ASSESSMENT OF THE MUTAGENIC ACTIVITE OF OOCYSTS OF CHICKENS EIMERIA IN AMES TEST
V.V.Stybel1, A.Y.Hirkovyy1, M.M.Danko2
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1Lviv National University of Veterinary Medicine and Biotechnologies named after S. Z. Gzhytskyj,
50Pekarska str., Lviv 79010, Ukraine
2State Scientific-Research Control Institute of Veterinary Medicinal Products and Feed Additives,
11Donetska str., Lviv 79019, Ukraine
The mutagenic effects of infective oocysts of chickens eimeria (Eimeria maxima, E. tenella, E. necatrix, E. acervulina) was studied using the Ames test. The method consists in recording the ability of the test substance or its metabolites induce reverse mutations from auxotrophy to prototrophy for histidine in the test strain Salmonella thyphimurium, who are his-mutation and are not able to synthesize histidine.
The studies were conducted at three concentrations — native, diluted in 10 and 100 times. In determining the mutagenic activity of infective oocysts of E. maxima, E. tenella and E. necatrix found that native concentration induced reversion to both strains S. thyphimurium: ТА-98 2,4 and ТА-100 2,3 times higher than in the control. The native concentration of homogenate infective E. acervulina oocysts induce reversion to both strains ТА-98 2,5 and ТА-100 2,3 times higher than in controls. By dilution of the homogenate of infective oocysts of E. maxima, E. tenella, E. necatrix and E. acervulina in 10 and 100 times the induction of gene mutations were detected.
These data suggest that the infective homogenate of of infective oocysts of chickens eimeria contains biologically active substances that possess mutagenic activity weak force (on a scale mutagenicity 1 point) and potentially able to induce reverse mutations to histidine independence in strains S. typhimurium TA-98 and TA-100 for type of shift reading frames and type of replacement pairs.
Keywords:CHICKENS, EIMERIA, OOCYSTS, AMES TEST, MUTAGENICITY
