vivo and in vitro effects. Physiol. Res. 2016; 65: 11–22. DOI: 10.33549/physiolres.933063.
  16. Shabunin SV. Methodological provisions for the study of free radical oxidation processes in the antioxidant defense system of the body. Voronezh, 2010: 36–37; 51–52. (in Russian)
  17. Surai PF, Fisinin VI. Selenium in pig nutrition and reproduction: boars and semen quality — a review. AJAS. 2015; 28 (5): 730–746. DOI: 10.5713/ajas.14.0593.
  18. Tvrda E, Peer R, Sikka SC, Agarwal A. Iron and copper in male reproduction: a double-edged sword. J. Assist. Reprod. Genet. 2015; 32 (1): 3–16. DOI: 10.1007/s10815-014-0344-7.
  19. Usenko SO, Shostya AM, Stoyanovskij VG, Birta GO, Kuzmenko LM, Slynko VG. Prooxidant-antioxidant homeostasis in the incubated semen of breeding boars during feeding of lactates of microelements. Sci. Rep. NULES of Ukraine. 2020; 2 (84): 14 p. DOI: 10.31548/dopovidi2020.02.017. (in Ukrainian)
  20. Vongpralub T, Thananurak P, Ssttikasamkit C, Chuawongboon P, Duangjinda M, Boonkum W, Chankitisakul V. Comparison of effects of different antioxidants supplemented to long-term extender on boar semen quality following storage at 17°C. Thai J. Vet. Med. 2016; 46 (1): 119–126. Available at: https://he01.tci-thaijo.org/index.php/tjvm/article/view/49791