Efficiency of “Biolide” disinfectant working solutions for action on gram-negative and gram-positive bacteria

O. N. Chechet1, V. L. Kovalenko2, T. O. Garkavenko1, O. I. Gorbatyuk1, T. H. Kozytska1

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1State Research Institute of Laboratory Diagnostics and Veterinary and Sanitary Examination,
30 Donetska str., Kyiv, 03151, Ukraine

2State Scientific Control Institute of Biotechnology and Strains of Microorganisms,
30 Donetska str., Kyiv, 03151, Ukraine

The article presents the results of the new developed disinfectant “Biolide” research for use in poultry and other sectors of agriculture, because today the problem of developing cheap and effective disinfectants remains relevant. In recent years, the poultry industry has a tendency to rapid development, since the population’s demands for poultry products have increased markedly. With an integrated approach to the production of chicken meat, it is possible to increase the productivity of poultry enterprises, energy efficiency and reduce the cost of production. When applying such an integrated approach, one of the important roles is played by the provision of high-quality disinfection with effective and inexpensive means. In connection with the relevance of the development of new effective disinfectants, the main purpose of the research was to determine the effectiveness of working solutions in concentrations of 0.1; 0.2; 0.25 and 0.5% for gram-negative E. coli ATCC 25922 and gram-positive S. aureus ATCC 25923 for different periods of time — 20, 30, 60 and 120 min. after the simulation of protein contamination. Test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 in lyophilized form were stored in a refrigerator at a temperature –70±5°C. By replacing them on nutrient media, metabolic processes were restored and their correspondence to the main typical properties for this type of pathogens was checked. Simulation of protein contamination was carried out using sterile inactivated blood serum of cattle in the amount of 40.0% to the volume of the used bacterial suspension. In laboratory tests, smooth surfaces of tiles with an area 100 cm2 were used as test objects. The analysis of the obtained research results showed the high efficiency of 0.5% working solutions of the new disinfectant “Biolide” when exposed to test cultures E. coli ATCC 25922 and S. aureus ATCC 25923 for 60 min., since this concentration of the working disinfectant solution and the exposure time ensured the destruction by 99.99–100.0% of gram-negative and gram-positive microorganisms when imitating protein contamination of test objects.

Key words: “Biolide” disinfectant, working solutions, protein contamination, E. coli ATCC 25922 test culture, S. aureus ATCC 25923 test culture, test objects

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