Bìol. Tvarin. 2021; 23 (4): 43–48.
Received 07.10.2021 ▪ Accepted 03.12.2021 ▪ Published online 29.12.2021

Biochemical markers of hepatobiliary system functional state in rats under the action of laser irradiation and ω-3 polyunsaturated fatty acids

O. V. Ketsa, I. Y. Kapitanchuk

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Yuriy Fedkovych Chernivtsi National University,
2 Kotsyubynskoho str., Chernivtsi, 58012, Ukraine

Markers of the functional state of the hepatobiliary system — enzymatic activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), γ-glutamyltransferase (GGT), levels of total and direct bilirubin, the value of thymol probe in the plasma of the diaphragm waves and the introduction of ω-3 PUFA were investigated. It has been found that the action of laser irradiation in blood plasma increases the enzymatic activities of aminotransferases and GGT, along with an increase in the level of total and direct bilirubin and thymol index. The corrective effect on the liver functional state is shown by ω-3 polyunsaturated fatty acids (PUFA). It has been established that the protective properties of ω-3 PUFAs depend on the scheme of their introduction. The highest hepatoprotective effect of ω-3 PUFAs is manifested under the conditions of their previous introduction to the action of laser irradiation, as evidenced by a decrease in hyperenzymemia ALT, AST, GGT, a decrease in total and direct bilirubin and thymol in plasma. The introduction of ω-3 PUFA after the end of the laser diode does not change the markers of the functional state of the liver in blood plasma compared with irradiated animals that were not injected with the studied lipophilic nutrients.

Key words: rats, liver, alanine aminotransferase, aspartate aminotransferase, γ-glutamyltransferase, bilirubin, laser irradiation, ω-3 polyunsaturated fatty acids

  1. Ayee MAA, Bunker BC, De Groot JL. Membrane modulatory effects of omega-3 fatty acids: Analysis of molecular level interactions. Top. Membr. 2020; 86: 57–81. DOI: 10.1016/bs.ctm.2020.08.001.
  2. Bissig KD, Han W, Barzi M, Kovalchuk N, Ding L, Fan X, Pankowicz FP, Zhang QY, Ding X. P450-humanized and human liver chimeric mouse models for studying xenobiotic metabolism and toxicity. Drug Metab. Dispos. 2018; 46 (11): 1734–1744. DOI: 10.1124/dmd.118.083303.
  3. Chen L, Chen R, Kemper S, Cong M, You H, Brigstock DR. Therapeutic effects of serum extracellular vesicles in liver fibrosis. Extracell. Vesicles. 2018; 7 (1): 1461505. DOI: 10.1080/20013078.2018.1461505.
  4. De Avelar CR, Pereira EM, de Farias Costa PR, de Jesus RP, de Oliveira LPM. Effect of silymarin on biochemical indicators in patients with liver disease: Systematic review with meta-analysis. World J. Gastroenterol. 2017; 23 (27): 5004–5017. DOI: 10.3748/wjg.v23.i27.5004.
  5. Jampa-Ngern S, Viravaidya-Pasuwat K, Suvanasuthi S, Khantachawana A. Effect of laser diode light irradiation on growth capability of human hair follicle dermal papilla cells. 39th Int. Conf. IEEE Eng. Med. Biol. Soc. 2017: 3592–3595. DOI: 10.1109/EMBC.2017.8037634.
  6. Kunkel HG, Hoagland Mechanism and significance of the thymol turbidity test for liver disease. J. Clin. Invest. 1947; 26 (6): 1060–1071. DOI: 10.1172/JCI101898.
  7. Kwo PY, Cohen SM, Lim JK. ACG clinical guideline: evaluation of abnormal liver chemistries. J. Gastroenterol. 2017; 112 (1): 18–35. DOI: 10.1038/ajg.2016.517.
  8. Lozano-Paniagua D, Parrón T, Alarcón R, Requena M, López-Guarnido O, Lacasaña M, Hernández AF. Evaluation of conventional and non-conventional biomarkers of liver toxicity in greenhouse workers occupationally exposed to pesticides. Food Chem. Toxicol. 2021; 151: 112127. DOI: 10.1016/j.fct.2021.112127.
  9. Michalopoulos GK, Bhushan B. Liver regeneration: biological and pathological mechanisms and implications. Rev. Gastroenterol. Hepatol. 2021; 18 (1): 40–55. DOI: 10.1038/s41575-020-0342-4.
  10. Moss DW, Henderson AR. Clinical enzymology. In: Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical Chemistry. 3rd Philadelphia, W.B Saunders Company, 1999: 617–721.
  11. Muster B, Rapp A, Cardoso MC. Systematic analysis of DNA damage induction and DNA repair pathway activation by continuous wave visible light laser micro-irradiation. AIMS Genet. 2017; 4 (1): 47–68. DOI: 10.3934/genet.2017.1.47.
  12. Schumann G, Bonora R, Ceriotti F, Férard G, Ferrero CA, Franck PFH, Gella FJ, Hoelzel W, Jørgensen PJ, Kanno T, Kessner A, Klauke R, Kristiansen N, Lessinger JM, Linsinger TPJ, Misaki H, Panteghini M, Pauwels J, Schiele F, Schimmel HG. IFCC Primary reference procedures for the measurements of catalytic activity concentrations of enzymes at 37°C. Part 6. Reference procedure for the measurements of catalytic concentration of γ-glutamyltransferase. Chem. Lab Med. 2002; 40 (7): 734–738. DOI: 10.1515/CCLM.2002.126.
  13. Shahidi F, Ambigaipalan P. Omega-3 polyunsaturated fatty acids and their health benefits. Rev. Food Sci. Technol. 2018; 9: 345–381. DOI: 10.1146/annurev-food-111317-095850.
  14. Shobha R, Narayanan VS, Jagadish Pai BS, Jaishankar HP, Jijin MJ. Low-level laser therapy: A novel therapeutic approach to temporomandibular disorder — A randomized, double-blinded, placebo-controlled trial. Indian J. Dent. Res. 2017; 28 (4): 380–387. DOI: 10.4103/ijdr.IJDR_345_15.
  15. Shurygina IP, Zilov VG, Smekalkina LV, Naprienko MB, Safonov MI, Akulov SN. Effect of infrared low-intensity laser irradiation on lipid peroxidation under conditions of experimental circulatory hypoxia of visual analyzer. Exp. Biol. Med. 2020; 168 (5): 602–604. DOI: 10.1007/s10517-020-04760-6.
  16. Simmons NA. An automated method for serum bilirubin determination. Clin. Path. 1968; 21: 196–201. DOI: 10.1136/jcp.21.2.196.
  17. Sullivan EM, Pennington ER, Green WD, Beck MA, Brown DA, Shaikh SR. Mechanisms by which dietary fatty acids regulate mitochondrial structure-function in health and disease. Nutr. 2018; 9 (3): 247–262. DOI: 10.1093/advances/nmy007.
  18. Thomas L. Alanine aminotransferase (ALT), aspartate aminotransferase (AST). In: Clinical Laboratory Diagnostics. 1st Frankfurt: TH‑Books Verlagsgesellschaft, 1998: 55–65.






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