MOLECULAR AND GENETIC CHARACTERIStics ISOLATES Virus of porcine enzootic Encephalomyelitis ISOLATED FROM WILD BOAR AND DOMESTIC Swine ON THE TERRITORY OF UKRAINE
M. P. Sytiuk1, V. G. Spyrydonov2, V. O. Postoienko1, S. D. Melnychuk2, V. V. Nedosekov2
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1Institute of Veterinary Medicine of the National Academy of Agrarian Sciences of Ukraine, Donetska st., 30, Kiev, 03151, Ukraine;
2National University of Life and Environmental Sciences of Ukraine,
Heroiv Oborony st., 15, Kiev, 03041, Ukraine
The article provide data about detection two isolates viruses of porcine enzootic encephalomyelitisfrom the territory of Ukraine isolated from domestic piglet and wild boar. Porcine enzootic encephalomyelitisvirus isolate «Shevchenkovo-2005» was collected from domestic piglet in 2005 in private housing on the territory of Kyiv region. The virus isolate «Novoazovsky-2013» was detected from shot wild boar on the territory of Lugansk region. The initial was done the indication of the viruses in cell culture of pig kidney (PK) was performed with their further identification for sensitivity to chloroform. PCR technique was used for detecting of species-specific RNA virus of porcine enzootic encephalomyelitiswith subsequent VP1 cDNA sequencing. The results of estimations showed that above-mentioned samples belong to PTV 1 Teschovirus group. The designed by us primers that would be specific for almost all serotypes of porcine Teschoviruses it was analyzed by nucleotide sequences encoding VP1 of 11 PTV available serotypes. This allowed us for detection of the conservative region and designing of the oligonucleotide primers (PTV-F, PTV-R). Phylogenic analysis of amino acid sequences of capsid protein VP1 of Teschoviruses from Ukrainian isolates and different serotypes have shown that the viruses from Ukraine belong to the first Teschovirus group (PTV1). There were found some point mutations in the isolates may be due to high mutational rate as they are single-strained RNA viruses and its observation is peculiar for all members of Picornaviridae family.
Keywords: TESCHEN DISEASE VIRUSES, DOMESTIC PIGLET, WILD BOAR, IDENTIFICATION, POLYMERASE CHAIN REACTION, SEQUENCING, PHYLOGENIC ANALYSIS
