STUDY AND PАTHOGENIC STRAINS IMUEOHENNYH PROPERTIES
E. COLI IBM-1 IN THE DEVELOP NEW IMMUNOGENIC DRUGS
T. B. Vasileva, A. A. Zaviriuha
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SSI «State center of innovation biotechnologies»,
Donetska str., 30, Kyiv-03151
In the prevention of colibacillosis of calves achieved some success, but the problem is still far from its full resolution. The results of the study of pathogenic and immunogenic properties of a strain of E. coli IBM-1 daly basis for the development of new drugs. The aim was to compare the immunogenic properties of the toxins of different strains of E. coli, to examine the ability of E. coli strain exotoxin IBM-1 cause an immune response and to investigate the state of natural resistance on indicators opsono-phagocytic reaction serum after immunization vaccine «Metakol». Infection was performed daily cultures of the respective strains to determine the lethal dose. Found that in comparison with other field strains of E. coli strain E. coli IBM-1 causes 100 % killing of laboratory animals after parenteral dosing LD100 = 850 x 106 CFU/c 3. Results from the study of immunogenic properties of the vaccine «Metakol» show that animals immunized with vaccine research groups comprising the E. coli strain IBM-1 with protection against colibacillosis by 70–80 %. Established that immunization of heifers before calving vaccine «Metakol» activates the process of phagocytosis by 7 % and its intensity by 6 % compared with unvaccinated livestock heifers. It was established that the drug has the ability to create specific immunity against other strains of E. coli, as well as a means for the prevention and treatment of colibacillosis of young animals. In the long term continuation of studies on the immunogenic properties of the vaccine strain of E. coli IBM-1 to create new products.
Keywords: PATHOGENICITI, IMMUNOGENICITY, STRAINS, EXOTOXIN, PHAGOCYTOSIS, COLIBACILLOSIS, DRUGS
