Bìol. Tvarin, 2013, volume 15, issue 3, pp. 22–30
https://doi.org/10.15407/animbiol15.03.022
PHYSIOLOGICAL EFFECTS OF SOYBEANS NATIVE AND TRANSGENIC VARIETIES ON THE BODY OF THE THIRD GENERATION FEMALE RATSInstitute of Animal Biology NAAS,
Laboratory of ecological physiology and quality of productions,
38 V. Stus str., Lviv 79034, Ukraine
As a result of considerable progress in biotechnology in recent years more crops and raw materials obtained from genetically modified organisms, including soybeans, are part of the feed and food. In spite of long use of GMOs in these areas, their impact on animal organisms is controversial and understudied, especially in dynamic generations, that caused choice the direction of our research.In this article provided the results of studies of the physiological state of the third-generation female rats, under conditions of feeding them and their mothers conventional and genetically modified soybeans in an amount of 30 % of the nutritional value of the ration. We investigated that effect of soy manifested an increase in blood levels of the investigated glycoprotein and immunological parameters. This indicating the activation of immune system and could be caused by nutritional composition of soybean and the presence in it of biologically active compounds with the immunostimulatory effects.The results of determination of phenols fractions indicate us about tension of detoxification processes in the animal’s organisms of research groups.The installed higher transaminase activity of heart and liver tissue with a decrease its activity in the blood that may indicate a positive effect of soybeans on liver cells, including membrane integrity and the highest intensity of protein metabolism in the animal’s organisms of research groups. A generalized analysis of the results of research conducted on female rats leads to the conclusion that there is no definite negative or positive impact of GM soy components on their physiological state compared with animals fed native soybeans.
Keywords: GLYCOPROTEINS; CIRCULATING IMMUNE COMPLEXES; MOLECULES OF AVERAGE WEIGHT; AMINOTRANSFERASE ACTIVITY; SOYBEANS
1. Tutelyan V. A., Pavlyuchkova M. S., Pogozheva A. V., Derbeneva S. A. The use of phytoestrogens in medicine. Nutritional issues, 2003, no. 2, pp. 48–54. (in Russian)
2. Kaprelyants L. V., Kiselev S. V., Iorgacheva Ye. G. Soy isoflavones and the prospects for their therapeutic use. Nutritional issues, 2003, no 4, pp. 36–41. (in Russian)
3. Gordiyenko A. D. Hepatoprotective mechanism of action of flavonoids. An overview. Pharmacy, 1990, no. 3, pp. 75–78. (in Russian)
4. Saraf A. S., Oganesyan E. T. Flavonoids as potential anti-allergic compounds. An overview. Pharmaceutical chemistry journal, 1991, no. 2, pp. 4–8. (in Russian)
5. Prokopenko L. G., Chalyy G. A. Proteolytic enzymes and their inhibitors as modulators of immune responses. Pharmacology and Toxicology, 1987, no. 5, pp. 93–99. (in Russian)
6. Synovets A. S., Levitskiy A. P. Inhibitors of proteolytic enzymes in medicine. Kyiv, Zdorovya, 1985, 71 p. (in Russian)
7. Padgette S. R., Taylor N. B., Nida D. L., Bailey M. R., MacDonald J., Holden L. R., Fuchs R. L. The composition of glyphosate-tolerant soybean seeds is equivalent to that of conventional soybeans. J. Nutr., 1996, 126 (3), pp. 702–716. https://doi.org/10.1093/jn/126.3.702
8. Patrushev M. V., Voznyak M. V. Genetically modified sources: characteristics of some GM-lines, their detection. Partners and Competitors, 2004, no. 10, pp. 19–26. (in Russian)
9. Paranyak R. P., Vudmaska V. I., Paranyak M. R., Kulchytsky V. V. Amino and fatty acids substantional equivalence of genetically modified (gts 40-3-2) and traditional soybeans. Achievements of Clinical and Experimental Medicine, 2009, vol. 10, no. 1, pp. 117–120. (in Ukrainian)
10. European convention for the protection of vertebrate animals used forexperim. and other scientific purposes.Coun. of Europe, Strasbourg, 1986, p. 53.
11. Vlizlo V. V. Laboratory research methods in biology, animal husbandry and veterinary medicine. Lviv, Spolom, 2012, pp. 355–368. (in Ukrainian)
12. Grinevich Yu. A. Determination of immune complexes in the blood of cancer patients. Lab. Business, 1981, no. 8, pp. 493–496. (in Russian)
13. Gottshalk A. Glycoproteins. Moscow, World, 1969, 303 p. (in Russian)
14. Khyuz R. Glycoproteins. Moscow, World, 1985, 140 p. (in Russian)
15. Pilatte Y. Bignon J., Lambre C. R. Sialicacids as important molecules in the regulation of the immune system: pathophysiological implications of sialidascs in immunity. Glycobiology, 1993, 3 (3), pp. 201–217. https://doi.org/10.1093/glycob/3.3.201
16. Montreuil J. Spatial conformation of glycans and glycoproteins. Biology of the Cell, 1984, 51, pp. 115–131. https://doi.org/10.1111/j.1768-322X.1984.tb00291.x
17. Sharon N. Glycoproteins. Trends in Biochemical Sciences, 1984, 9, pp. 198–202. https://doi.org/10.1016/0968-0004(84)90139-7
18. Cook G. M. W. Cell surface carbohydrates: molecules in search for a function Cell Sci., 1986, 82 (Suppl. 4), pp. 45–70.
19. Teshima R., Akiyama H., Okunuki H., Sakushima J.-I., Goda Y., Onodera H., Sawada J.-I., Toyoda M. Effect of GM and non-GM soybeans on the immune system of BN rats and B10a mice. Journal of Food Hygiene Society Japan, 2000, 41, p. 188–193. https://doi.org/10.3358/shokueishi.41.188
20. Appenzeller L. M., Munley S. M., Hoban D., Sykes G. P., Malley L. A., Delaney B. Subchronic feeding study of herbicide-tolerant soybean DP-356Ø43-5 in Sprague–Dawley rats. Food Chem. Toxicol., 2008, 46 (16), pp. 2201–2213. https://doi.org/10.1016/j.fct.2008.02.017
21. Qi X., He X., Luo Y., Li S., Zou S., Cao S., Tang M., Delaney B., Xu W., Huang K. Subchronic feeding study of stacked trait genetically-modified soybean (3Ø5423 × 40-3-2) in Sprague–Dawley rats. Food and Chemical Toxicology, 2012, 50 (9), pp. 3256–3263. https://doi.org/10.1016/j.fct.2012.06.052
22. Zhu Y., Li D., Wang F., Yin J., Jin H. Nutritional assessment and fate of DNA of soybean meal from Roundup Ready or conventional soybeans using rats. Arch. Anim. Nutr., 2004, 58, pp. 295–310. https://doi.org/10.1080/00039420412331273277
23. Malyk O. H., Kotsyumbas I. Ya. Phytoestrogens. Lviv, Dobra sprava, 2006, 140 p. (in Ukrainian)
24. Anosike C. A., Obidoa O., Ezeanyika L. U. S. Beneficial Effects of Soybean Diet on Serum Marker Enzymes, Lipid Profile and Relative Organ Weights of Wistar Rats. Pakistan Journal of Nutrition, 2008, 7 (6), pp. 817–822. https://doi.org/10.3923/pjn.2008.817.822
25. Tudisco R., Lombardi P., Bovera F., d’Angelo D., Cutrignelli M. I., Mastellone V., Terzi V., Avallone L., Infascelli F. Genetically modified soya bean in rabbit feeding: detection of DNA fragments and evaluation of metabolic effects by enzymatic analysis. Animal Science, 2006, 82, pp. 193–199. https://doi.org/10.1079/ASC200530
26. Tudisco R., Mastellone V., Cutrignelli M. I., Lombardi P., Bovera F., Mirabella N., Piccolo G., Calabro S., Avallone L., Infascelli F. Fate of transgenic DNA and evaluation of metabolic effects in goats fed genetically modified soybean and in their offsprings. Animal, 2010, 4 (10), pp. 1–10. https://doi.org/10.1017/S1751731110000728
27. Cîrnatu D., Jompan A., Sin A. I., Zugrav C. A. Multiple organ histopathological changes in broiler chickens fed on genetically modified organism. Rom. J. Morphol. Embryol., 2011, 52 (Suppl. 1), pp. 475–480.
