Hypertonic cryohemolysis in bovine, equine, canine and human erythrocytes: osmotic and temperature peculiarities

N. M. Shpakova, S. S. Ershov, N. V. Orlova

Hypertonic cryohemolysis in human and animal (bovine, equine and canine) erythrocytes has been compared. Cryohemolysis decrease with time in human, equine and canine erythrocytes is observed in electrolyte medium (1.2 mol/l NaCl) in contrast to a non-electrolyte (0.86 mol/l sucrose) one. This reduction is associated with a probable flux of Na+ extracellular ions into cells and as the consequence with osmotic gradient diminishing on a membrane at the moment of temperature shift from 37 to 0°C. Decrease with time in hypertonic cryohemolysis for bovine erythrocytes was revealed when salt concentration in medium increased up to 2.1 mol/l NaCl. There were demonstrated the features of mammalian species in erythrocyte responses when varying temperature of incubation medium (37, 25, 15°C) and with following cell cooling down to 0°C. High resistance of bovine erythrocytes to hypertonic cryohemolysis (in comparison with the studied cells of other mammals) can be associated with high content in membrane of cholesterol and sphingomyelin, capable of suppress cug a defect formation in erythrocyte membranes.

Keywords: mammalian erythrocytes, hypertonic cryohemolysis, medium temperature, medium osmolarity

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