Bìol. Tvarin, 2019, volume 21, issue 4, pp. 84–90


O. O. Chabanenko, N. A. Yershova, N. V. Orlova, N. M. Shpakova

This email address is being protected from spambots. You need JavaScript enabled to view it.

Institute for Problems of Cryobiology and Cryomedicine NAS of Ukraine,
23 Pereyaslavska str., Kharkiv 61016, Ukraine

The effect of sodium decyl sulfate and chlorpromazine on the sensitivity of rat and rabbit red blood cells to post-hypertonic shock has been studied in this research. Post-hypertonic shock has a stressful impact on cells when they are transferred from hypertonic (dehydration medium) into a physiological solution (rehydration medium). The use of post-hypertonic shock of red blood cells allows the investigation of the influence of cryopreservation factors, acting at the stage of cell thawing as well as when transferring into the bloodstream of red blood cells cryopreserved under the protection of penetrating cryoprotective agent. It has been shown that at 0 °C the level of post-hypertonic lysis of rat and rabbit red blood cells does not differ and is 60 %, at the same time at 37 °C, rabbit red blood cells are more resistant to post-hypertonic shock than rat’s cells.It has been established that the effect of amphiphiles on red blood cells of animals in post-hypertonic shock depends on temperature. At 37 °C the protective effect of the studied amphiphilic compounds was not detected. The performance of post-hypertonic shock of erythrocytes at 0 °C using both anionic sodium decyl sulfate and cationic chlorpromazine significantly reduces the level of post-hypertensive lysis of red blood cells of both species. At the same time, negatively charged sodium decyl sulfate exhibits higher anti-hemolytic activity for rabbit erythrocytes (70 %) versus the rat ones (56 %). Positively charged chlorpromazine is more effective for rat erythrocytes. The revealed protective effect of amphiphilic compounds during post-hypertonic shock of red blood cells at 0 °C is assumed to be related to the state of red blood cell membrane at low temperature. Under these conditions, the membrane components are less mobile and molecules of amphiphilic compounds, building-into the bilayer, can “fix” the microdefects formed during the dehydration stage and prevent against their increasing up to the hemolytic pore size at the rehydration stage.


  1. Acker J. P., Hansen A. L., Kurach J. D., Turner T. R., Croteau I., Jenkins C. A. A quality monitoring program for red blood cell components: in vitro quality indicators before and after implementation of semiautomated processing. Transfusion, 2014, vol. 54, issue 10, pp. 2534–2543. https://doi.org/10.1111/trf.12679
  2. Benga G. Comparative studies of water permeability of red blood cells from humans and over 30 animal species: an overview of 20 years of collaboration with Philip Kuchel. European Biophysics Journal, 2013, vol. 42, pp. 33–46. https://doi.org/10.1007/s00249-012-0868-7
  3. Chabanenko O. O., Orlova N. V., Shpakova N. M. Glycerol and posthypertonic shock of erythrocytes when varing medium temperature and osmolality. Cryobiology, 2018, vol. 85, p. 179. https://doi.org/10.1016/j.cryobiol.2018.10.223
  4. Chen J. Y., Brunauer L. S., Chu F. C., Helsel C. M., Gedde M. M., Huestis W. H. Selective amphipathic nature of chlorpromazine binding to plasma membrane bilayers. Biochimica et Biophysica Acta (BBA) — Biomembranes, 2003, vol. 1616, issue 1, pp. 95–105. https://doi.org/10.1016/S0005-2736(03)00229-3
  5. Ershova N. A., Shpakova N. M., Orlova N. V. Effect of phenylhydrazine and alkylsulfates on osmotic sensitivity of mammalian erythrocytes. Reports of the National Academy of Sciences of Ukraine, 2012, no. 6, pp. 129–133. (in Russian)
  6. Ficarra S., Russo A., Barreca D., Giunta E., Galtieri A., Tellone E. Short-term effects of chlorpromazine on oxidative stress in erythrocyte functionality: activation of metabolism and membrane perturbation. Oxidative Medicine and Cellular Longevity, 2016, ID 2394130, 10 p. https://doi.org/10.1155/2016/2394130
  7. Fuller B. J., Benson E. E. (eds). Life in the frozen state. Boca Raton, London, New York, Washington, D.C., CRC Press, 2004, 672 p.
  8. Henkelman S., Lagerberg J. W., Graaff R., Rakhorst G., Van Oeveren W. The effect of cryopreservation on red blood cell rheologic properties. Transfusion, 2010, vol. 50, issue 11, pp. 2393–2401. https://doi.org/10.1111/j.1537-2995.2010.02730.x
  9. Isomaa B., Hägerstrand H., Paatero G. Shape transformations induced by amphiphiles in erythrocytes. Biochimica et Biophysica Acta (BBA) — Biomembranes, 1987, vol. 899, issue 1, pp. 93–103. https://doi.org/10.1016/0005-2736(87)90243-4
  10. Jiang Y.-W., Gao G., Chen Z., Wu F.-G. Fluorescence studies on the interaction between chlorpromazine and model cell membranes. New Journal of Chemistry, 2017, vol. 41, issue 10, pp. 4048–4057. https://doi.org/10.1039/C7NJ00037E
  11. Manaargadoo-Catin M., Ali-Cherif A., Pougnas J.-L., Perrin C. Hemolysis by surfactant — a review. Advances in Colloid and Interface Science, 2015, vol. 228, pp. 1–16. https://doi.org/10.1016/j.cis.2015.10.011
  12. Muldrew K., Schachar J., Cheng P., Rempel C., Liang S., Wan R. The possible influence of osmotic poration on cell membrane water permeability. Cryobiology, 2009, vol. 58, issue 1, pp. 62–68. https://doi.org/10.1016/j.cryobiol.2008.10.129
  13. Nicolson G. L. The fluid — mosaic model of membrane structure: still relevant to understanding the structure, function and dynamics of biological membranes after more than 40 years. Biochimica et Biophysica Acta (BBA) — Biomembranes, 2014, vol. 1838, issue 6, pp. 1451–1466. https://doi.org/10.1016/j.bbamem.2013.10.019
  14. Oleynik O. A., Ramazanov V. V., Bondarenko V. A. Posthypertonic lysis of modified erythrocytes in citrate medium. Problems of Cryobiology, 2003, issue 3, pp. 21–29.
  15. Semionova E. A., Chabanenko E. O., Orlova N. V., Zubov P. M., Shpakova N. M. About mechanism of antihemolytic action of chlorpromazine under posthypertonic stress in erythrocytes. Problems of Cryobiology and Cryomedicine, 2017, vol. 27, issue 3, pp. 219–229. https://doi.org/10.15407/cryo27.03.219
  16. Shpakova N. M., Ershov S. S., Nipot O. E. To the question about possible correlation between release of K+ ions and development of hemolytic damage of mammalian erythrocytes under hypertonic cryohemolysis. The Animal Biology, 2008, vol. 10, issue 1–2, pp. 164–170. (in Ukrainian) Available at: http://archive.inenbiol.com.ua:8080/bt/2008/2/10.pdf
  17. Shpakova N. M., Iershova N. A., Orlova N. V., Iershov S. S., Synchykova O. P. Application of alkyl sulfates and heat treated erythrocytes in hypertonic cryohemolysis. Biotechnologia Acta, 2015, vol. 8, issue 3, pp. 129–136. https://doi.org/10.15407/biotech8.03.129
  18. Shpakova N.M., Orlova N.V., Yershov S.S. Correction of cold damage to mammalian erythrocytes by chlorpromazine to influence the dynamic structure of a membrane. Biophysics, 2019, vol. 64, no. 3, pp. 367–373. (in Russian) https://doi.org/10.1134/S0006350919030205
  19. Sobotka H., Stewart C. P. Advanced in clinical chemistry. Vol. 8. London, New York, Academic Press, 1965, 335 p.
  20. Tacheva B., Paarvanova B., Ivanov I. T., Tenchov B, Georgieva R., Karabaliev M. Drug exchange between albumin nanoparticles and erythrocyte membranes. Nanomaterials, 2019, vol. 9, issue 1, article 47, 14 p. https://doi.org/10.3390/nano9010047
  21. Virtanen J. A., Cheng K. H., Somerharju P. Phospholipid composition of the mammalian red cell membrane can be rationalized by a superlattice model. Proceedings of the National Academy of Sciences of the United States of America, 1998, vol. 95, issue 9, pp. 4964–4969. https://doi.org/10.1073/pnas.95.9.4964

Download full text in PDF






WorldCat Logo