THE DETERMINATION OF DL50 OF BIOLOGICALLY ACTIVE PRODUCT CONTAINING PEPTIDOGLYCAN
V. I. Kushnir
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State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives, Donets’ka str., 11, Lviv, 79019, Ukraine
The typical peculiarity of medicinal products of microbial origin is their capability to demonstrate antibacterial and immune stimulating properties. In the practice of veterinary medicine they are used aiming at the treatment and prophylaxis of the intestinal tract diseases and enhancing the immune condition of the organism. The tests were conducted on the basis of the pharmacology and toxicology laboratory of State Scientific Research Control Institute of Veterinary Medicinal Products and Feed Additives. The aim of the work was to determine the parameters of the acute toxicity of biologically active product containing peptidoglycan of lactate bacteria and bifidobacteria administered intragastrically and subcutaneously and also to determine their irritating effect on mucous membrane of eye and skin. The tests were conducted using white rats, mice and rabbits.
As it was impossible to determine DL50 of the tested biologically active product administered intragastrically and subcutaneously, the medicinal product was administered in the maximal doses. The conducted tests showed that single intragastrical administration of the medicinal product in the doses of 5000 and 10000 mg/kg and subcutaneous administration in the doses of 1500, 3000 and 5000 mg/kg the death and disease occurrence of laboratory mice and rats were not observed, the signs of poisoning and changes in animal behaviour were not also observed. There were not also the changes in weight coefficients of internal organs. According to the classification of chemical substances on the basis of danger degree the tested medicinal product administered intragastrically belongs to the 4th class (less toxic substances) and due to this classification the tested medicinal product belongs to the 6th class (harmless substances). The medicinal product did not cause the irritating effect as a result of its application to mucous membrane of rabbit eye, index of primary irritation is equal to zero.
Keywords: ACUTE TOXICITY, DL50, DANGER DEGREE, IRRITATING EFFECT, PEPTIDOGLYCAN, LABORATORY MICE AND RATS
