the functional activity Changes in RAT’s neutrophils in experimental collagen ARTHRITIS
I. Y. Kril1, A. M. Havrylyuk1, V. V. Chopyak1, Yu. Ya. Kit2, A. V. Kotsiuruba3,R. S. Stoika2
1Danylo Halytsky Lviv National Medical University, Pekarska St., 69b,
Lviv, 79010, Ukraine,This email address is being protected from spambots. You need JavaScript enabled to view it.
2Institute of Cell Biology, NAS ofUkraine, Drahomanov St., 14/16,
Lviv, 79005, Ukraine, This email address is being protected from spambots. You need JavaScript enabled to view it.
3Bogomoletz Institute of Physiology, NAS ofUkraine, Bogomoletz St., 4,
01024, Kiev, Ukraine,This email address is being protected from spambots. You need JavaScript enabled to view it.
There were investigated the functional changes in rat neutrophils in experimental collagen arthritis, which is a model of rheumatoid arthritis (RA) in humans. There were examined the absorption capacity of neutrophils in experimental animals in tests of spontaneous and stimulated phagocytosis, oxidative activity explosion (spontaneous and stimulated) by flow cytometry and concentration of free oxygen radicals in the blood serum of these animals. Native blood of experimental animals were incubated with FITC-labeled E.coli bacteria in the presence of various stimuli or not, and the relative number of cells that have absorbed the bacteria. It was found that the increased spontaneous phagocytosis absorptivity of neutrophils, indicating a pronounced activation of these cells and its reduction after stimulation with E. coli compared to the animals control group that may be indicative of neutrophils`s low back ability. During the study of spontaneous and stimulated activity of oxidative explosion in neutrophils it was observed the increase of the parameters in the cases of low physiological stimulation and without it. In the same time it was observed some stimulation of E.coli and strong PMA stimulation that led to their decrease. The neutrophils digestion process weakening indicated an increased the bacterial infections risk, which subsequently may lead to autoimmunization. Free oxygen radicals were formed during oxidative explosion that were toxical on the cell membrane. The experimental group of animals showed a high concentration of free oxygen radicals compared with intact animals group. These data confirm the high pathologic changes likelihood in the experimental model of RA.
Keywords: COLLAGEN ARTHRITIS, WHITE LABORATORY RATS, NEUTROPHILS, PHAGOCYTIC ACTIVITY, OXIDATIVE EXPLOSION, FREE OXYGEN RADICALS
